5wgc - Revision history

OCA at 14:12, 4 October 2023

OCA — Wed, 04 Oct 2023 14:12:23 GMT

←Older revision		Revision as of 14:12, 4 October 2023
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	<StructureSection load='5wgc' size='340' side='right'caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5wgc' size='340' side='right'caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1799 As 4.1799~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WGC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=AFY:(3~~-{[~~2-({N-~~[~~(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl~~]~~-beta-alanyl}amino)ethyl~~]~~sulfanyl}oxetan-3-yl)acetic+acid'>AFY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=42Y:O-PROPANOYL-L-SERINE'>42Y</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=42Y:O-PROPANOYL-L-SERINE'>42Y</scene>, <scene name='pdbligand=AFY:(3-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}oxetan-3-yl)acetic+acid'>AFY</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>~~[[~~Gene\|Gene:~~]]~~</b~~></td>~~<td class="sblockDat">dpsC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1950 AS 4.1799])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wgc OCA], [https://pdbe.org/5wgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wgc RCSB], [https://www.ebi.ac.uk/pdbsum/5wgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wgc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wgc OCA], [~~http~~://pdbe.org/5wgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wgc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q54816_STRPE Q54816_STRPE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1799]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ellis, B D~~]]	+	[[Category: Streptomyces peucetius]]
-	[[Category: ~~Milligan, J C~~]]	+	[[Category: Ellis BD]]
-	[[Category: ~~Tsai, S C~~]]	+	[[Category: Milligan JC]]
-	[[Category: ~~Vanderwal, C D~~]]	+	[[Category: Tsai SC]]
-	[[Category: ~~White, A R~~]]	+	[[Category: Vanderwal CD]]
-	[[Category: ~~Enzyme-probe complex]]~~	+	[[Category: White AR]]
-	~~[[Category: Transferase~~]]	+

OCA at 09:50, 1 January 2020

OCA — Wed, 01 Jan 2020 09:50:36 GMT

←Older revision		Revision as of 09:50, 1 January 2020
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	==propionyl-DpsC in complex with oxetane-bearing probe==		==propionyl-DpsC in complex with oxetane-bearing probe==
-	<StructureSection load='5wgc' size='340' side='right' caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5wgc' size='340' side='right'caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1799 As 4.1799]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1799 As 4.1799]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WGC FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1799]]		[[Category: As 4 1799]]
		+	[[Category: Large Structures]]
	[[Category: Ellis, B D]]		[[Category: Ellis, B D]]
	[[Category: Milligan, J C]]		[[Category: Milligan, J C]]

OCA at 08:26, 2 May 2018

OCA — Wed, 02 May 2018 08:26:17 GMT

←Older revision		Revision as of 08:26, 2 May 2018
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	<StructureSection load='5wgc' size='340' side='right' caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5wgc' size='340' side='right' caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1799 As 4.1799]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WGC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFY:(3-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}oxetan-3-yl)acetic+acid'>AFY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFY:(3-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}oxetan-3-yl)acetic+acid'>AFY</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=42Y:O-PROPANOYL-L-SERINE'>42Y</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=42Y:O-PROPANOYL-L-SERINE'>42Y</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dpsC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1950 AS 4.1799])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wgc OCA], [http://pdbe.org/5wgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wgc RCSB], [http://www.ebi.ac.uk/pdbsum/5wgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wgc OCA], [http://pdbe.org/5wgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wgc RCSB], [http://www.ebi.ac.uk/pdbsum/5wgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wgc ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1799]]
	[[Category: Ellis, B D]]		[[Category: Ellis, B D]]
	[[Category: Milligan, J C]]		[[Category: Milligan, J C]]

OCA at 05:39, 18 April 2018

OCA — Wed, 18 Apr 2018 05:39:26 GMT

←Older revision		Revision as of 05:39, 18 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wgc is ~~ON HOLD until Paper~~ Publication	+	==propionyl-DpsC in complex with oxetane-bearing probe==
		+	<StructureSection load='5wgc' size='340' side='right' caption='[[5wgc]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wgc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WGC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFY:(3-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}oxetan-3-yl)acetic+acid'>AFY</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=42Y:O-PROPANOYL-L-SERINE'>42Y</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wgc OCA], [http://pdbe.org/5wgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wgc RCSB], [http://www.ebi.ac.uk/pdbsum/5wgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wgc ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyketides are a large class of bioactive natural products with a wide range of structures and functions. Polyketides are biosynthesized by large, multidomain enzyme complexes termed polyketide synthases (PKSs). One of the primary challenges when studying PKSs is the high reactivity of their poly-beta-ketone substrates. This has hampered structural and mechanistic characterization of PKS-polyketide complexes, and, as a result, little is known about how PKSs position the unstable substrates for proper catalysis while displaying high levels of regio- and stereospecificity. As a first step toward a general plan to use oxetanes as carbonyl isosteres to broadly interrogate PKS chemistry, we describe the development and application of an oxetane-based PKS substrate mimic. This enabled the first structural determination of the acyl-enzyme intermediate of a ketosynthase (KS) in complex with an inert extender unit mimic. The crystal structure, in combination with molecular dynamics simulations, led to a proposed mechanism for the unique activity of DpsC, the priming ketosynthase for daunorubicin biosynthesis. The successful application of an oxetane-based polyketide mimic suggests that this novel class of probes could have wide-ranging applications to the greater biosynthetic community interested in the mechanistic enzymology of iterative PKSs.

-	~~Authors~~:	+	An Oxetane-Based Polyketide Surrogate To Probe Substrate Binding in a Polyketide Synthase.,Ellis BD, Milligan JC, White AR, Duong V, Altman PX, Mohammed LY, Crump MP, Crosby J, Luo R, Vanderwal CD, Tsai SC J Am Chem Soc. 2018 Apr 10. doi: 10.1021/jacs.7b11793. PMID:29620883<ref>PMID:29620883</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5wgc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ellis, B D]]
		+	[[Category: Milligan, J C]]
		+	[[Category: Tsai, S C]]
		+	[[Category: Vanderwal, C D]]
		+	[[Category: White, A R]]
		+	[[Category: Enzyme-probe complex]]
		+	[[Category: Transferase]]

OCA: Protected "5wgc" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:48:07 GMT

Protected "5wgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:48, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wgc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 14:48:06 GMT

New page: '''Unreleased structure''' The entry 5wgc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wgc is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]