5vws - Revision history

OCA at 14:01, 4 October 2023

OCA — Wed, 04 Oct 2023 14:01:18 GMT

←Older revision		Revision as of 14:01, 4 October 2023
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	<StructureSection load='5vws' size='340' side='right'caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='5vws' size='340' side='right'caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thebd Thebd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobispora_bispora_DSM_43833 Thermobispora bispora DSM 43833]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VWS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.411Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Tbis_0546 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=469371 THEBD])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vws OCA], [~~http~~://pdbe.org/5vws PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vws RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vws PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vws ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vws OCA], [https://pdbe.org/5vws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vws RCSB], [https://www.ebi.ac.uk/pdbsum/5vws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vws ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D6Y4Z8_THEBD D6Y4Z8_THEBD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thebd~~]]	+	[[Category: Thermobispora bispora DSM 43833]]
-	[[Category: Bowers~~, A A~~]]	+	[[Category: Bowers AA]]
-	[[Category: Brustad~~, E M~~]]	+	[[Category: Brustad EM]]
-	[[Category: Ghodge~~, S V~~]]	+	[[Category: Ghodge SV]]
-	[[Category: Gober~~, J G]]~~	+	[[Category: Gober JG]]
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Thiomuracin gz~~]]	+

OCA at 08:06, 27 November 2019

OCA — Wed, 27 Nov 2019 08:06:06 GMT

←Older revision		Revision as of 08:06, 27 November 2019
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	==Ligand free structure of Cytochrome P450 TbtJ1==		==Ligand free structure of Cytochrome P450 TbtJ1==
-	<StructureSection load='5vws' size='340' side='right' caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>	+	<StructureSection load='5vws' size='340' side='right'caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thebd Thebd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VWS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thebd Thebd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VWS FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5vws" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vws" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thebd]]		[[Category: Thebd]]
	[[Category: Bowers, A A]]		[[Category: Bowers, A A]]

OCA at 23:30, 15 November 2017

OCA — Wed, 15 Nov 2017 23:30:51 GMT

←Older revision		Revision as of 23:30, 15 November 2017
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	<StructureSection load='5vws' size='340' side='right' caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='5vws' size='340' side='right' caption='[[5vws]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thebd Thebd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VWS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tbis_0546 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469371 THEBD])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vws OCA], [http://pdbe.org/5vws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vws RCSB], [http://www.ebi.ac.uk/pdbsum/5vws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vws OCA], [http://pdbe.org/5vws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vws RCSB], [http://www.ebi.ac.uk/pdbsum/5vws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vws ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Thebd]]
	[[Category: Bowers, A A]]		[[Category: Bowers, A A]]
	[[Category: Brustad, E M]]		[[Category: Brustad, E M]]

OCA: Protected "5vws" [edit=sysop:move=sysop]

OCA — Fri, 04 Aug 2017 04:11:53 GMT

Protected "5vws" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 4 August 2017

OCA: New page: ==Ligand free structure of Cytochrome P450 TbtJ1== == Structural ...

OCA — Fri, 04 Aug 2017 04:11:51 GMT

New page: ==Ligand free structure of Cytochrome P450 TbtJ1== <StructureSection load='5vws' size='340' side='right' caption='5vws, resolution 2.41Å' scene=''> == Structural ...

New page

==Ligand free structure of Cytochrome P450 TbtJ1==
<StructureSection load='5vws' size='340' side='right' caption='[[5vws]], [[Resolution|resolution]] 2.41Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vws]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VWS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vws OCA], [http://pdbe.org/5vws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vws RCSB], [http://www.ebi.ac.uk/pdbsum/5vws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vws ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Thiopeptides are a growing class of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Many biosynthetic enzymes for RiPPs, especially thiopeptides, are promiscuous and can accept a wide range of peptide substrates with different amino acid sequences; thus, these enzymes have been used as tools to generate new natural product derivatives. Here, we explore an alternative route to molecular complexity by engineering thiopeptide tailoring enzymes to do new or non-native chemistry. We explore cytochrome P450 enzymes as biocatalysts for cyclopropanation of dehydroalanines, chemical motifs found widely in thiopeptides and other RiPP-based natural products. We find that P450TbtJ1 and P450TbtJ2 selectively cyclopropanate dehydroalanines in a number of complex thiopeptide-based substrates and convert them into 1-amino-2-cyclopropane carboxylic acids (ACCAs), which are important pharmacophores. This chemistry takes advantage of the innate affinity of these biosynthetic enzymes for their substrates and enables incorporation of new pharmacophores into thiopeptide architectures. This work also presents a strategy for diversification of natural products through rationally repurposing biosynthetic enzymes as non-natural biocatalysts.

P450-Mediated Non-natural Cyclopropanation of Dehydroalanine-Containing Thiopeptides.,Gober JG, Ghodge SV, Bogart JW, Wever WJ, Watkins RR, Brustad EM, Bowers AA ACS Chem Biol. 2017 Jul 21;12(7):1726-1731. doi: 10.1021/acschembio.7b00358. Epub, 2017 Jun 1. PMID:28535034<ref>PMID:28535034</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5vws" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bowers, A A]]
[[Category: Brustad, E M]]
[[Category: Ghodge, S V]]
[[Category: Gober, J G]]
[[Category: Cytochrome p450]]
[[Category: Oxidoreductase]]
[[Category: Thiomuracin gz]]