5w34 - Revision history

OCA at 14:06, 4 October 2023

OCA — Wed, 04 Oct 2023 14:06:03 GMT

←Older revision		Revision as of 14:06, 4 October 2023
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	==Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with double-stranded DNA GACCGGAAGTGG==		==Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with double-stranded DNA GACCGGAAGTGG==
-	<StructureSection load='5w34' size='340' side='right' caption='[[5w34]], [[Resolution\|resolution]] 2.95Å' scene=''>	+	<StructureSection load='5w34' size='340' side='right'caption='[[5w34]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w34]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W34 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W34 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w34]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W34 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5w33\|5w33]], [[5w35\|5w35]], [[5w36\|5w36]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.95Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">dnaG, Rv2343c, MTCY98~~.~~12c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83332 MYCTU])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w34 OCA], [https://pdbe.org/5w34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w34 RCSB], [https://www.ebi.ac.uk/pdbsum/5w34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w34 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w34 OCA], [~~http~~://pdbe.org/5w34 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w34 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w34 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w34 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DNAG_MYCTU DNAG_MYCTU]] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.	+	[https://www.uniprot.org/uniprot/DNAG_MYCTU DNAG_MYCTU] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5w34" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5w34" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Myctu~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hou, C~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: ~~Tsodikov, O V~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Dna binding~~]]	+	[[Category: Hou C]]
-	[[Category: ~~Dna replication]]~~	+	[[Category: Tsodikov OV]]
-	~~[[Category: Replisome]]~~	+
-	~~[[Category: Toprim fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:38, 18 April 2018

OCA — Wed, 18 Apr 2018 06:38:59 GMT

←Older revision		Revision as of 06:38, 18 April 2018
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	<StructureSection load='5w34' size='340' side='right' caption='[[5w34]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='5w34' size='340' side='right' caption='[[5w34]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w34]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W34 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W34 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w34]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W34 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W34 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w33\|5w33]], [[5w35\|5w35]], [[5w36\|5w36]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w33\|5w33]], [[5w35\|5w35]], [[5w36\|5w36]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaG, Rv2343c, MTCY98.12c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w34 OCA], [http://pdbe.org/5w34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w34 RCSB], [http://www.ebi.ac.uk/pdbsum/5w34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w34 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w34 OCA], [http://pdbe.org/5w34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w34 RCSB], [http://www.ebi.ac.uk/pdbsum/5w34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w34 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Myctu]]
	[[Category: Hou, C]]		[[Category: Hou, C]]
	[[Category: Tsodikov, O V]]		[[Category: Tsodikov, O V]]

OCA at 06:31, 28 March 2018

OCA — Wed, 28 Mar 2018 06:31:45 GMT

←Older revision		Revision as of 06:31, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5w34 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with double-stranded DNA GACCGGAAGTGG==
		+	<StructureSection load='5w34' size='340' side='right' caption='[[5w34]], [[Resolution\|resolution]] 2.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5w34]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W34 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W34 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w33\|5w33]], [[5w35\|5w35]], [[5w36\|5w36]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w34 OCA], [http://pdbe.org/5w34 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w34 RCSB], [http://www.ebi.ac.uk/pdbsum/5w34 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w34 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNAG_MYCTU DNAG_MYCTU]] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial primase DnaG is an essential nucleic acid polymerase that generates primers for replication of chromosomal DNA. The mechanism of DnaG remains unclear due to the paucity of structural information on DnaG in complexes with other replisome components. Here we report the first crystal structures of noncovalent DnaG-DNA complexes, obtained with the RNA polymerase domain of Mycobacterium tuberculosis DnaG and various DNA ligands. One structure, obtained with ds DNA, reveals interactions with DnaG as it slides on ds DNA and suggests how DnaG binds template for primer synthesis. In another structure, DNA in the active site of DnaG mimics the primer, providing insight into mechanisms for the nucleotide transfer and DNA translocation. In conjunction with the recent cryo-EM structure of the bacteriophage T7 replisome, this study yields a model for primer elongation and hand-off to DNA polymerase.

-	~~Authors~~:	+	Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events.,Hou C, Biswas T, Tsodikov OV Biochemistry. 2018 Mar 23. doi: 10.1021/acs.biochem.8b00036. PMID:29558114<ref>PMID:29558114</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5w34" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hou, C]]
		+	[[Category: Tsodikov, O V]]
		+	[[Category: Dna binding]]
		+	[[Category: Dna replication]]
		+	[[Category: Replisome]]
		+	[[Category: Toprim fold]]
		+	[[Category: Transferase]]

OCA: Protected "5w34" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:23:53 GMT

Protected "5w34" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:23, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5w34 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 14:23:52 GMT

New page: '''Unreleased structure''' The entry 5w34 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5w34 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]