5w0t - Revision history

OCA at 09:39, 4 December 2019

OCA — Wed, 04 Dec 2019 09:39:46 GMT

←Older revision		Revision as of 09:39, 4 December 2019
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	==Crystal structure of monomeric Msp1 from S. cerevisiae==		==Crystal structure of monomeric Msp1 from S. cerevisiae==
-	<StructureSection load='5w0t' size='340' side='right' caption='[[5w0t]], [[Resolution\|resolution]] 2.63Å' scene=''>	+	<StructureSection load='5w0t' size='340' side='right'caption='[[5w0t]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br>		<table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Keenan, R J]]		[[Category: Keenan, R J]]
	[[Category: Mateja, A M]]		[[Category: Mateja, A M]]

OCA at 10:32, 22 November 2017

OCA — Wed, 22 Nov 2017 10:32:18 GMT

←Older revision		Revision as of 10:32, 22 November 2017
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	<StructureSection load='5w0t' size='340' side='right' caption='[[5w0t]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='5w0t' size='340' side='right' caption='[[5w0t]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSP1, YTA4, YGR028W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0t OCA], [http://pdbe.org/5w0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0t RCSB], [http://www.ebi.ac.uk/pdbsum/5w0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0t OCA], [http://pdbe.org/5w0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0t RCSB], [http://www.ebi.ac.uk/pdbsum/5w0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0t ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Keenan, R J]]		[[Category: Keenan, R J]]
	[[Category: Mateja, A M]]		[[Category: Mateja, A M]]

OCA: Protected "5w0t" [edit=sysop:move=sysop]

OCA — Fri, 04 Aug 2017 04:13:10 GMT

Protected "5w0t" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 4 August 2017

OCA: New page: ==Crystal structure of monomeric Msp1 from S. cerevisiae== == Str...

OCA — Fri, 04 Aug 2017 04:13:07 GMT

New page: ==Crystal structure of monomeric Msp1 from S. cerevisiae== <StructureSection load='5w0t' size='340' side='right' caption='5w0t, resolution 2.63Å' scene=''> == Str...

New page

==Crystal structure of monomeric Msp1 from S. cerevisiae==
<StructureSection load='5w0t' size='340' side='right' caption='[[5w0t]], [[Resolution|resolution]] 2.63Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0t OCA], [http://pdbe.org/5w0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0t RCSB], [http://www.ebi.ac.uk/pdbsum/5w0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0t ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MSP1_YEAST MSP1_YEAST]] Involved in intramitochondrial sorting of proteins.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mislocalized tail-anchored (TA) proteins of the outer mitochondrial membrane are cleared by a newly identified quality control pathway involving the conserved eukaryotic protein Msp1 (ATAD1 in humans). Msp1 is a transmembrane AAA-ATPase, but its role in TA protein clearance is not known. Here, using purified components reconstituted into proteoliposomes, we show that Msp1 is both necessary and sufficient to drive the ATP-dependent extraction of TA proteins from the membrane. A crystal structure of the Msp1 cytosolic region modeled into a ring hexamer suggests that active Msp1 contains a conserved membrane-facing surface adjacent to a central pore. Structure-guided mutagenesis of the pore residues shows that they are critical for TA protein extraction in vitro and for functional complementation of an msp1 deletion in yeast. Together, these data provide a molecular framework for Msp1-dependent extraction of mislocalized TA proteins from the outer mitochondrial membrane.

Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins.,Wohlever ML, Mateja A, McGilvray PT, Day KJ, Keenan RJ Mol Cell. 2017 Jul 20;67(2):194-202.e6. doi: 10.1016/j.molcel.2017.06.019. Epub, 2017 Jul 14. PMID:28712723<ref>PMID:28712723</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5w0t" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Keenan, R J]]
[[Category: Mateja, A M]]
[[Category: Wohlever, M L]]
[[Category: Aaa atpase]]
[[Category: Hydrolase]]