5wjy - Revision history

OCA at 22:25, 10 April 2020

OCA — Fri, 10 Apr 2020 22:25:14 GMT

←Older revision		Revision as of 22:25, 10 April 2020
Line 3:		Line 3:
	<SX load='5wjy' size='340' side='right' viewer='molstar' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>		<SX load='5wjy' size='340' side='right' viewer='molstar' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WJY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WJY FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B4417_3365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B4417_3365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:58, 6 March 2020

OCA — Fri, 06 Mar 2020 19:58:50 GMT

←Older revision		Revision as of 19:58, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of B. subtilis flagellar filaments S285P==		==Cryo-EM structure of B. subtilis flagellar filaments S285P==
-	<~~StructureSection~~ load='5wjy' size='340' side='right'caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>	+	<SX load='5wjy' size='340' side='right' viewer='molstar' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>
Line 24:		Line 24:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 09:53, 1 January 2020

OCA — Wed, 01 Jan 2020 09:53:30 GMT

←Older revision		Revision as of 09:53, 1 January 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Cryo-EM structure of B. subtilis flagellar filaments S285P==		==Cryo-EM structure of B. subtilis flagellar filaments S285P==
-	<StructureSection load='5wjy' size='340' side='right' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>	+	<StructureSection load='5wjy' size='340' side='right'caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/A0A162QQD4_BACIU A0A162QQD4_BACIU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073]		[[http://www.uniprot.org/uniprot/A0A162QQD4_BACIU A0A162QQD4_BACIU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073]
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5wjy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5wjy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellin 3D structures\|Flagellin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Burrage, A M]]		[[Category: Burrage, A M]]
	[[Category: Egelman, E H]]		[[Category: Egelman, E H]]

OCA at 06:10, 1 November 2017

OCA — Wed, 01 Nov 2017 06:10:36 GMT

←Older revision		Revision as of 06:10, 1 November 2017
Line 3:		Line 3:
	<StructureSection load='5wjy' size='340' side='right' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>		<StructureSection load='5wjy' size='340' side='right' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B4417_3365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>
	</table>		</table>
	{{Large structure}}		{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/A0A162QQD4_BACIU A0A162QQD4_BACIU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073]		[[http://www.uniprot.org/uniprot/A0A162QQD4_BACIU A0A162QQD4_BACIU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa. Seven mutant flagellar filaments in B. subtilis and two in P. aeruginosa capture two different states of the filament. These reliable atomic models of both states reveal conserved molecular interactions in the interior of the filament among B. subtilis, P. aeruginosa and Salmonella enterica. Using the detailed information about the molecular interactions in two filament states, we successfully predict point mutations that shift the equilibrium between those two states. Further, we observe the dimerization of P. aeruginosa outer domains without any perturbation of the conserved interior of the filament. Our results give new insights into how the flagellin sequence has been "tuned" over evolution.Bacterial flagellar filaments are composed almost entirely of a single protein-flagellin-which can switch between different supercoiled states in a highly cooperative manner. Here the authors present near-atomic resolution cryo-EM structures of nine flagellar filaments, and begin to shed light on the molecular basis of filament switching.
		+
		+	A structural model of flagellar filament switching across multiple bacterial species.,Wang F, Burrage AM, Postel S, Clark RE, Orlova A, Sundberg EJ, Kearns DB, Egelman EH Nat Commun. 2017 Oct 16;8(1):960. doi: 10.1038/s41467-017-01075-5. PMID:29038601<ref>PMID:29038601</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wjy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Burrage, A M]]		[[Category: Burrage, A M]]
	[[Category: Egelman, E H]]		[[Category: Egelman, E H]]

OCA at 07:23, 25 October 2017

OCA — Wed, 25 Oct 2017 07:23:37 GMT

←Older revision		Revision as of 07:23, 25 October 2017
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	{{Large structure}}
-		+	==Cryo-EM structure of B. subtilis flagellar filaments S285P==
-	~~The entry~~ 5wjy is ~~ON HOLD until Paper Publication~~	+	<StructureSection load='5wjy' size='340' side='right' caption='[[5wjy]], [[Resolution\|resolution]] 4.50Å' scene=''>
-		+	== Structural highlights ==
-	~~Authors~~: ~~Wang~~, F., ~~Burrage~~, A.M., ~~Kearns, D~~.B., ~~Egelman, E~~.H.	+	<table><tr><td colspan='2'>[[5wjy]] is a 41 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJY FirstGlance]. <br>
-		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjy OCA], [http://pdbe.org/5wjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjy RCSB], [http://www.ebi.ac.uk/pdbsum/5wjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjy ProSAT]</span></td></tr>
-	~~Description: Cryo-EM~~ structure ~~of B. subtilis flagellar filaments S285P~~	+	</table>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	{{Large structure}}
-	[[Category: Burrage, A.M]]	+	== Function ==
-	[[Category: Egelman, E.H]]	+	[[http://www.uniprot.org/uniprot/A0A162QQD4_BACIU A0A162QQD4_BACIU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burrage, A M]]
		+	[[Category: Egelman, E H]]
		+	[[Category: Kearns, D B]]
	[[Category: Wang, F]]		[[Category: Wang, F]]
-	[[Category: ~~Kearns, D.B~~]]	+	[[Category: Bacteria flagella]]
		+	[[Category: Cryo-em]]
		+	[[Category: Helical polymer]]
		+	[[Category: Protein fibril]]

OCA: Protected "5wjy" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:54:23 GMT

Protected "5wjy" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:54, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wjy is ON HOLD until Paper Publication Authors: Wang, F., Burrage, A.M., Kearns, D.B., Egelman, E.H. Description: Cryo-EM structure of B. subtilis...

OCA — Wed, 27 Sep 2017 14:54:22 GMT

New page: '''Unreleased structure''' The entry 5wjy is ON HOLD until Paper Publication Authors: Wang, F., Burrage, A.M., Kearns, D.B., Egelman, E.H. Description: Cryo-EM structure of B. subtilis...

New page

'''Unreleased structure'''

The entry 5wjy is ON HOLD until Paper Publication

Authors: Wang, F., Burrage, A.M., Kearns, D.B., Egelman, E.H.

Description: Cryo-EM structure of B. subtilis flagellar filaments S285P
[[Category: Unreleased Structures]]
[[Category: Burrage, A.M]]
[[Category: Egelman, E.H]]
[[Category: Wang, F]]
[[Category: Kearns, D.B]]