5wjq - Revision history

OCA at 14:15, 4 October 2023

OCA — Wed, 04 Oct 2023 14:15:21 GMT

←Older revision		Revision as of 14:15, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5wjq' size='340' side='right'caption='[[5wjq]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='5wjq' size='340' side='right'caption='[[5wjq]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wjq]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wjq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WJQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=TRS:2-AMINO~~-2~~-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.794Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Znf568, chato, Zfp568 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjq OCA], [~~http~~://pdbe.org/5wjq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wjq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wjq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjq OCA], [https://pdbe.org/5wjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wjq RCSB], [https://www.ebi.ac.uk/pdbsum/5wjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ZN568_MOUSE ZN568_MOUSE]] Has transcriptional repression activity, partially through the recruitment of the corepressor TRIM28 but has also repression activity independently of this interaction (PubMed:22110054, PubMed:27658112). Essential during embryonic development, where it acts as direct repressor of IGF2-P0, placental-specific transcript of IGF2, in early development and regulates convergent extension movements required for axis elongation and tissue morphogenesis in all germ layers (PubMed:18701545, PubMed:22110054, PubMed:28522536). Also important for normal morphogenesis of extraembryonic tissues including the yolk sac, extraembryonic mesoderm and placenta (PubMed:18701545, PubMed:21094155). May enhance proliferation or maintenance of neural stem cells (PubMed:23071813).<ref>PMID:18701545</ref> <ref>PMID:21094155</ref> <ref>PMID:22110054</ref> <ref>PMID:23071813</ref> <ref>PMID:27658112</ref>	+	[https://www.uniprot.org/uniprot/ZN568_MOUSE ZN568_MOUSE] Has transcriptional repression activity, partially through the recruitment of the corepressor TRIM28 but has also repression activity independently of this interaction (PubMed:22110054, PubMed:27658112). Essential during embryonic development, where it acts as direct repressor of IGF2-P0, placental-specific transcript of IGF2, in early development and regulates convergent extension movements required for axis elongation and tissue morphogenesis in all germ layers (PubMed:18701545, PubMed:22110054, PubMed:28522536). Also important for normal morphogenesis of extraembryonic tissues including the yolk sac, extraembryonic mesoderm and placenta (PubMed:18701545, PubMed:21094155). May enhance proliferation or maintenance of neural stem cells (PubMed:23071813).<ref>PMID:18701545</ref> <ref>PMID:21094155</ref> <ref>PMID:22110054</ref> <ref>PMID:23071813</ref> <ref>PMID:27658112</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Cheng, X]]	+	[[Category: Cheng X]]
-	[[Category: Patel, A]]	+	[[Category: Patel A]]
-	~~[[Category: C2h2 type zinc finger]]~~	+
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Gene regulation]]~~	+
-	~~[[Category: Gene regulation-dna complex]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 09:51, 1 January 2020

OCA — Wed, 01 Jan 2020 09:51:58 GMT

←Older revision		Revision as of 09:51, 1 January 2020
Line 1:		Line 1:

	==mouseZFP568-ZnF2-11 in complex with DNA==		==mouseZFP568-ZnF2-11 in complex with DNA==
-	<StructureSection load='5wjq' size='340' side='right' caption='[[5wjq]], [[Resolution\|resolution]] 2.79Å' scene=''>	+	<StructureSection load='5wjq' size='340' side='right'caption='[[5wjq]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wjq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wjq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Znf568, chato, Zfp568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjq OCA], [http://pdbe.org/5wjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjq RCSB], [http://www.ebi.ac.uk/pdbsum/5wjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjq OCA], [http://pdbe.org/5wjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjq RCSB], [http://www.ebi.ac.uk/pdbsum/5wjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ZN568_MOUSE ZN568_MOUSE]] Has transcriptional repression activity, partially through the recruitment of the corepressor TRIM28 but has also repression activity independently of this interaction (PubMed:22110054, PubMed:27658112). Essential during embryonic development, where it acts as direct repressor of IGF2-P0, placental-specific transcript of IGF2, in early development and regulates convergent extension movements required for axis elongation and tissue morphogenesis in all germ layers (PubMed:18701545, PubMed:22110054, PubMed:28522536). Also important for normal morphogenesis of extraembryonic tissues including the yolk sac, extraembryonic mesoderm and placenta (PubMed:18701545, PubMed:21094155). May enhance proliferation or maintenance of neural stem cells (PubMed:23071813).<ref>PMID:18701545</ref> <ref>PMID:21094155</ref> <ref>PMID:22110054</ref> <ref>PMID:23071813</ref> <ref>PMID:27658112</ref>		[[http://www.uniprot.org/uniprot/ZN568_MOUSE ZN568_MOUSE]] Has transcriptional repression activity, partially through the recruitment of the corepressor TRIM28 but has also repression activity independently of this interaction (PubMed:22110054, PubMed:27658112). Essential during embryonic development, where it acts as direct repressor of IGF2-P0, placental-specific transcript of IGF2, in early development and regulates convergent extension movements required for axis elongation and tissue morphogenesis in all germ layers (PubMed:18701545, PubMed:22110054, PubMed:28522536). Also important for normal morphogenesis of extraembryonic tissues including the yolk sac, extraembryonic mesoderm and placenta (PubMed:18701545, PubMed:21094155). May enhance proliferation or maintenance of neural stem cells (PubMed:23071813).<ref>PMID:18701545</ref> <ref>PMID:21094155</ref> <ref>PMID:22110054</ref> <ref>PMID:23071813</ref> <ref>PMID:27658112</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tandem zinc finger (ZF) proteins are the largest and most rapidly diverging family of DNA-binding transcription regulators in mammals. ZFP568 represses a transcript of placental-specific insulin like growth factor 2 (Igf2-P0) in mice. ZFP568 binds a 24-base pair sequence-specific element upstream of Igf2-P0 via the eleven-ZF array. Both DNA and protein conformations deviate from the conventional one finger-three bases recognition, with individual ZFs contacting 2, 3, or 4 bases and recognizing thymine on the opposite strand. These interactions arise from a shortened minor groove caused by an AT-rich stretch, suggesting adaptability of ZF arrays to sequence variations. Despite conservation in mammals, mutations at Igf2 and ZFP568 reduce their binding affinity in chimpanzee and humans. Our studies provide important insights into the evolutionary and structural dynamics of ZF-DNA interactions that play a key role in mammalian development and evolution.
		+
		+	DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins.,Patel A, Yang P, Tinkham M, Pradhan M, Sun MA, Wang Y, Hoang D, Wolf G, Horton JR, Zhang X, Macfarlan T, Cheng X Cell. 2018 Mar 22;173(1):221-233.e12. doi: 10.1016/j.cell.2018.02.058. Epub 2018 , Mar 15. PMID:29551271<ref>PMID:29551271</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wjq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Cheng, X]]		[[Category: Cheng, X]]
	[[Category: Patel, A]]		[[Category: Patel, A]]

OCA at 05:02, 8 March 2018

OCA — Thu, 08 Mar 2018 05:02:46 GMT

←Older revision		Revision as of 05:02, 8 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wjq is ~~ON HOLD until Paper Publication~~	+	==mouseZFP568-ZnF2-11 in complex with DNA==
-		+	<StructureSection load='5wjq' size='340' side='right' caption='[[5wjq]], [[Resolution\|resolution]] 2.79Å' scene=''>
-	~~Authors~~: ~~Patel~~, A., ~~Cheng~~, X.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5wjq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJQ FirstGlance]. <br>
-	~~Description~~: ~~mouseZFP568~~-~~ZnF2-11 in complex with DNA~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjq OCA], [http://pdbe.org/5wjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjq RCSB], [http://www.ebi.ac.uk/pdbsum/5wjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ZN568_MOUSE ZN568_MOUSE]] Has transcriptional repression activity, partially through the recruitment of the corepressor TRIM28 but has also repression activity independently of this interaction (PubMed:22110054, PubMed:27658112). Essential during embryonic development, where it acts as direct repressor of IGF2-P0, placental-specific transcript of IGF2, in early development and regulates convergent extension movements required for axis elongation and tissue morphogenesis in all germ layers (PubMed:18701545, PubMed:22110054, PubMed:28522536). Also important for normal morphogenesis of extraembryonic tissues including the yolk sac, extraembryonic mesoderm and placenta (PubMed:18701545, PubMed:21094155). May enhance proliferation or maintenance of neural stem cells (PubMed:23071813).<ref>PMID:18701545</ref> <ref>PMID:21094155</ref> <ref>PMID:22110054</ref> <ref>PMID:23071813</ref> <ref>PMID:27658112</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Cheng, X]]		[[Category: Cheng, X]]
	[[Category: Patel, A]]		[[Category: Patel, A]]
		+	[[Category: C2h2 type zinc finger]]
		+	[[Category: Dna binding]]
		+	[[Category: Gene regulation]]
		+	[[Category: Gene regulation-dna complex]]
		+	[[Category: Transferase-dna complex]]

OCA: Protected "5wjq" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:54:01 GMT

Protected "5wjq" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:54, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wjq is ON HOLD until Paper Publication Authors: Patel, A., Cheng, X. Description: mouseZFP568-ZnF2-11 in complex with DNA [[Category: Unreleased S...

OCA — Wed, 27 Sep 2017 14:53:59 GMT

New page: '''Unreleased structure''' The entry 5wjq is ON HOLD until Paper Publication Authors: Patel, A., Cheng, X. Description: mouseZFP568-ZnF2-11 in complex with DNA [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 5wjq is ON HOLD until Paper Publication

Authors: Patel, A., Cheng, X.

Description: mouseZFP568-ZnF2-11 in complex with DNA
[[Category: Unreleased Structures]]
[[Category: Cheng, X]]
[[Category: Patel, A]]