5wco - Revision history

OCA at 17:33, 11 December 2019

OCA — Wed, 11 Dec 2019 17:33:40 GMT

←Older revision		Revision as of 17:33, 11 December 2019
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	==Matrix Protein (M1) of Infectious Salmon Anaemia Virus==		==Matrix Protein (M1) of Infectious Salmon Anaemia Virus==
-	<StructureSection load='5wco' size='340' side='right' caption='[[5wco]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5wco' size='340' side='right'caption='[[5wco]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wco]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Isav Isav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wco]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Isav Isav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCO FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Isav]]		[[Category: Isav]]
		+	[[Category: Large Structures]]
	[[Category: Betancourt, M A]]		[[Category: Betancourt, M A]]
	[[Category: Fan, Y]]		[[Category: Fan, Y]]

OCA at 20:18, 15 November 2017

OCA — Wed, 15 Nov 2017 20:18:59 GMT

←Older revision		Revision as of 20:18, 15 November 2017
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	<StructureSection load='5wco' size='340' side='right' caption='[[5wco]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5wco' size='340' side='right' caption='[[5wco]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wco]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wco]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Isav Isav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wco OCA], [http://pdbe.org/5wco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wco RCSB], [http://www.ebi.ac.uk/pdbsum/5wco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wco ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55987 ISAV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wco OCA], [http://pdbe.org/5wco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wco RCSB], [http://www.ebi.ac.uk/pdbsum/5wco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wco ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Isav]]
	[[Category: Betancourt, M A]]		[[Category: Betancourt, M A]]
	[[Category: Fan, Y]]		[[Category: Fan, Y]]
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	[[Category: Zheng, W]]		[[Category: Zheng, W]]
	[[Category: Helical]]		[[Category: Helical]]
-	[[Category: Isav]]
	[[Category: Matrix protein]]		[[Category: Matrix protein]]
	[[Category: Orthomyxovirus]]		[[Category: Orthomyxovirus]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA: Protected "5wco" [edit=sysop:move=sysop]

OCA — Thu, 17 Aug 2017 05:55:46 GMT

Protected "5wco" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 17 August 2017

OCA: New page: ==Matrix Protein (M1) of Infectious Salmon Anaemia Virus== == Str...

OCA — Thu, 17 Aug 2017 05:55:44 GMT

New page: ==Matrix Protein (M1) of Infectious Salmon Anaemia Virus== <StructureSection load='5wco' size='340' side='right' caption='5wco, resolution 2.60Å' scene=''> == Str...

New page

==Matrix Protein (M1) of Infectious Salmon Anaemia Virus==
<StructureSection load='5wco' size='340' side='right' caption='[[5wco]], [[Resolution|resolution]] 2.60Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wco]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WCO FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wco OCA], [http://pdbe.org/5wco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wco RCSB], [http://www.ebi.ac.uk/pdbsum/5wco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wco ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many enveloped viruses encode a matrix protein. In the influenza A virus, the matrix protein M1 polymerizes into a rigid protein layer underneath the viral envelope to help enforce the shape and structural integrity of intact viruses. The influenza virus M1 is also known to mediate virus budding as well as the nuclear export of the viral nucleocapsids and their subsequent packaging into nascent viral particles. Despite extensive studies on the influenza A virus M1 (FLUA-M1), only crystal structures of its N-terminal domain are available. Here we report the crystal structure of the full-length M1 from another orthomyxovirus that infects fish, the infectious salmon anemia virus (ISAV). The structure of ISAV-M1 assumes the shape of an elbow, with its N domain closely resembling that of the FLUA-M1. The C domain, which is connected to the N domain through a flexible linker, is made of four alpha-helices packed as a tight bundle. In the crystal, ISAV-M1 monomers form infinite 2D arrays with a network of interactions involving both the N and C domains. Results from liposome flotation assays indicated that ISAV-M1 binds membrane via electrostatic interactions that are primarily mediated by a positively charged surface loop from the N domain. Cryoelectron tomography reconstruction of intact ISA virions identified a matrix protein layer adjacent to the inner leaflet of the viral membrane. The physical dimensions of the virion-associated matrix layer are consistent with the 2D ISAV-M1 crystal lattice, suggesting that the crystal lattice is a valid model for studying M1-M1, M1-membrane, and M1-RNP interactions in the virion.

Crystal structure of an orthomyxovirus matrix protein reveals mechanisms for self-polymerization and membrane association.,Zhang W, Zheng W, Toh Y, Betancourt-Solis MA, Tu J, Fan Y, Vakharia VN, Liu J, McNew JA, Jin M, Tao YJ Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8550-8555. doi:, 10.1073/pnas.1701747114. Epub 2017 Jul 24. PMID:28739952<ref>PMID:28739952</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wco" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Betancourt, M A]]
[[Category: Fan, Y]]
[[Category: Jin, M]]
[[Category: Liu, J]]
[[Category: McNew, J A]]
[[Category: Tao, Y J]]
[[Category: Toh, Y]]
[[Category: Tu, J]]
[[Category: Vakharia, V]]
[[Category: Zhang, W]]
[[Category: Zheng, W]]
[[Category: Helical]]
[[Category: Isav]]
[[Category: Matrix protein]]
[[Category: Orthomyxovirus]]
[[Category: Viral protein]]