5wko - Revision history

OCA at 14:15, 4 October 2023

OCA — Wed, 04 Oct 2023 14:15:50 GMT

←Older revision		Revision as of 14:15, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5wko' size='340' side='right'caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>		<StructureSection load='5wko' size='340' side='right'caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~], [~~http~~://en.wikipedia.org/wiki/~~I09a0 I09a0~~] and [~~http~~://en.wikipedia.org/wiki/~~Influenza_a_virus_~~(a/~~uganda~~/~~muwrp~~-176/2010(~~h1n1~~)) Influenza a virus (a/~~uganda~~/~~muwrp~~-176/2010(~~h1n1~~))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/California/04/2009(H1N1)) Influenza A virus (A/California/04/2009(H1N1))] and [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Uganda/MUWRP-176/2010(H1N1)) Influenza A virus (A/Uganda/MUWRP-176/2010(H1N1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WKO FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>DKFZp686P15220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])~~, ~~IGK@ (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HA (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=641501 I09A0~~]~~), HA ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1487164 Influenza A virus (A/Uganda/MUWRP-176/2010(H1N1))])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.492Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wko OCA], [~~http~~://pdbe.org/5wko PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wko RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wko PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wko ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wko OCA], [https://pdbe.org/5wko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wko RCSB], [https://www.ebi.ac.uk/pdbsum/5wko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/A0A023ZYH9_9INFA A0A023ZYH9_9INFA]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[SAAS:SAAS00842036] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324] [[http://www.uniprot.org/uniprot/C3W5S1_I09A0 C3W5S1_I09A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).[SAAS:SAAS013829_004_327643][RuleBase:RU003324]	+	[https://www.uniprot.org/uniprot/Q6N089_HUMAN Q6N089_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]		*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	*[[3D structures of human antibody\|3D structures of human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: I09a0~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lang, S]]	+	[[Category: Lang S]]
-	[[Category: Wilson, A]]	+	[[Category: Wilson A]]
-	[[Category: Zhu, X]]	+	[[Category: Zhu X]]
-	~~[[Category: 27f3 h1 ha a/california/04/2009]]~~	+
-	~~[[Category: Antiviral protein~~]]	+

OCA at 17:36, 11 December 2019

OCA — Wed, 11 Dec 2019 17:36:27 GMT

←Older revision		Revision as of 17:36, 11 December 2019
Line 1:		Line 1:

	==Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus==		==Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus==
-	<StructureSection load='5wko' size='340' side='right' caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>	+	<StructureSection load='5wko' size='340' side='right'caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human], [http://en.wikipedia.org/wiki/I09a0 I09a0] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/uganda/muwrp-176/2010(h1n1)) Influenza a virus (a/uganda/muwrp-176/2010(h1n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human], [http://en.wikipedia.org/wiki/I09a0 I09a0] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/uganda/muwrp-176/2010(h1n1)) Influenza a virus (a/uganda/muwrp-176/2010(h1n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKO FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5wko" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5wko" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 28:
	[[Category: Human]]		[[Category: Human]]
	[[Category: I09a0]]		[[Category: I09a0]]
		+	[[Category: Large Structures]]
	[[Category: Lang, S]]		[[Category: Lang, S]]
	[[Category: Wilson, A]]		[[Category: Wilson, A]]

OCA at 07:51, 6 December 2017

OCA — Wed, 06 Dec 2017 07:51:24 GMT

←Older revision		Revision as of 07:51, 6 December 2017
Line 3:		Line 3:
	<StructureSection load='5wko' size='340' side='right' caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>		<StructureSection load='5wko' size='340' side='right' caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human], [http://en.wikipedia.org/wiki/I09a0 I09a0] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/uganda/muwrp-176/2010(h1n1)) Influenza a virus (a/uganda/muwrp-176/2010(h1n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wko OCA], [http://pdbe.org/5wko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wko RCSB], [http://www.ebi.ac.uk/pdbsum/5wko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wko ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DKFZp686P15220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), IGK@ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=641501 I09A0]), HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1487164 Influenza A virus (A/Uganda/MUWRP-176/2010(H1N1))])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wko OCA], [http://pdbe.org/5wko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wko RCSB], [http://www.ebi.ac.uk/pdbsum/5wko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: I09a0]]
	[[Category: Lang, S]]		[[Category: Lang, S]]
	[[Category: Wilson, A]]		[[Category: Wilson, A]]

OCA at 07:03, 25 October 2017

OCA — Wed, 25 Oct 2017 07:03:40 GMT

←Older revision		Revision as of 07:03, 25 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wko is ~~ON HOLD~~	+	==Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus==
		+	<StructureSection load='5wko' size='340' side='right' caption='[[5wko]], [[Resolution\|resolution]] 3.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wko]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKO FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wko OCA], [http://pdbe.org/5wko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wko RCSB], [http://www.ebi.ac.uk/pdbsum/5wko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wko ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A023ZYH9_9INFA A0A023ZYH9_9INFA]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[SAAS:SAAS00842036] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324] [[http://www.uniprot.org/uniprot/C3W5S1_I09A0 C3W5S1_I09A0]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).[SAAS:SAAS013829_004_327643][RuleBase:RU003324]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibodies that target both group 1 and group 2 influenza A viruses are valuable for therapeutic and vaccine development, but only a few have been reported to date. Here, we describe a new VH1-69 antibody 27F3 that broadly recognizes heterosubtypic hemagglutinins (HAs) from both group 1 and group 2 influenza A viruses. Structural characterization of 27F3 Fab with A/California/04/2009 (H1N1) hemagglutinin illustrates that 27F3 shares the key binding features observed in other VH1-69 antibodies to the HA stem. Compared to other VH1-69 antibodies, the 27F3 VH domain interacts with the HA stem in a distinct orientation, which alters its epitope and may have influenced its breadth. The diverse rotations of VH1-69 antibodies on the HA stem epitope highlight the different ways that this antibody family can evolve to broadly neutralize influenza A viruses. These results have important implications for understanding how to elicit broad antibody responses against influenza virus.

-	~~Authors: Wilson,~~ A., Lang, S., ~~Zhu~~, X.	+	Antibody 27F3 Broadly Targets Influenza A Group 1 and 2 Hemagglutinins through a Further Variation in VH1-69 Antibody Orientation on the HA Stem.,Lang S, Xie J, Zhu X, Wu NC, Lerner RA, Wilson IA Cell Rep. 2017 Sep 19;20(12):2935-2943. doi: 10.1016/j.celrep.2017.08.084. PMID:28930686<ref>PMID:28930686</ref>

-	~~Description: Crystal structure~~ of ~~antibody 27F3 recognizing~~ the ~~HA from A~~/~~California~~/04/~~2009 (H1N1) influenza virus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5wko" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lang, S]]		[[Category: Lang, S]]
	[[Category: Wilson, A]]		[[Category: Wilson, A]]
	[[Category: Zhu, X]]		[[Category: Zhu, X]]
		+	[[Category: 27f3 h1 ha a/california/04/2009]]
		+	[[Category: Antiviral protein]]

OCA: Protected "5wko" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:55:49 GMT

Protected "5wko" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wko is ON HOLD Authors: Wilson, A., Lang, S., Zhu, X. Description: Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009...

OCA — Wed, 27 Sep 2017 14:55:48 GMT

New page: '''Unreleased structure''' The entry 5wko is ON HOLD Authors: Wilson, A., Lang, S., Zhu, X. Description: Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009...

New page

'''Unreleased structure'''

The entry 5wko is ON HOLD

Authors: Wilson, A., Lang, S., Zhu, X.

Description: Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus
[[Category: Unreleased Structures]]
[[Category: Lang, S]]
[[Category: Wilson, A]]
[[Category: Zhu, X]]