5weo - Revision history

OCA at 22:23, 10 April 2020

OCA — Fri, 10 Apr 2020 22:23:10 GMT

←Older revision		Revision as of 22:23, 10 April 2020
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	<SX load='5weo' size='340' side='right' viewer='molstar' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>		<SX load='5weo' size='340' side='right' viewer='molstar' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WEO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WEO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYZ:CYCLOTHIAZIDE'>CYZ</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYZ:CYCLOTHIAZIDE'>CYZ</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cacng2, Stg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cacng2, Stg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weo OCA], [http://pdbe.org/5weo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weo RCSB], [http://www.ebi.ac.uk/pdbsum/5weo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weo OCA], [http://pdbe.org/5weo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weo RCSB], [http://www.ebi.ac.uk/pdbsum/5weo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:58, 6 March 2020

OCA — Fri, 06 Mar 2020 19:58:02 GMT

←Older revision		Revision as of 19:58, 6 March 2020
Line 1:		Line 1:

	==Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin==		==Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin==
-	<~~StructureSection~~ load='5weo' size='340' side='right'caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>	+	<SX load='5weo' size='340' side='right' viewer='molstar' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]

OCA at 17:01, 20 November 2019

OCA — Wed, 20 Nov 2019 17:01:24 GMT

←Older revision		Revision as of 17:01, 20 November 2019
Line 1:		Line 1:

	==Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin==		==Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin==
-	<StructureSection load='5weo' size='340' side='right' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>	+	<StructureSection load='5weo' size='340' side='right'caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Frank, J]]		[[Category: Frank, J]]

OCA at 09:32, 18 July 2018

OCA — Wed, 18 Jul 2018 09:32:33 GMT

←Older revision		Revision as of 09:32, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='5weo' size='340' side='right' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='5weo' size='340' side='right' caption='[[5weo]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYZ:CYCLOTHIAZIDE'>CYZ</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYZ:CYCLOTHIAZIDE'>CYZ</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cacng2, Stg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weo OCA], [http://pdbe.org/5weo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weo RCSB], [http://www.ebi.ac.uk/pdbsum/5weo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weo OCA], [http://pdbe.org/5weo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weo RCSB], [http://www.ebi.ac.uk/pdbsum/5weo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weo ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Frank, J]]		[[Category: Frank, J]]
	[[Category: Grassucci, R A]]		[[Category: Grassucci, R A]]

OCA: Protected "5weo" [edit=sysop:move=sysop]

OCA — Fri, 04 Aug 2017 04:09:33 GMT

Protected "5weo" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:09, 4 August 2017

OCA: New page: ==Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin== == Structural highlights ==
<table><tr><td colspan='2'>[[5weo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYZ:CYCLOTHIAZIDE'>CYZ</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5weo OCA], [http://pdbe.org/5weo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5weo RCSB], [http://www.ebi.ac.uk/pdbsum/5weo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5weo ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/GRIA2_RAT GRIA2_RAT]] Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.<ref>PMID:9351977</ref> <ref>PMID:19265014</ref> <ref>PMID:21172611</ref> <ref>PMID:12501192</ref> <ref>PMID:12015593</ref> <ref>PMID:12872125</ref> <ref>PMID:12730367</ref> <ref>PMID:16192394</ref> <ref>PMID:15591246</ref> <ref>PMID:17018279</ref> <ref>PMID:16483599</ref> <ref>PMID:19946266</ref> <ref>PMID:21317873</ref> <ref>PMID:21846932</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
AMPA-subtype ionotropic glutamate receptors mediate fast excitatory neurotransmission throughout the central nervous system. Gated by the neurotransmitter glutamate, AMPA receptors are critical for synaptic strength and dysregulation of AMPA receptor-mediated signalling is linked to numerous neurological diseases. Here, we use cryo-electron microscopy to solve the structures of AMPA receptor-auxiliary subunit complexes in the apo, antagonist and agonist-bound states and elucidate the iris-like mechanism of ion channel opening. The ion channel selectivity filter is formed by the extended portions of the re-entrant M2 loops, while the helical portions of M2 contribute to extensive hydrophobic interfaces between AMPA receptor subunits in the ion channel. We show how the permeation pathway changes upon channel opening and identify conformational changes throughout the entire AMPA receptor that accompany activation and desensitization. Our findings provide a framework for understanding gating across the family of ionotropic glutamate receptors and the role of AMPA receptors in excitatory neurotransmission.

Channel opening and gating mechanism in AMPA-subtype glutamate receptors.,Twomey EC, Yelshanskaya MV, Grassucci RA, Frank J, Sobolevsky AI Nature. 2017 Jul 24. doi: 10.1038/nature23479. PMID:28737760<ref>PMID:28737760</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5weo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Frank, J]]
[[Category: Grassucci, R A]]
[[Category: Sobolevsky, A I]]
[[Category: Twomey, E C]]
[[Category: Yelshanskaya, M V]]
[[Category: Ion channel]]
[[Category: Transport protein]]