5w39 - Revision history

OCA at 14:06, 4 October 2023

OCA — Wed, 04 Oct 2023 14:06:11 GMT

←Older revision		Revision as of 14:06, 4 October 2023
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	==Crystal structure of mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure==		==Crystal structure of mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure==
-	<StructureSection load='5w39' size='340' side='right' caption='[[5w39]], [[Resolution\|resolution]] 2.48Å' scene=''>	+	<StructureSection load='5w39' size='340' side='right'caption='[[5w39]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w39]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W39 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W39 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w39]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W39 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9UM:3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione'>9UM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.48Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w17\|5w17]], [[5w37\|5w37]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9UM:3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione'>9UM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A0L11_08945, A0M64_02260, A0M70_03345, AD53_02580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w39 OCA], [https://pdbe.org/5w39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w39 RCSB], [https://www.ebi.ac.uk/pdbsum/5w39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w39 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w39 OCA], [~~http~~://pdbe.org/5w39 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w39 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w39 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w39 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0PB90_CAMJE Q0PB90_CAMJE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
-	[[Category: ~~Huber, T R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Snow, C D~~]]	+	[[Category: Huber TR]]
-	[[Category: ~~Nanoporous]]~~	+	[[Category: Snow CD]]
-	~~[[Category: Nanotechnology]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:07, 31 January 2018

OCA — Wed, 31 Jan 2018 07:07:34 GMT

←Older revision		Revision as of 07:07, 31 January 2018
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	<StructureSection load='5w39' size='340' side='right' caption='[[5w39]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='5w39' size='340' side='right' caption='[[5w39]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w39]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W39 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W39 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w39]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W39 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W39 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9UM:3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione'>9UM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9UM:3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione'>9UM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w17\|5w17]], [[5w37\|5w37]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w17\|5w17]], [[5w37\|5w37]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A0L11_08945, A0M64_02260, A0M70_03345, AD53_02580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w39 OCA], [http://pdbe.org/5w39 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w39 RCSB], [http://www.ebi.ac.uk/pdbsum/5w39 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w39 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w39 OCA], [http://pdbe.org/5w39 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w39 RCSB], [http://www.ebi.ac.uk/pdbsum/5w39 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w39 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Huber, T R]]		[[Category: Huber, T R]]
	[[Category: Snow, C D]]		[[Category: Snow, C D]]

OCA at 05:46, 3 January 2018

OCA — Wed, 03 Jan 2018 05:46:46 GMT

←Older revision		Revision as of 05:46, 3 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5w39 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure==
		+	<StructureSection load='5w39' size='340' side='right' caption='[[5w39]], [[Resolution\|resolution]] 2.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5w39]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W39 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W39 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9UM:3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione'>9UM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w17\|5w17]], [[5w37\|5w37]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w39 OCA], [http://pdbe.org/5w39 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w39 RCSB], [http://www.ebi.ac.uk/pdbsum/5w39 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w39 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.

-	~~Authors:~~ Huber, ~~T.R.~~, ~~Snow~~, C.D.	+	Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.,Huber TR, McPherson EC, Keating CE, Snow CD Bioconjug Chem. 2017 Dec 20. doi: 10.1021/acs.bioconjchem.7b00668. PMID:29232505<ref>PMID:29232505</ref>

-	~~Description: Crystal structure~~ of ~~mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Huber, T.R]]	+	<div class="pdbe-citations 5w39" style="background-color:#fffaf0;"></div>
-	[[Category: Snow, C.D]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huber, T R]]
		+	[[Category: Snow, C D]]
		+	[[Category: Nanoporous]]
		+	[[Category: Nanotechnology]]
		+	[[Category: Unknown function]]

OCA: Protected "5w39" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:24:17 GMT

Protected "5w39" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:24, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5w39 is ON HOLD until Paper Publication Authors: Huber, T.R., Snow, C.D. Description: Crystal structure of mutant CJ YCEI protein (CJ-N182C) with m...

OCA — Wed, 27 Sep 2017 14:24:16 GMT

New page: '''Unreleased structure''' The entry 5w39 is ON HOLD until Paper Publication Authors: Huber, T.R., Snow, C.D. Description: Crystal structure of mutant CJ YCEI protein (CJ-N182C) with m...

New page

'''Unreleased structure'''

The entry 5w39 is ON HOLD until Paper Publication

Authors: Huber, T.R., Snow, C.D.

Description: Crystal structure of mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure
[[Category: Unreleased Structures]]
[[Category: Huber, T.R]]
[[Category: Snow, C.D]]