5whj - Revision history

OCA at 14:13, 4 October 2023

OCA — Wed, 04 Oct 2023 14:13:03 GMT

←Older revision		Revision as of 14:13, 4 October 2023
Line 1:		Line 1:

	==Crystal structure of Fab fragment of anti-FcRn antibody DX-2507==		==Crystal structure of Fab fragment of anti-FcRn antibody DX-2507==
-	<StructureSection load='5whj' size='340' side='right' caption='[[5whj]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5whj' size='340' side='right'caption='[[5whj]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5whj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WHJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5whj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WHJ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~IGL@ (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whj OCA], [~~http~~://pdbe.org/5whj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5whj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5whj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5whj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whj OCA], [https://pdbe.org/5whj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5whj RCSB], [https://www.ebi.ac.uk/pdbsum/5whj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5whj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/S6BAN1_HUMAN S6BAN1_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Edwards, T E~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fairman, J W~~]]	+	[[Category: Edwards TE]]
-	[[Category: ~~Kenniston, J A~~]]	+	[[Category: Fairman JW]]
-	[[Category: ~~Nixon, A E~~]]	+	[[Category: Kenniston JA]]
-	[[Category: ~~B2m]]~~	+	[[Category: Nixon AE]]
-	~~[[Category: Beta-2-microglobulin]]~~	+
-	~~[[Category: Fabricator]]~~	+
-	~~[[Category: Fcrn]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 07:56, 21 March 2018

OCA — Wed, 21 Mar 2018 07:56:14 GMT

←Older revision		Revision as of 07:56, 21 March 2018
Line 3:		Line 3:
	<StructureSection load='5whj' size='340' side='right' caption='[[5whj]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5whj' size='340' side='right' caption='[[5whj]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5whj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WHJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5whj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WHJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whj OCA], [http://pdbe.org/5whj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5whj RCSB], [http://www.ebi.ac.uk/pdbsum/5whj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5whj ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGL@ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whj OCA], [http://pdbe.org/5whj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5whj RCSB], [http://www.ebi.ac.uk/pdbsum/5whj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5whj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The neonatal Fc receptor FcRn plays a critical role in the trafficking of IgGs across tissue barriers and in retaining high circulating concentrations of both IgG and albumin. Although generally beneficial from an immunological perspective in maintaining IgG populations, FcRn can contribute to the pathogenesis of autoimmune disorders when an abnormal immune response targets normal biological components. We previously described a monoclonal antibody (DX-2507) that binds to FcRn with high affinity at both neutral and acidic pH, prevents the simultaneous binding of IgG, and reduces circulating IgG levels in preclinical animal models. Here, we report a 2.5 A resolution X-ray crystal structure of an FcRn-DX-2507 Fab complex, revealing a nearly complete overlap of the IgG-Fc binding site in FcRn by complementarity-determining regions in DX-2507. This overlap explains how DX-2507 blocks IgG binding to FcRn and thereby shortens IgG half-life by preventing IgGs from recycling back into circulation. Moreover, the complex structure explains how the DX-2507 interaction is pH-insensitive unlike normal Fc interactions and how serum albumin levels are unaffected by DX-2507 binding. These structural studies could inform antibody-based therapeutic approaches for limiting the effects of IgG-mediated autoimmune disease.
		+
		+	Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody.,Kenniston JA, Taylor BM, Conley GP, Cosic J, Kopacz KJ, Lindberg AP, Comeau SR, Atkins K, Bullen J, TenHoor C, Adelman BA, Sexton DJ, Edwards TE, Nixon AE J Biol Chem. 2017 Oct 20;292(42):17449-17460. doi: 10.1074/jbc.M117.807396. Epub , 2017 Sep 6. PMID:28878017<ref>PMID:28878017</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5whj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Edwards, T E]]		[[Category: Edwards, T E]]
	[[Category: Fairman, J W]]		[[Category: Fairman, J W]]

OCA: Protected "5whj" [edit=sysop:move=sysop]

OCA — Wed, 13 Sep 2017 09:51:21 GMT

Protected "5whj" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:51, 13 September 2017

OCA: New page: ==Crystal structure of Fab fragment of anti-FcRn antibody DX-2507== == Structural highlights ==
<table><tr><td colspan='2'>[[5whj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WHJ FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whj OCA], [http://pdbe.org/5whj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5whj RCSB], [http://www.ebi.ac.uk/pdbsum/5whj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5whj ProSAT]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Edwards, T E]]
[[Category: Fairman, J W]]
[[Category: Kenniston, J A]]
[[Category: Nixon, A E]]
[[Category: B2m]]
[[Category: Beta-2-microglobulin]]
[[Category: Fabricator]]
[[Category: Fcrn]]
[[Category: Immune system]]