5wk0 - Revision history

OCA at 14:15, 4 October 2023

OCA — Wed, 04 Oct 2023 14:15:26 GMT

←Older revision		Revision as of 14:15, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5wk0' size='340' side='right'caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>		<StructureSection load='5wk0' size='340' side='right'caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staphylococcus_sp.~~_hmsc055h04~~ Staphylococcus sp. ~~hmsc055h04~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_sp._HMSC055H04 Staphylococcus sp. HMSC055H04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NI:NICKEL+(II)+ION'>NI</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.335Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~HMPREF2819_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1739323 Staphylococcus sp. HMSC055H04]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [~~http~~://pdbe.org/5wk0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [https://pdbe.org/5wk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [https://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2C9TMM3_STAAU A0A2C9TMM3_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Staphylococcus sp. ~~hmsc055h04~~]]	+	[[Category: Staphylococcus sp. HMSC055H04]]
-	[[Category: Cook~~, P D~~]]	+	[[Category: Cook PD]]
-	[[Category: Francis~~, J W]]~~	+	[[Category: Francis JW]]
-	~~[[Category: Bacillithiol]]~~	+
-	~~[[Category: Detoxification]]~~	+
-	~~[[Category: Helix bundle]]~~	+
-	~~[[Category: Metalloenzyme]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 09:53, 1 January 2020

OCA — Wed, 01 Jan 2020 09:53:49 GMT

←Older revision		Revision as of 09:53, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of the bacillithiol transferase BstA from Staphylococcus aureus.==		==Crystal structure of the bacillithiol transferase BstA from Staphylococcus aureus.==
-	<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>	+	<StructureSection load='5wk0' size='340' side='right'caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_sp._hmsc055h04 Staphylococcus sp. hmsc055h04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_sp._hmsc055h04 Staphylococcus sp. hmsc055h04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staphylococcus sp. hmsc055h04]]		[[Category: Staphylococcus sp. hmsc055h04]]
	[[Category: Cook, P D]]		[[Category: Cook, P D]]

OCA at 06:51, 4 April 2018

OCA — Wed, 04 Apr 2018 06:51:27 GMT

←Older revision		Revision as of 06:51, 4 April 2018
Line 3:		Line 3:
	<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>		<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_sp._hmsc055h04 Staphylococcus sp. hmsc055h04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF2819_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF2819_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1739323 Staphylococcus sp. HMSC055H04])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [http://pdbe.org/5wk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [http://pdbe.org/5wk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Staphylococcus sp. hmsc055h04]]
	[[Category: Cook, P D]]		[[Category: Cook, P D]]
	[[Category: Francis, J W]]		[[Category: Francis, J W]]

OCA at 06:14, 8 March 2018

OCA — Thu, 08 Mar 2018 06:14:00 GMT

←Older revision		Revision as of 06:14, 8 March 2018
Line 3:		Line 3:
	<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>		<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF2819_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [http://pdbe.org/5wk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [http://pdbe.org/5wk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:18, 22 February 2018

OCA — Thu, 22 Feb 2018 07:18:33 GMT

←Older revision		Revision as of 07:18, 22 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wk0 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the bacillithiol transferase BstA from Staphylococcus aureus.==
		+	<StructureSection load='5wk0' size='340' side='right' caption='[[5wk0]], [[Resolution\|resolution]] 1.33Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wk0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WK0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk0 OCA], [http://pdbe.org/5wk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wk0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacillithiol is a low-molecular weight thiol produced by many gram-positive organisms, including Staphylococcus aureus and Bacillus anthracis. It is the major thiol responsible for maintaining redox homeostasis and cellular detoxification, including inactivation of the antibiotic fosfomycin. The metal-dependent bacillithiol transferase BstA is likely involved in these sorts of detoxification processes, but the exact substrates and enzyme mechanism have not been identified. Here we report the 1.34 A resolution X-ray crystallographic structure of BstA from S. aureus. Our structure confirms that BstA belongs to the YfiT-like metal-dependent hydrolase superfamily. Like YfiT, our structure contains nickel within its active site, but our functional data suggest that BstA utilizes zinc for activity. Although BstA and YfiT both contain a core four helix bundle and coordinate their metal ions in the same fashion, significant differences between the protein structures are described here. Most organisms employ low-molecular weight thiols (LMWTs) to serve as redox buffers and deactivate toxic compounds. Bacillithiol (Scheme S1) is the predominant LMWT in many gram-positive bacteria such as Staphylococcus aureus and Bacillus anthracis.(1-3) It is the preferred thiol cosubstrate for FosB, a bacillithiol-S-transferase responsible for resistance to the antibiotic fosfomycin.(4,5) BstA is another bacillithiol-S-transferase from S. aureus that plays a role in detoxifying electrophilic xenobiotic compounds within the cell by producing bacillithiol-S-conjugated products, which are further processed and removed from the cell.(6) BstA is active against the model electrophilic compound chloro-2,4-dinitrobenzene (CDNB), but the exact electrophilic substrate, or group of substrates, upon which BstA acts has not been identified. BstA belongs to the DinB/YfiT-like superfamily of metal-dependent enzymes.(6,7) Although the structure of the namesake bacillithiol transferase YfiT from B. subtilis has been determined,(8) the study of bacillithiol transferases is still in its infancy, and additional structures are needed to characterize this group of enzymes. Here we present the 1.34 A resolution crystal structure of BstA from S. aureus. The structure clearly demonstrates how the enzyme binds its metal cofactor and gives insight into the types of substrates BstA might bind. Furthermore, we demonstrate that BstA is likely zinc-dependent. This article is protected by copyright. All rights reserved.

-	~~Authors: Cook, P.D~~., Francis, J.W.	+	Structure and Function of the Bacillithiol-S-Transferase BstA from Staphylococcus aureus.,Francis JW, Royer CJ, Cook PD Protein Sci. 2018 Feb 8. doi: 10.1002/pro.3384. PMID:29417696<ref>PMID:29417696</ref>

-	~~Description: Crystal structure~~ of the ~~bacillithiol transferase BstA from Staphylococcus aureus~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Francis, J.W]]	+	<div class="pdbe-citations 5wk0" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Cook, P.D~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cook, P D]]
		+	[[Category: Francis, J W]]
		+	[[Category: Bacillithiol]]
		+	[[Category: Detoxification]]
		+	[[Category: Helix bundle]]
		+	[[Category: Metalloenzyme]]
		+	[[Category: Unknown function]]

OCA: Protected "5wk0" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:54:28 GMT

Protected "5wk0" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:54, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wk0 is ON HOLD until Paper Publication Authors: Cook, P.D., Francis, J.W. Description: Crystal structure of the bacillithiol transferase BstA from...

OCA — Wed, 27 Sep 2017 14:54:27 GMT

New page: '''Unreleased structure''' The entry 5wk0 is ON HOLD until Paper Publication Authors: Cook, P.D., Francis, J.W. Description: Crystal structure of the bacillithiol transferase BstA from...

New page

'''Unreleased structure'''

The entry 5wk0 is ON HOLD until Paper Publication

Authors: Cook, P.D., Francis, J.W.

Description: Crystal structure of the bacillithiol transferase BstA from Staphylococcus aureus.
[[Category: Unreleased Structures]]
[[Category: Francis, J.W]]
[[Category: Cook, P.D]]