5wft - Revision history

OCA at 08:29, 8 January 2020

OCA — Wed, 08 Jan 2020 08:29:58 GMT

←Older revision		Revision as of 08:29, 8 January 2020
Line 1:		Line 1:

	==PelB 319-436 from Pseudomonas aeruginosa PAO1==		==PelB 319-436 from Pseudomonas aeruginosa PAO1==
-	<StructureSection load='5wft' size='340' side='right' caption='[[5wft]], [[Resolution\|resolution]] 2.82Å' scene=''>	+	<StructureSection load='5wft' size='340' side='right'caption='[[5wft]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WFT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WFT FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Howell, P L]]		[[Category: Howell, P L]]

OCA at 07:35, 13 December 2017

OCA — Wed, 13 Dec 2017 07:35:28 GMT

←Older revision		Revision as of 07:35, 13 December 2017
Line 3:		Line 3:
	<StructureSection load='5wft' size='340' side='right' caption='[[5wft]], [[Resolution\|resolution]] 2.82Å' scene=''>		<StructureSection load='5wft' size='340' side='right' caption='[[5wft]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WFT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WFT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pelB, PA3063 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wft OCA], [http://pdbe.org/5wft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wft RCSB], [http://www.ebi.ac.uk/pdbsum/5wft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wft ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wft OCA], [http://pdbe.org/5wft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wft RCSB], [http://www.ebi.ac.uk/pdbsum/5wft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wft ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Howell, P L]]		[[Category: Howell, P L]]
	[[Category: Marmont, L S]]		[[Category: Marmont, L S]]
	[[Category: Structural protein]]		[[Category: Structural protein]]
	[[Category: Tetratricopeptide repeat]]		[[Category: Tetratricopeptide repeat]]

OCA at 07:04, 18 October 2017

OCA — Wed, 18 Oct 2017 07:04:20 GMT

←Older revision		Revision as of 07:04, 18 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wft is ~~ON HOLD until Paper~~ Publication	+	==PelB 319-436 from Pseudomonas aeruginosa PAO1==
		+	<StructureSection load='5wft' size='340' side='right' caption='[[5wft]], [[Resolution\|resolution]] 2.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WFT FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wft OCA], [http://pdbe.org/5wft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wft RCSB], [http://www.ebi.ac.uk/pdbsum/5wft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wft ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Pel polysaccharide (PEL) is synthesized by the opportunistic pathogen Pseudomonas aeruginosa and is an important biofilm constituent critical for bacterial virulence and persistence. PEL is a cationic polymer that promotes cell-cell interactions within the biofilm matrix through electrostatic interactions with extracellular DNA (eDNA). Translocation of PEL across the outer membrane is proposed to occur via PelB, a membrane-embedded porin with a large periplasmic domain predicted to contain 19 tetratricopeptide repeats (TPRs). TPR-containing domains are typically involved in protein-protein interactions, and we therefore sought to determine whether PelB serves as a periplasmic scaffold that recruits other components of the PEL secretion apparatus. In this study, we show that the TPR domain of PelB interacts with PelA, an enzyme with PEL deacetylase and hydrolase activities. Structure determination of PelB TPRs 8-11 enabled us to design systematic deletions of individual TPRs and revealed that repeats 9-13, which are required for the cellular localization of PelA with PelB are also essential for PEL-dependent biofilm formation. Co-purification experiments indicated that the interaction between PelA and PelB is direct, and that the deacetylase activity of PelA increases and its hydrolase activity decreases when these proteins interact. Combined, our results indicate that the TPR-containing domain of PelB localizes PelA to the PEL secretion apparatus within the periplasm, and that this may allow for efficient deacetylation of PEL before to its export from the cell.

-	~~Authors~~:	+	PelA and PelB form a modification and secretion complex essential for Pel polysaccharide-dependent biofilm formation in Pseudomonas aeruginosa.,Marmont LS, Whitfield GB, Rich JD, Yip P, Giesbrecht LB, Stremick CA, Whitney JC, Parsek MR, Harrison JJ, Howell PL J Biol Chem. 2017 Sep 27. pii: jbc.M117.812842. doi: 10.1074/jbc.M117.812842. PMID:28972168<ref>PMID:28972168</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5wft" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Howell, P L]]
		+	[[Category: Marmont, L S]]
		+	[[Category: Structural protein]]
		+	[[Category: Tetratricopeptide repeat]]

OCA: Protected "5wft" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:47:05 GMT

Protected "5wft" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:47, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wft is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 14:47:04 GMT

New page: '''Unreleased structure''' The entry 5wft is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wft is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]