5wnk - Revision history

OCA at 14:16, 13 March 2024

OCA — Wed, 13 Mar 2024 14:16:38 GMT

←Older revision		Revision as of 14:16, 13 March 2024
Line 1:		Line 1:

	==Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115==		==Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115==
-	<StructureSection load='5wnk' size='340' side='right' caption='[[5wnk]], [[Resolution\|resolution]] 3.11Å' scene=''>	+	<StructureSection load='5wnk' size='340' side='right'caption='[[5wnk]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WNK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WNK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.11Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wni\|5wni]]~~, [[~~5wnj~~\|~~5wnj]], [[5wnl\|5wnl]], [[5wnm\|5wnm~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Ripk4, Ankrd3, Pkk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [https://pdbe.org/5wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [https://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [~~http~~://pdbe.org/5wnk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE]] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref>	+	[https://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Receptor-interacting protein kinase 4 (RIPK4) is a highly conserved regulator of epidermal differentiation. Members of the RIPK family possess a common kinase domain as well as unique accessory domains that likely dictate subcellular localization and substrate preferences. Mutations in human RIPK4 manifest as Bartsocas-Papas syndrome (BPS), a genetic disorder characterized by severe craniofacial and limb abnormalities. We describe the structure of the murine Ripk4 (MmRipk4) kinase domain, in ATP- and inhibitor-bound forms. The crystallographic dimer of MmRipk4 is similar to those of RIPK2 and BRAF, and we show that the intact dimeric entity is required for MmRipk4 catalytic activity through a series of engineered mutations and cell-based assays. We also assess the impact of BPS mutations on protein structure and activity to elucidate the molecular origins of the disease.	+

-	~~Crystal Structure of Ripk4 Reveals Dimerization~~-~~Dependent Kinase Activity.,Huang CS, Oberbeck N, Hsiao YC, Liu P, Johnson AR, Dixit VM, Hymowitz SG Structure. 2018 May 1;26(5):767~~-~~777.e5. doi: 10.1016/j.str.2018.04.002. Epub 2018, Apr 26. PMID:29706531<ref>PMID:29706531</ref>~~	+	==See Also==
-		+	*[[Ankyrin repeat domain-containing protein 3D structures\|Ankyrin repeat domain-containing protein 3D structures]]
-	~~From MEDLINE®~~/~~PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5wnk" style="background-color:#fffaf0;"><~~/~~div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Non-specific serine/threonine protein kinase~~]]	+	[[Category: Mus musculus]]
-	[[Category: Huang~~, C S~~]]	+	[[Category: Huang CS]]
-	[[Category: Hymowitz~~, S G]]~~	+	[[Category: Hymowitz SG]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transferase-transferase inhibitor complex~~]]	+

OCA at 05:54, 16 May 2018

OCA — Wed, 16 May 2018 05:54:05 GMT

←Older revision		Revision as of 05:54, 16 May 2018
Line 3:		Line 3:
	<StructureSection load='5wnk' size='340' side='right' caption='[[5wnk]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='5wnk' size='340' side='right' caption='[[5wnk]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WNK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WNK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wni\|5wni]], [[5wnj\|5wnj]], [[5wnl\|5wnl]], [[5wnm\|5wnm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wni\|5wni]], [[5wnj\|5wnj]], [[5wnl\|5wnl]], [[5wnm\|5wnm]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ripk4, Ankrd3, Pkk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [http://pdbe.org/5wnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [http://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [http://pdbe.org/5wnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [http://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr>
Line 11:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE]] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref>		[[http://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE]] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Receptor-interacting protein kinase 4 (RIPK4) is a highly conserved regulator of epidermal differentiation. Members of the RIPK family possess a common kinase domain as well as unique accessory domains that likely dictate subcellular localization and substrate preferences. Mutations in human RIPK4 manifest as Bartsocas-Papas syndrome (BPS), a genetic disorder characterized by severe craniofacial and limb abnormalities. We describe the structure of the murine Ripk4 (MmRipk4) kinase domain, in ATP- and inhibitor-bound forms. The crystallographic dimer of MmRipk4 is similar to those of RIPK2 and BRAF, and we show that the intact dimeric entity is required for MmRipk4 catalytic activity through a series of engineered mutations and cell-based assays. We also assess the impact of BPS mutations on protein structure and activity to elucidate the molecular origins of the disease.
		+
		+	Crystal Structure of Ripk4 Reveals Dimerization-Dependent Kinase Activity.,Huang CS, Oberbeck N, Hsiao YC, Liu P, Johnson AR, Dixit VM, Hymowitz SG Structure. 2018 May 1;26(5):767-777.e5. doi: 10.1016/j.str.2018.04.002. Epub 2018, Apr 26. PMID:29706531<ref>PMID:29706531</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wnk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Huang, C S]]		[[Category: Huang, C S]]

OCA at 06:13, 9 May 2018

OCA — Wed, 09 May 2018 06:13:23 GMT

←Older revision		Revision as of 06:13, 9 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wnk is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115==
-		+	<StructureSection load='5wnk' size='340' side='right' caption='[[5wnk]], [[Resolution\|resolution]] 3.11Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WNK FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wni\|5wni]], [[5wnj\|5wnj]], [[5wnl\|5wnl]], [[5wnm\|5wnm]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [http://pdbe.org/5wnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [http://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE]] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Non-specific serine/threonine protein kinase]]
		+	[[Category: Huang, C S]]
		+	[[Category: Hymowitz, S G]]
		+	[[Category: Complex]]
		+	[[Category: Inhibitor]]
		+	[[Category: Kinase]]
		+	[[Category: Transferase]]
		+	[[Category: Transferase-transferase inhibitor complex]]

OCA: Protected "5wnk" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:00:56 GMT

Protected "5wnk" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wnk is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 15:00:55 GMT

New page: '''Unreleased structure''' The entry 5wnk is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wnk is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]