5vzr - Revision history

OCA at 14:03, 4 October 2023

OCA — Wed, 04 Oct 2023 14:03:40 GMT

←Older revision		Revision as of 14:03, 4 October 2023
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	<StructureSection load='5vzr' size='340' side='right'caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>		<StructureSection load='5vzr' size='340' side='right'caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VZR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VZR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.57Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vzr OCA], [~~http~~://pdbe.org/5vzr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vzr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vzr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vzr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vzr OCA], [https://pdbe.org/5vzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vzr RCSB], [https://www.ebi.ac.uk/pdbsum/5vzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vzr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Sandbox 20009\|Sandbox 20009]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: McLellan~~, J S~~]]	+	[[Category: McLellan JS]]
-	[[Category: Wang, N]]	+	[[Category: Wang N]]
-	[[Category: Wrapp, D]]	+	[[Category: Wrapp D]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Neutralizing~~]]	+

OCA at 17:26, 11 December 2019

OCA — Wed, 11 Dec 2019 17:26:55 GMT

←Older revision		Revision as of 17:26, 11 December 2019
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	==Crystal Structure of MERS-CoV neutralizing antibody G4 Fab==		==Crystal Structure of MERS-CoV neutralizing antibody G4 Fab==
-	<StructureSection load='5vzr' size='340' side='right' caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>	+	<StructureSection load='5vzr' size='340' side='right'caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VZR FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VZR FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5vzr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vzr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: McLellan, J S]]		[[Category: McLellan, J S]]

OCA at 14:43, 6 November 2017

OCA — Mon, 06 Nov 2017 14:43:44 GMT

←Older revision		Revision as of 14:43, 6 November 2017
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	<StructureSection load='5vzr' size='340' side='right' caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>		<StructureSection load='5vzr' size='340' side='right' caption='[[5vzr]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VZR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VZR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: McLellan, J S]]		[[Category: McLellan, J S]]
	[[Category: Wang, N]]		[[Category: Wang, N]]

OCA: Protected "5vzr" [edit=sysop:move=sysop]

OCA — Wed, 30 Aug 2017 03:56:03 GMT

Protected "5vzr" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:56, 30 August 2017

OCA: New page: ==Crystal Structure of MERS-CoV neutralizing antibody G4 Fab== ==...

OCA — Wed, 30 Aug 2017 03:56:01 GMT

New page: ==Crystal Structure of MERS-CoV neutralizing antibody G4 Fab== <StructureSection load='5vzr' size='340' side='right' caption='5vzr, resolution 1.57Å' scene=''> ==...

New page

==Crystal Structure of MERS-CoV neutralizing antibody G4 Fab==
<StructureSection load='5vzr' size='340' side='right' caption='[[5vzr]], [[Resolution|resolution]] 1.57Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vzr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VZR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vzr OCA], [http://pdbe.org/5vzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vzr RCSB], [http://www.ebi.ac.uk/pdbsum/5vzr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vzr ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Middle East respiratory syndrome coronavirus (MERS-CoV) is a lineage C betacoronavirus that since its emergence in 2012 has caused outbreaks in human populations with case-fatality rates of approximately 36%. As in other coronaviruses, the spike (S) glycoprotein of MERS-CoV mediates receptor recognition and membrane fusion and is the primary target of the humoral immune response during infection. Here we use structure-based design to develop a generalizable strategy for retaining coronavirus S proteins in the antigenically optimal prefusion conformation and demonstrate that our engineered immunogen is able to elicit high neutralizing antibody titers against MERS-CoV. We also determined high-resolution structures of the trimeric MERS-CoV S ectodomain in complex with G4, a stem-directed neutralizing antibody. The structures reveal that G4 recognizes a glycosylated loop that is variable among coronaviruses and they define four conformational states of the trimer wherein each receptor-binding domain is either tightly packed at the membrane-distal apex or rotated into a receptor-accessible conformation. Our studies suggest a potential mechanism for fusion initiation through sequential receptor-binding events and provide a foundation for the structure-based design of coronavirus vaccines.

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen.,Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS Proc Natl Acad Sci U S A. 2017 Aug 14. pii: 201707304. doi:, 10.1073/pnas.1707304114. PMID:28807998<ref>PMID:28807998</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5vzr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: McLellan, J S]]
[[Category: Wang, N]]
[[Category: Wrapp, D]]
[[Category: Antibody]]
[[Category: Immune system]]
[[Category: Neutralizing]]