5was - Revision history

OCA at 14:09, 4 October 2023

OCA — Wed, 04 Oct 2023 14:09:47 GMT

←Older revision		Revision as of 14:09, 4 October 2023
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	==Corynebacterium glutamicum Hydrolyzed Homoserine kinase==		==Corynebacterium glutamicum Hydrolyzed Homoserine kinase==
-	<StructureSection load='5was' size='340' side='right' caption='[[5was]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5was' size='340' side='right'caption='[[5was]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5was]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WAS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5was]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WAS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.799Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>thrB, Cgl1184, cg1338 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1718~~ "~~Micrococcus glutamicus~~" ~~Kinoshita et al. 1958])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Homoserine_kinase Homoserine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.39 2.7.1.39]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5was OCA], [https://pdbe.org/5was PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5was RCSB], [https://www.ebi.ac.uk/pdbsum/5was PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5was ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5was OCA], [~~http~~://pdbe.org/5was PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5was RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5was PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5was ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KHSE_CORGL KHSE_CORGL]] Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.	+	[https://www.uniprot.org/uniprot/KHSE_CORGL KHSE_CORGL] Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
-	[[Category: Homoserine kinase]]
-	[[Category: Petit, C]]
-	[[Category: Ronning, D R]]
	[[Category: Corynebacterium glutamicum]]		[[Category: Corynebacterium glutamicum]]
-	[[Category: ~~L-homoserine~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~L-threonine~~]]	+	[[Category: Petit C]]
-	[[Category: ~~Magnesium]]~~	+	[[Category: Ronning DR]]
-	~~[[Category: Transferase~~]]	+

OCA at 07:07, 31 October 2018

OCA — Wed, 31 Oct 2018 07:07:37 GMT

←Older revision		Revision as of 07:07, 31 October 2018
Line 3:		Line 3:
	<StructureSection load='5was' size='340' side='right' caption='[[5was]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5was' size='340' side='right' caption='[[5was]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5was]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5was]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WAS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thrB, Cgl1184, cg1338 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_kinase Homoserine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.39 2.7.1.39] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_kinase Homoserine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.39 2.7.1.39] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5was OCA], [http://pdbe.org/5was PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5was RCSB], [http://www.ebi.ac.uk/pdbsum/5was PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5was ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5was OCA], [http://pdbe.org/5was PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5was RCSB], [http://www.ebi.ac.uk/pdbsum/5was PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5was ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KHSE_CORGL KHSE_CORGL]] Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.		[[http://www.uniprot.org/uniprot/KHSE_CORGL KHSE_CORGL]] Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	l-Threonine is an important supplement in the food industry. It is currently produced through fermentation of Escherichia coli but requires additional purification steps to remove E. coli endotoxin. To avoid these steps, it is desirable to use Corynebacterium glutamicum, a microorganism generally regarded as safe. Engineering of C. glutamicum to increase production of l-threonine has mainly focused on gene regulation as well as l-threonine export or carbon flux depletion. In this study, we focus on the negative feedback inhibition produced by l-threonine on the enzyme homoserine kinase (ThrB). Although l-threonine binds to allosteric sites of aspartate kinase (LysC) and homoserine dehydrogenase (Hom), serving as a noncompetitive inhibitor, it acts as a competitive inhibitor on ThrB. This is problematic when attempting to engineer enzymes that are nonresponsive to increasing cellular concentrations of l-threonine. Using primary structure alignment as well as analysis of the Methanocaldococcus jannaschii ThrB (MjaThrB) active site in complex with l-threonine (inhibitor of ThrB) and l-homoserine (substrate of ThrB), a conserved active-site alanine residue (A20) in C. glutamicum ThrB (CglThrB) was predicted to be important for differential interactions with l-threonine and l-homoserine. Through site-directed mutagenesis, we show that one variant of C. glutamicum ThrB, CglThrB-A20G, retains wild-type enzymatic activity, with dramatically decreased feedback inhibition by l-threonine. Additionally, by solving the first Corynebacterium X-ray crystal structure of homoserine kinase, we can confirm that the changes in l-threonine affinity to the CglThrB-A20G active site derive from loss of van der Waals interactions.
		+
		+	Reduction of Feedback Inhibition in Homoserine Kinase (ThrB) of Corynebacterium glutamicum Enhances l-Threonine Biosynthesis.,Petit C, Kim Y, Lee SK, Brown J, Larsen E, Ronning DR, Suh JW, Kang CM ACS Omega. 2018 Jan 31;3(1):1178-1186. doi: 10.1021/acsomega.7b01597. Epub 2018, Jan 29. PMID:30023797<ref>PMID:30023797</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5was" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
	[[Category: Homoserine kinase]]		[[Category: Homoserine kinase]]
	[[Category: Petit, C]]		[[Category: Petit, C]]

OCA at 06:14, 7 February 2018

OCA — Wed, 07 Feb 2018 06:14:11 GMT

←Older revision		Revision as of 06:14, 7 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5was is ~~ON HOLD~~	+	==Corynebacterium glutamicum Hydrolyzed Homoserine kinase==
-		+	<StructureSection load='5was' size='340' side='right' caption='[[5was]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~Authors~~: ~~Petit~~, C., ~~Ronning~~, D.R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5was]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WAS FirstGlance]. <br>
-	~~Description: Corynebacterium glutamicum Hydrolyzed Homoserine kinase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_kinase Homoserine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.39 2.7.1.39] </span></td></tr>
-	[[Category: ~~Ronning, D.R~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5was OCA], [http://pdbe.org/5was PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5was RCSB], [http://www.ebi.ac.uk/pdbsum/5was PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5was ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KHSE_CORGL KHSE_CORGL]] Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homoserine kinase]]
	[[Category: Petit, C]]		[[Category: Petit, C]]
		+	[[Category: Ronning, D R]]
		+	[[Category: Corynebacterium glutamicum]]
		+	[[Category: L-homoserine]]
		+	[[Category: L-threonine]]
		+	[[Category: Magnesium]]
		+	[[Category: Transferase]]

OCA: Protected "5was" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:38:01 GMT

Protected "5was" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:38, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5was is ON HOLD Authors: Petit, C., Ronning, D.R. Description: Corynebacterium glutamicum Hydrolyzed Homoserine kinase [[Category: Unreleased Struc...

OCA — Wed, 27 Sep 2017 14:37:59 GMT

New page: '''Unreleased structure''' The entry 5was is ON HOLD Authors: Petit, C., Ronning, D.R. Description: Corynebacterium glutamicum Hydrolyzed Homoserine kinase [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 5was is ON HOLD

Authors: Petit, C., Ronning, D.R.

Description: Corynebacterium glutamicum Hydrolyzed Homoserine kinase
[[Category: Unreleased Structures]]
[[Category: Ronning, D.R]]
[[Category: Petit, C]]