5wq0 - Revision history

OCA at 09:18, 20 March 2024

OCA — Wed, 20 Mar 2024 09:18:19 GMT

←Older revision		Revision as of 09:18, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='5wq0' size='340' side='right'caption='[[5wq0]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5wq0' size='340' side='right'caption='[[5wq0]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wq0]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wq0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenisporosarcina_sp._TG-14 Paenisporosarcina sp. TG-14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WQ0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.604Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq0 OCA], [~~http~~://pdbe.org/5wq0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wq0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wq0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq0 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq0 OCA], [https://pdbe.org/5wq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wq0 RCSB], [https://www.ebi.ac.uk/pdbsum/5wq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq0 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A1W2VN00_9BACL A0A1W2VN00_9BACL]
-	The two-component phosphorelay system is the most prevalent mechanism for sensing and transducing environmental signals in bacteria. Spore formation, which relies on the two-component phosphorelay system, enables the longterm survival of the glacial bacterium Paenisporosarcina sp. TG-14 in the extreme cold environment. Spo0A is a key response regulator of the phosphorelay system in the early stage of spore formation. The protein is composed of a regulatory N-terminal phospho-receiver domain and a DNAbinding C-terminal activator domain. We solved the threedimensional structure of the unphosphorylated (inactive) form of the receiver domain of Spo0A (PaSpo0A-R) from Paenisporosarcina sp. TG-14. A structural comparison with phosphorylated (active form) Spo0A from Bacillus stearothermophilus (BsSpo0A) showed minor notable differences. A molecular dynamics study of a model of the active form and the crystal structures revealed significant differences in the alpha4 helix and the preceding loop region where phosphorylation occurs. Although an oligomerization study of PaSpo0A-R by analytical ultracentrifugation (AUC) has shown that the protein is in a monomeric state in solution, both crosslinking and crystal-packing analyses indicate the possibility of weak dimer formation by a previously undocumented mechanism. Collectively, these observations provide insight into the mechanism of phosphorylation-dependent activation unique to Spo0A.	+
-		+
-	Crystal structure of the inactive state of the receiver domain of Spo0A from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier.,Lee CW, Park SH, Lee SG, Shin SC, Han SJ, Kim HW, Park HH, Kim S, Kim HJ, Park H, Park H, Lee JH J Microbiol. 2017 Mar 9. doi: ~~10.1007~~/~~s12275-017-6599-9. PMID:28281198<ref>PMID:28281198<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5wq0" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lee, C W]]~~	+	[[Category: Paenisporosarcina sp. TG-14]]
-	~~[[Category: Lee, J H]]~~	+	[[Category: Lee CW]]
-	[[Category: Paenisporosarcina sp. tg-14]]	+	[[Category: Lee JH]]
-	[[Category: ~~Signaling protein~~]]	+
-	[[Category: ~~Spo0a~~]]	+

OCA at 07:14, 7 October 2020

OCA — Wed, 07 Oct 2020 07:14:00 GMT

←Older revision		Revision as of 07:14, 7 October 2020
Line 1:		Line 1:

	==Receiver domain of Spo0A from Paenisporosarcina sp. TG-14==		==Receiver domain of Spo0A from Paenisporosarcina sp. TG-14==
-	<StructureSection load='5wq0' size='340' side='right' caption='[[5wq0]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5wq0' size='340' side='right'caption='[[5wq0]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wq0]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wq0]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WQ0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq0 OCA], [http://pdbe.org/5wq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq0 OCA], [http://pdbe.org/5wq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lee, C W]]		[[Category: Lee, C W]]
	[[Category: Lee, J H]]		[[Category: Lee, J H]]

OCA at 08:29, 3 August 2017

OCA — Thu, 03 Aug 2017 08:29:38 GMT

←Older revision		Revision as of 08:29, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wq0 is ~~ON HOLD until Paper~~ Publication	+	==Receiver domain of Spo0A from Paenisporosarcina sp. TG-14==
		+	<StructureSection load='5wq0' size='340' side='right' caption='[[5wq0]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wq0]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WQ0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq0 OCA], [http://pdbe.org/5wq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The two-component phosphorelay system is the most prevalent mechanism for sensing and transducing environmental signals in bacteria. Spore formation, which relies on the two-component phosphorelay system, enables the longterm survival of the glacial bacterium Paenisporosarcina sp. TG-14 in the extreme cold environment. Spo0A is a key response regulator of the phosphorelay system in the early stage of spore formation. The protein is composed of a regulatory N-terminal phospho-receiver domain and a DNAbinding C-terminal activator domain. We solved the threedimensional structure of the unphosphorylated (inactive) form of the receiver domain of Spo0A (PaSpo0A-R) from Paenisporosarcina sp. TG-14. A structural comparison with phosphorylated (active form) Spo0A from Bacillus stearothermophilus (BsSpo0A) showed minor notable differences. A molecular dynamics study of a model of the active form and the crystal structures revealed significant differences in the alpha4 helix and the preceding loop region where phosphorylation occurs. Although an oligomerization study of PaSpo0A-R by analytical ultracentrifugation (AUC) has shown that the protein is in a monomeric state in solution, both crosslinking and crystal-packing analyses indicate the possibility of weak dimer formation by a previously undocumented mechanism. Collectively, these observations provide insight into the mechanism of phosphorylation-dependent activation unique to Spo0A.

-	~~Authors: Lee~~, ~~J.H~~., Lee, C.W.	+	Crystal structure of the inactive state of the receiver domain of Spo0A from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier.,Lee CW, Park SH, Lee SG, Shin SC, Han SJ, Kim HW, Park HH, Kim S, Kim HJ, Park H, Park H, Lee JH J Microbiol. 2017 Mar 9. doi: 10.1007/s12275-017-6599-9. PMID:28281198<ref>PMID:28281198</ref>

-	~~Description: Receiver domain~~ of ~~Spo0A from Paenisporosarcina sp~~. TG-14	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Lee, ~~C.W~~]]	+	<div class="pdbe-citations 5wq0" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Lee, J~~.H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lee, C W]]
		+	[[Category: Lee, J H]]
		+	[[Category: Paenisporosarcina sp. tg-14]]
		+	[[Category: Signaling protein]]
		+	[[Category: Spo0a]]

OCA at 15:44, 4 January 2017

OCA — Wed, 04 Jan 2017 15:44:47 GMT

←Older revision		Revision as of 15:44, 4 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5wq0 is ON HOLD	+	The entry 5wq0 is ON HOLD until Paper Publication

	Authors: Lee, J.H., Lee, C.W.		Authors: Lee, J.H., Lee, C.W.

OCA: Protected "5wq0" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 18:16:20 GMT

Protected "5wq0" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:16, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wq0 is ON HOLD Authors: Lee, J.H., Lee, C.W. Description: Receiver domain of Spo0A from Paenisporosarcina sp. TG-14 [[Category: Unreleased Structu...

OCA — Fri, 09 Dec 2016 18:16:18 GMT

New page: '''Unreleased structure''' The entry 5wq0 is ON HOLD Authors: Lee, J.H., Lee, C.W. Description: Receiver domain of Spo0A from Paenisporosarcina sp. TG-14 [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 5wq0 is ON HOLD

Authors: Lee, J.H., Lee, C.W.

Description: Receiver domain of Spo0A from Paenisporosarcina sp. TG-14
[[Category: Unreleased Structures]]
[[Category: Lee, C.W]]
[[Category: Lee, J.H]]