5wlh - Revision history

OCA at 14:16, 4 October 2023

OCA — Wed, 04 Oct 2023 14:16:25 GMT

←Older revision		Revision as of 14:16, 4 October 2023
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	<StructureSection load='5wlh' size='340' side='right'caption='[[5wlh]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5wlh' size='340' side='right'caption='[[5wlh]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wlh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WLH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WLH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_NK4A179 Lachnospiraceae bacterium NK4A179]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WLH FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8000103Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5w1h\|5w1h]]~~, ~~[[5w1i\|5w1i]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wlh OCA], [~~http~~://pdbe.org/5wlh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wlh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wlh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wlh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wlh OCA], [https://pdbe.org/5wlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wlh RCSB], [https://www.ebi.ac.uk/pdbsum/5wlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wlh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2D0TCH1_LACNK A0A2D0TCH1_LACNK]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lachnospiraceae bacterium NK4A179]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Doudna~~, J A~~]]	+	[[Category: Doudna JA]]
-	[[Category: Knott~~, G J]]~~	+	[[Category: Knott GJ]]
-	~~[[Category: Nuclease rna binding protein]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna binding protein-rna complex~~]]	+

OCA at 08:53, 30 September 2020

OCA — Wed, 30 Sep 2020 08:53:51 GMT

←Older revision		Revision as of 08:53, 30 September 2020
Line 1:		Line 1:

	==Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)==		==Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)==
-	<StructureSection load='5wlh' size='340' side='right' caption='[[5wlh]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5wlh' size='340' side='right'caption='[[5wlh]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wlh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WLH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WLH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wlh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WLH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WLH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w1h\|5w1h]], [[5w1i\|5w1i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w1h\|5w1h]], [[5w1i\|5w1i]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wlh OCA], [http://pdbe.org/5wlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wlh RCSB], [http://www.ebi.ac.uk/pdbsum/5wlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wlh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wlh OCA], [http://pdbe.org/5wlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wlh RCSB], [http://www.ebi.ac.uk/pdbsum/5wlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wlh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Doudna, J A]]		[[Category: Doudna, J A]]
	[[Category: Knott, G J]]		[[Category: Knott, G J]]

OCA: Protected "5wlh" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 10:10:49 GMT

Protected "5wlh" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:10, 27 September 2017

OCA: New page: ==Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)== == Structural highlights ==
<table><tr><td colspan='2'>[[5wlh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WLH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w1h|5w1h]], [[5w1i|5w1i]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wlh OCA], [http://pdbe.org/5wlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wlh RCSB], [http://www.ebi.ac.uk/pdbsum/5wlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wlh ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A CRISPR-Cas systems include the Cas13a enzyme, an RNA-activated RNase capable of crRNA processing and single-stranded RNA degradation upon target-transcript binding. Here we present the 2.0-A resolution crystal structure of a crRNA-bound Lachnospiraceae bacterium Cas13a (LbaCas13a), representing a recently discovered Cas13a enzyme subtype. This structure and accompanying biochemical experiments define the Cas13a catalytic residues that are directly responsible for crRNA maturation. In addition, the orientation of the foreign-derived target-RNA-specifying sequence in the protein interior explains the conformational gating of Cas13a nuclease activation. These results describe how Cas13a enzymes generate functional crRNAs and how catalytic activity is blocked before target-RNA recognition, with implications for both bacterial immunity and diagnostic applications.

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.,Knott GJ, East-Seletsky A, Cofsky JC, Holton JM, Charles E, O'Connell MR, Doudna JA Nat Struct Mol Biol. 2017 Sep 11. doi: 10.1038/nsmb.3466. PMID:28892041<ref>PMID:28892041</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wlh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Doudna, J A]]
[[Category: Knott, G J]]
[[Category: Nuclease rna binding protein]]
[[Category: Rna binding protein]]
[[Category: Rna binding protein-rna complex]]