5w2g - Revision history

OCA at 14:05, 4 October 2023

OCA — Wed, 04 Oct 2023 14:05:39 GMT

←Older revision		Revision as of 14:05, 4 October 2023
Line 1:		Line 1:

	==Crystal structure of the core catalytic domain of human inositol phosphate multikinase==		==Crystal structure of the core catalytic domain of human inositol phosphate multikinase==
-	<StructureSection load='5w2g' size='340' side='right' caption='[[5w2g]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5w2g' size='340' side='right'caption='[[5w2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w2g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w2g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W2G FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5w2h\|5w2h]], [[5w2i\|5w2i]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">IPMK, IMPK ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2g OCA], [https://pdbe.org/5w2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w2g RCSB], [https://www.ebi.ac.uk/pdbsum/5w2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2g ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2g OCA], [~~http~~://pdbe.org/5w2g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w2g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w2g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IPMK_HUMAN IPMK_HUMAN]] Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4).<ref>PMID:12027805</ref> <ref>PMID:12223481</ref>	+	[https://www.uniprot.org/uniprot/IPMK_HUMAN IPMK_HUMAN] Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4).<ref>PMID:12027805</ref> <ref>PMID:12223481</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Shears, S B~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wang, H~~]]	+	[[Category: Shears SB]]
-	[[Category: ~~Inositol]]~~	+	[[Category: Wang H]]
-	~~[[Category: Inositol polyphosphate]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Phosphatidylinositol]]~~	+
-	~~[[Category: Specificity]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:16, 15 November 2017

OCA — Wed, 15 Nov 2017 10:16:08 GMT

←Older revision		Revision as of 10:16, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5w2g' size='340' side='right' caption='[[5w2g]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5w2g' size='340' side='right' caption='[[5w2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w2g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w2g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W2G FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w2h\|5w2h]], [[5w2i\|5w2i]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w2h\|5w2h]], [[5w2i\|5w2i]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IPMK, IMPK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2g OCA], [http://pdbe.org/5w2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w2g RCSB], [http://www.ebi.ac.uk/pdbsum/5w2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2g OCA], [http://pdbe.org/5w2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w2g RCSB], [http://www.ebi.ac.uk/pdbsum/5w2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2g ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Shears, S B]]		[[Category: Shears, S B]]
	[[Category: Wang, H]]		[[Category: Wang, H]]

OCA: Protected "5w2g" [edit=sysop:move=sysop]

OCA — Thu, 21 Sep 2017 04:45:19 GMT

Protected "5w2g" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:45, 21 September 2017

OCA: New page: ==Crystal structure of the core catalytic domain of human inositol phosphate multikinase== == Structural highlights ==
<table><tr><td colspan='2'>[[5w2g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W2G FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w2h|5w2h]], [[5w2i|5w2i]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2g OCA], [http://pdbe.org/5w2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w2g RCSB], [http://www.ebi.ac.uk/pdbsum/5w2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2g ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/IPMK_HUMAN IPMK_HUMAN]] Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4).<ref>PMID:12027805</ref> <ref>PMID:12223481</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human inositol phosphate multikinase (HsIPMK) critically contributes to intracellular signaling through its inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) 3-kinase and phosphatidylinositol- 4,5-bisphosphate (PtdIns(4,5)P2) 3-kinase activities. This catalytic profile is not conserved; orthologs from Arabidopsis thaliana and Saccharomyces cerevisiae are predominantly Ins(1,4,5)P3 6-kinases, and the plant enzyme cannot phosphorylate PtdIns(4,5)P2. Therefore, crystallographic analysis of the yeast and plant enzymes, without bound inositol phosphates, do not structurally rationalize HsIPMK activities. Here, we present 1.6 A resolution crystal structures of HsIPMK in complex with either Ins(1,4,5)P3 or PtdIns(4,5)P2. The Ins(1,4,5)P3 headgroup of PtdIns(4,5)P2 binds in precisely the same orientation as free Ins(1,4,5)P3 itself, indicative of evolutionary optimization of 3- kinase activities against both substrates. We report on nucleotide binding between the separate N- and C-lobes of HsIPMK. The N-lobe exhibits a remarkable degree of conservation with protein kinase A (root mean square deviation = 1.8 A), indicating common ancestry. We also describe structural features unique to HsIPMK. First, we observed a constrained, horseshoe-shaped substrate pocket, formed from an alpha-helix, a 310 helix, and a recently evolved tri-proline loop. We further found HsIPMK activities rely on a preponderance of Gln residues, in contrast to the larger Lys and Arg residues in yeast and plant orthologs. These conclusions are supported by analyzing 14 single-site HsIPMK mutants, some of which differentially affect 3-kinase and 6- kinase activities. Overall, we structurally rationalize phosphorylation of Ins(1,4,5)P3 and PtdIns(4,5)P2 by HsIPMK.

Structural features of human inositol phosphate multikinase rationalize its inositol phosphate kinase and phosphoinositide 3-kinase activities.,Wang H, Shears SB J Biol Chem. 2017 Sep 7. pii: jbc.M117.801845. doi: 10.1074/jbc.M117.801845. PMID:28882892<ref>PMID:28882892</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5w2g" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Shears, S B]]
[[Category: Wang, H]]
[[Category: Inositol]]
[[Category: Inositol polyphosphate]]
[[Category: Kinase]]
[[Category: Phosphatidylinositol]]
[[Category: Specificity]]
[[Category: Transferase]]