5vrh - Revision history

OCA at 13:56, 4 October 2023

OCA — Wed, 04 Oct 2023 13:56:27 GMT

←Older revision		Revision as of 13:56, 4 October 2023
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	<StructureSection load='5vrh' size='340' side='right'caption='[[5vrh]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='5vrh' size='340' side='right'caption='[[5vrh]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vrh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5VRH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vrh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VRH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.137Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vrg\|5vrg]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vrh OCA], [~~http~~://pdbe.org/5vrh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vrh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vrh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vrh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vrh OCA], [https://pdbe.org/5vrh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vrh RCSB], [https://www.ebi.ac.uk/pdbsum/5vrh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vrh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI]] Transfers the fatty acyl group on membrane lipoproteins.	+	[https://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI] Transfers the fatty acyl group on membrane lipoproteins.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Murray~~, J M~~]]	+	[[Category: Murray JM]]
-	[[Category: Noland~~, C L]]~~	+	[[Category: Noland CL]]
-	~~[[Category: Acyl transferase]]~~	+
-	~~[[Category: Lnt c387]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:43, 30 September 2020

OCA — Wed, 30 Sep 2020 08:43:43 GMT

←Older revision		Revision as of 08:43, 30 September 2020
Line 1:		Line 1:

	==Apolipoprotein N-acyltransferase C387S active site mutant==		==Apolipoprotein N-acyltransferase C387S active site mutant==
-	<StructureSection load='5vrh' size='340' side='right' caption='[[5vrh]], [[Resolution\|resolution]] 2.14Å' scene=''>	+	<StructureSection load='5vrh' size='340' side='right'caption='[[5vrh]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vrh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VRH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vrh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5VRH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vrg\|5vrg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vrg\|5vrg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vrh OCA], [http://pdbe.org/5vrh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vrh RCSB], [http://www.ebi.ac.uk/pdbsum/5vrh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vrh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vrh OCA], [http://pdbe.org/5vrh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vrh RCSB], [http://www.ebi.ac.uk/pdbsum/5vrh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vrh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Murray, J M]]		[[Category: Murray, J M]]
	[[Category: Noland, C L]]		[[Category: Noland, C L]]

OCA at 09:05, 9 August 2017

OCA — Wed, 09 Aug 2017 09:05:24 GMT

←Older revision		Revision as of 09:05, 9 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vrh is ~~ON HOLD~~	+	==Apolipoprotein N-acyltransferase C387S active site mutant==
		+	<StructureSection load='5vrh' size='340' side='right' caption='[[5vrh]], [[Resolution\|resolution]] 2.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vrh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VRH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vrg\|5vrg]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vrh OCA], [http://pdbe.org/5vrh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vrh RCSB], [http://www.ebi.ac.uk/pdbsum/5vrh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vrh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI]] Transfers the fatty acyl group on membrane lipoproteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-negative bacteria express a diverse array of lipoproteins that are essential for various aspects of cell growth and virulence, including nutrient uptake, signal transduction, adhesion, conjugation, sporulation, and outer membrane protein folding. Lipoprotein maturation requires the sequential activity of three enzymes that are embedded in the cytoplasmic membrane. First, phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (Lgt) recognizes a conserved lipobox motif within the prolipoprotein signal sequence and catalyzes the addition of diacylglycerol to an invariant cysteine. The signal sequence is then cleaved by signal peptidase II (LspA) to give an N-terminal S-diacylglyceryl cysteine. Finally, apolipoprotein N-acyltransferase (Lnt) catalyzes the transfer of the sn-1-acyl chain of phosphatidylethanolamine to this N-terminal cysteine, generating a mature, triacylated lipoprotein. Although structural studies of Lgt and LspA have yielded significant mechanistic insights into this essential biosynthetic pathway, the structure of Lnt has remained elusive. Here, we present crystal structures of wild-type and an active-site mutant of Escherichia coli Lnt. The structures reveal a monomeric eight-transmembrane helix fold that supports a periplasmic carbon-nitrogen hydrolase domain containing a Cys-Glu-Lys catalytic triad. Two lipids are bound at the active site in the structures, and we propose a putative phosphate recognition site where a chloride ion is coordinated near the active site. Based on these structures and complementary cell-based, biochemical, and molecular dynamics approaches, we propose a mechanism for substrate engagement and catalysis by E. coli Lnt.

-	~~Authors: Murray~~, J.M., ~~Noland~~, C.L.	+	Structural insights into lipoprotein N-acylation by Escherichia coli apolipoprotein N-acyltransferase.,Noland CL, Kattke MD, Diao J, Gloor SL, Pantua H, Reichelt M, Katakam AK, Yan D, Kang J, Zilberleyb I, Xu M, Kapadia SB, Murray JM Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6044-E6053. doi:, 10.1073/pnas.1707813114. Epub 2017 Jul 11. PMID:28698362<ref>PMID:28698362</ref>

-	~~Description: Apolipoprotein N~~-~~acyltransferase C387S active site mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Noland, C.L]]	+	<div class="pdbe-citations 5vrh" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Murray, J.M~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Murray, J M]]
		+	[[Category: Noland, C L]]
		+	[[Category: Acyl transferase]]
		+	[[Category: Lnt c387]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transferase]]

OCA: Protected "5vrh" [edit=sysop:move=sysop]

OCA — Wed, 17 May 2017 14:09:51 GMT

Protected "5vrh" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:09, 17 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vrh is ON HOLD Authors: Murray, J.M., Noland, C.L. Description: Apolipoprotein N-acyltransferase C387S active site mutant [[Category: Unreleased S...

OCA — Wed, 17 May 2017 14:09:50 GMT

New page: '''Unreleased structure''' The entry 5vrh is ON HOLD Authors: Murray, J.M., Noland, C.L. Description: Apolipoprotein N-acyltransferase C387S active site mutant [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 5vrh is ON HOLD

Authors: Murray, J.M., Noland, C.L.

Description: Apolipoprotein N-acyltransferase C387S active site mutant
[[Category: Unreleased Structures]]
[[Category: Noland, C.L]]
[[Category: Murray, J.M]]