5woy - Revision history

OCA at 10:29, 14 June 2023

OCA — Wed, 14 Jun 2023 10:29:49 GMT

←Older revision		Revision as of 10:29, 14 June 2023
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	==NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra==		==NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra==
-	<StructureSection load='5woy' size='340' side='right'caption='[[5woy~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='5woy' size='340' side='right'caption='[[5woy]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5woy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5woy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WOY FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate--protein_phosphotransferase Phosphoenolpyruvate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.9 2.7.3.9] </span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5woy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woy OCA], [https://pdbe.org/5woy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5woy RCSB], [https://www.ebi.ac.uk/pdbsum/5woy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5woy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5woy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woy OCA], [~~http~~://pdbe.org/5woy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5woy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5woy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5woy ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q8R7R4_CALS4 Q8R7R4_CALS4]] General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).[PIRNR:PIRNR000732]	+	[https://www.uniprot.org/uniprot/Q8R7R4_CALS4 Q8R7R4_CALS4] General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).[PIRNR:PIRNR000732]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphoenolpyruvate--protein phosphotransferase]]~~	+	[[Category: Evangelidis T]]
-	[[Category: Evangelidis, T]]	+	[[Category: Nerli S]]
-	[[Category: Nerli, S]]	+	[[Category: Sgourakis NG]]
-	[[Category: Sgourakis~~, N G~~]]	+	[[Category: Tripsianes K]]
-	[[Category: Tripsianes, K]]	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:13, 7 October 2020

OCA — Wed, 07 Oct 2020 07:13:49 GMT

←Older revision		Revision as of 07:13, 7 October 2020
Line 1:		Line 1:

	==NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra==		==NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra==
-	<StructureSection load='5woy' size='340' side='right' caption='[[5woy]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='5woy' size='340' side='right'caption='[[5woy]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5woy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5woy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WOY FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate--protein_phosphotransferase Phosphoenolpyruvate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.9 2.7.3.9] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate--protein_phosphotransferase Phosphoenolpyruvate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.9 2.7.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5woy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woy OCA], [http://pdbe.org/5woy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5woy RCSB], [http://www.ebi.ac.uk/pdbsum/5woy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5woy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5woy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woy OCA], [http://pdbe.org/5woy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5woy RCSB], [http://www.ebi.ac.uk/pdbsum/5woy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5woy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5woy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5woy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Phosphoenolpyruvate--protein phosphotransferase]]		[[Category: Phosphoenolpyruvate--protein phosphotransferase]]
	[[Category: Evangelidis, T]]		[[Category: Evangelidis, T]]

OCA at 06:14, 7 February 2018

OCA — Wed, 07 Feb 2018 06:14:40 GMT

←Older revision		Revision as of 06:14, 7 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5woy is ~~ON HOLD until Paper~~ Publication	+	==NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra==
		+	<StructureSection load='5woy' size='340' side='right' caption='[[5woy]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5woy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WOY FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate--protein_phosphotransferase Phosphoenolpyruvate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.9 2.7.3.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5woy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woy OCA], [http://pdbe.org/5woy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5woy RCSB], [http://www.ebi.ac.uk/pdbsum/5woy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5woy ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8R7R4_CALS4 Q8R7R4_CALS4]] General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).[PIRNR:PIRNR000732]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Automated methods for NMR structure determination of proteins are continuously becoming more robust. However, current methods addressing larger, more complex targets rely on analyzing 6-10 complementary spectra, suggesting the need for alternative approaches. Here, we describe 4D-CHAINS/autoNOE-Rosetta, a complete pipeline for NOE-driven structure determination of medium- to larger-sized proteins. The 4D-CHAINS algorithm analyzes two 4D spectra recorded using a single, fully protonated protein sample in an iterative ansatz where common NOEs between different spin systems supplement conventional through-bond connectivities to establish assignments of sidechain and backbone resonances at high levels of completeness and with a minimum error rate. The 4D-CHAINS assignments are then used to guide automated assignment of long-range NOEs and structure refinement in autoNOE-Rosetta. Our results on four targets ranging in size from 15.5 to 27.3 kDa illustrate that the structures of proteins can be determined accurately and in an unsupervised manner in a matter of days.

-	~~Authors~~:	+	Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra.,Evangelidis T, Nerli S, Novacek J, Brereton AE, Karplus PA, Dotas RR, Venditti V, Sgourakis NG, Tripsianes K Nat Commun. 2018 Jan 26;9(1):384. doi: 10.1038/s41467-017-02592-z. PMID:29374165<ref>PMID:29374165</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5woy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Phosphoenolpyruvate--protein phosphotransferase]]
		+	[[Category: Evangelidis, T]]
		+	[[Category: Nerli, S]]
		+	[[Category: Sgourakis, N G]]
		+	[[Category: Tripsianes, K]]
		+	[[Category: Transferase]]

OCA: Protected "5woy" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:02:48 GMT

Protected "5woy" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:02, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5woy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 15:02:47 GMT

New page: '''Unreleased structure''' The entry 5woy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5woy is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]