5wkq - Revision history

OCA at 14:15, 4 October 2023

OCA — Wed, 04 Oct 2023 14:15:55 GMT

←Older revision		Revision as of 14:15, 4 October 2023
Line 1:		Line 1:

	==2.10 A resolution structure of IpaB (residues 74-242) from Shigella flexneri==		==2.10 A resolution structure of IpaB (residues 74-242) from Shigella flexneri==
-	<StructureSection load='5wkq' size='340' side='right' caption='[[5wkq]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5wkq' size='340' side='right'caption='[[5wkq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wkq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wkq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WKQ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ipaB~~, ~~CP0128 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkq OCA], [~~http~~://pdbe.org/5wkq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wkq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wkq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkq OCA], [https://pdbe.org/5wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wkq RCSB], [https://www.ebi.ac.uk/pdbsum/5wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IPAB_SHIFL IPAB_SHIFL]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.<ref>PMID:17719540</ref> <ref>PMID:9009343</ref>	+	[https://www.uniprot.org/uniprot/IPAB_SHIFL IPAB_SHIFL] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.<ref>PMID:17719540</ref> <ref>PMID:9009343</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Shigella paradysenteriae weldin 1927~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Barta, M L~~]]	+	[[Category: Shigella flexneri]]
-	[[Category: ~~Battaile, K P~~]]	+	[[Category: Barta ML]]
-	[[Category: ~~Lovell, S~~]]	+	[[Category: Battaile KP]]
-	[[Category: ~~Picking, W D~~]]	+	[[Category: Lovell S]]
-	[[Category: Picking~~, W L~~]]	+	[[Category: Picking WD]]
-	[[Category: ~~Ipab]]~~	+	[[Category: Picking WL]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Shigella]]~~	+
-	~~[[Category: T3ss]]~~	+
-	~~[[Category: Translocon]]~~	+
-	~~[[Category: Type iii secretion~~]]	+

OCA at 08:25, 10 October 2018

OCA — Wed, 10 Oct 2018 08:25:13 GMT

←Older revision		Revision as of 08:25, 10 October 2018
Line 3:		Line 3:
	<StructureSection load='5wkq' size='340' side='right' caption='[[5wkq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5wkq' size='340' side='right' caption='[[5wkq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wkq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wkq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkq OCA], [http://pdbe.org/5wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wkq RCSB], [http://www.ebi.ac.uk/pdbsum/5wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkq ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ipaB, CP0128 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkq OCA], [http://pdbe.org/5wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wkq RCSB], [http://www.ebi.ac.uk/pdbsum/5wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Barta, M L]]		[[Category: Barta, M L]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]

OCA at 05:24, 16 May 2018

OCA — Wed, 16 May 2018 05:24:47 GMT

←Older revision		Revision as of 05:24, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wkq is ~~ON HOLD~~	+	==2.10 A resolution structure of IpaB (residues 74-242) from Shigella flexneri==
		+	<StructureSection load='5wkq' size='340' side='right' caption='[[5wkq]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wkq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WKQ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkq OCA], [http://pdbe.org/5wkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wkq RCSB], [http://www.ebi.ac.uk/pdbsum/5wkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IPAB_SHIFL IPAB_SHIFL]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.<ref>PMID:17719540</ref> <ref>PMID:9009343</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial type III secretion systems (T3SS) are used to inject proteins into mammalian cells to subvert cellular functions. The Shigella T3SS apparatus (T3SA) is comprised of a basal body, cytoplasmic sorting platform and exposed needle with needle "tip complex" (TC). TC maturation occurs when the translocator protein IpaB is recruited to the needle tip where both IpaD and IpaB control secretion induction. IpaB insertion into the host membrane is the first step of translocon pore formation and secretion induction. We employed disruptive insertional mutagenesis, using bacteriophage T4 lysozyme (T4L), within predicted IpaB loops to show how topological features affect TC functions (secretion control, translocon formation and effector secretion). Insertions within the N-terminal half of IpaB were most likely to result in a loss of steady-state secretion control, however, all but the two that were not recognized by the T3SA retained nearly wild-type hemolysis (translocon formation) and invasiveness levels (effector secretion). In contrast, all but one insertion in the C-terminal half of IpaB maintained secretion control but were impaired for hemolysis and invasion. These nature of the data suggest the latter mutants are defective in a post-secretion event, most likely due to impaired interactions with the second translocator protein IpaC. Intriguingly, only two insertion mutants displayed readily detectable T4L on the bacterial surface. The data create a picture in which the makeup and structure of a functional T3SA TC is highly amenable to physical perturbation, indicating that the tertiary structure of IpaB within the TC is more plastic than previously realized.

-	~~Authors~~:	+	Using disruptive insertional mutagenesis to identify the in situ structure-function landscape of the Shigella translocator protein IpaB.,Barta ML, Tachiyama S, Muthuramalingam M, Arizmendi O, Villanueva CE, Ramyar KX, Geisbrecht BV, Lovell S, Battaile KP, Picking WL, Picking WD Protein Sci. 2018 Apr 19. doi: 10.1002/pro.3428. PMID:29672980<ref>PMID:29672980</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5wkq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Barta, M L]]
		+	[[Category: Battaile, K P]]
		+	[[Category: Lovell, S]]
		+	[[Category: Picking, W D]]
		+	[[Category: Picking, W L]]
		+	[[Category: Ipab]]
		+	[[Category: Membrane protein]]
		+	[[Category: Shigella]]
		+	[[Category: T3ss]]
		+	[[Category: Translocon]]
		+	[[Category: Type iii secretion]]

OCA: Protected "5wkq" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 14:55:58 GMT

Protected "5wkq" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wkq is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 14:55:57 GMT

New page: '''Unreleased structure''' The entry 5wkq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wkq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]