5w0v - Revision history

OCA at 14:04, 4 October 2023

OCA — Wed, 04 Oct 2023 14:04:40 GMT

←Older revision		Revision as of 14:04, 4 October 2023
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	<StructureSection load='5w0v' size='340' side='right'caption='[[5w0v]], [[Resolution\|resolution]] 2.82Å' scene=''>		<StructureSection load='5w0v' size='340' side='right'caption='[[5w0v]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5W0V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W0V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [~~http~~://pdbe.org/5w0v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.821Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [https://pdbe.org/5w0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [https://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6CMF0_KLULA Q6CMF0_KLULA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Kluyveromyces lactis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: An, S]]	+	[[Category: An S]]
-	[[Category: Cho, U S]]	+	[[Category: Cho U-S]]
-	[[Category: Song, J J]]	+	[[Category: Song J-J]]
-	[[Category: Yoon, J]]	+	[[Category: Yoon J]]
-	~~[[Category: 23 heat repeats with a right-handed superhelical solenoid structure]]~~	+
-	~~[[Category: Budding yeast karyopherin]]~~	+
-	~~[[Category: Histone nls recognition]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: The extra-long helix of the repeat 23~~]]	+

OCA at 08:46, 30 September 2020

OCA — Wed, 30 Sep 2020 08:46:46 GMT

←Older revision		Revision as of 08:46, 30 September 2020
Line 1:		Line 1:

	==Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34==		==Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34==
-	<StructureSection load='5w0v' size='340' side='right' caption='[[5w0v]], [[Resolution\|resolution]] 2.82Å' scene=''>	+	<StructureSection load='5w0v' size='340' side='right'caption='[[5w0v]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W0V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5W0V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [http://pdbe.org/5w0v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [http://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [http://pdbe.org/5w0v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [http://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: An, S]]		[[Category: An, S]]
	[[Category: Cho, U S]]		[[Category: Cho, U S]]

OCA at 06:09, 1 November 2017

OCA — Wed, 01 Nov 2017 06:09:03 GMT

←Older revision		Revision as of 06:09, 1 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5w0v is ~~ON HOLD until May 31 2019~~	+	==Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34==
		+	<StructureSection load='5w0v' size='340' side='right' caption='[[5w0v]], [[Resolution\|resolution]] 2.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5w0v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0V FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0v OCA], [http://pdbe.org/5w0v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0v RCSB], [http://www.ebi.ac.uk/pdbsum/5w0v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0v ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation.

-	~~Authors:~~ An, S., Yoon, J., Song, J.-J.~~, Cho, U~~.-S.	+	Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123.,An S, Yoon J, Kim H, Song JJ, Cho US Elife. 2017 Oct 16;6. pii: e30244. doi: 10.7554/eLife.30244. PMID:29035199<ref>PMID:29035199</ref>

-	~~Description: Crystal structure~~ of ~~full~~-~~length Kluyveromyces lactis Kap123 with histone H4 1~~-34	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5w0v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: An, S]]		[[Category: An, S]]
-	[[Category: ~~Song~~, ~~J.-J~~]]	+	[[Category: Cho, U S]]
-	[[Category: ~~Cho~~, ~~U.-S~~]]	+	[[Category: Song, J J]]
	[[Category: Yoon, J]]		[[Category: Yoon, J]]
		+	[[Category: 23 heat repeats with a right-handed superhelical solenoid structure]]
		+	[[Category: Budding yeast karyopherin]]
		+	[[Category: Histone nls recognition]]
		+	[[Category: Protein transport]]
		+	[[Category: The extra-long helix of the repeat 23]]

OCA: Protected "5w0v" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:18:58 GMT

Protected "5w0v" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:18, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5w0v is ON HOLD until May 31 2019 Authors: An, S., Yoon, J., Song, J.-J., Cho, U.-S. Description: Crystal structure of full-length Kluyveromyces la...

OCA — Wed, 27 Sep 2017 14:18:57 GMT

New page: '''Unreleased structure''' The entry 5w0v is ON HOLD until May 31 2019 Authors: An, S., Yoon, J., Song, J.-J., Cho, U.-S. Description: Crystal structure of full-length Kluyveromyces la...

New page

'''Unreleased structure'''

The entry 5w0v is ON HOLD until May 31 2019

Authors: An, S., Yoon, J., Song, J.-J., Cho, U.-S.

Description: Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34
[[Category: Unreleased Structures]]
[[Category: An, S]]
[[Category: Song, J.-J]]
[[Category: Cho, U.-S]]
[[Category: Yoon, J]]