5vyr - Revision history

OCA at 09:43, 1 January 2020

OCA — Wed, 01 Jan 2020 09:43:08 GMT

OCA: Protected "5vyr" [edit=sysop:move=sysop]

OCA — Thu, 21 Sep 2017 04:47:02 GMT

Protected "5vyr" [edit=sysop:move=sysop]

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Revision as of 04:47, 21 September 2017

OCA: New page: ==Crystal structure of the WbkC formyl transferase from Brucella melitensis== == Structural highlights ==
<table><tr><td colspan='2'>[[5vyr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VYR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B62:(6R)-2-AMINO-6-METHYL-5,6,7,8-TETRAHYDROPTERIDIN-4(3H)-ONE'>B62</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vyq|5vyq]], [[5vys|5vys]], [[5vyt|5vyt]], [[5vyu|5vyu]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vyr OCA], [http://pdbe.org/5vyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vyr RCSB], [http://www.ebi.ac.uk/pdbsum/5vyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vyr ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
It has become increasingly apparent within the last several years that unusual N-formylated sugars are often found on the O-antigens of such Gram negative pathogenic organisms as Francisella tularensis, Campylobacter jejuni, and Providencia alcalifaciens, among others. Indeed, in some species of Brucella, for example, the O-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-alpha-d-mannosyl groups. These sugars, often referred to as N-formylperosamine, are synthesized in pathways initiating with GDP-mannose. One of the enzymes required for the production of N-formylperosamine, namely, WbkC, was first identified in 2000 and was suggested to function as an N-formyltransferase. Its biochemical activity was never experimentally verified, however. Here we describe a combined structural and functional investigation of WbkC from Brucella melitensis. Four high resolution X-ray structures of WbkC were determined in various complexes to address its active site architecture. Unexpectedly, the quaternary structure of WbkC was shown to be different from that previously observed for other sugar N-formyltransferases. Additionally, the structures revealed a second binding site for a GDP molecule distinct from that required for GDP-perosamine positioning. In keeping with this additional binding site, kinetic data with the wild type enzyme revealed complex patterns. Removal of GDP binding by mutating Phe 142 to an alanine residue resulted in an enzyme variant displaying normal Michaelis-Menten kinetics. These data suggest that this nucleotide binding pocket plays a role in enzyme regulation. Finally, by using an alternative substrate, we demonstrate that WbkC can be utilized to produce a trideoxysugar not found in nature.

Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.,Riegert AS, Chantigian DP, Thoden JB, Tipton PA, Holden HM Biochemistry. 2017 Jul 18;56(28):3657-3668. doi: 10.1021/acs.biochem.7b00494., Epub 2017 Jul 5. PMID:28636341<ref>PMID:28636341</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5vyr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Chantigian, D P]]
[[Category: Holden, H M]]
[[Category: Riegert, A S]]
[[Category: Thoden, J B]]
[[Category: Deoxysugar]]
[[Category: N-formyltransferase]]
[[Category: Transferase]]