5wq6 - Revision history

OCA at 16:51, 8 November 2023

OCA — Wed, 08 Nov 2023 16:51:10 GMT

←Older revision		Revision as of 16:51, 8 November 2023
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	<StructureSection load='5wq6' size='340' side='right'caption='[[5wq6]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='5wq6' size='340' side='right'caption='[[5wq6]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wq6]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wq6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WQ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.648Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wpz\|5wpz]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq6 OCA], [~~http~~://pdbe.org/5wq6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wq6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wq6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq6 OCA], [https://pdbe.org/5wq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wq6 RCSB], [https://www.ebi.ac.uk/pdbsum/5wq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jin~~, T C~~]]	+	[[Category: Jin TC]]
-	[[Category: Xiao~~, T S]]~~	+	[[Category: Xiao TS]]
-	~~[[Category: Crystallization tag]]~~	+
-	~~[[Category: Death fold]]~~	+
-	~~[[Category: Mbp]]~~	+
-	~~[[Category: Pyd]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 07:14, 7 October 2020

OCA — Wed, 07 Oct 2020 07:14:19 GMT

←Older revision		Revision as of 07:14, 7 October 2020
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	==Crystal Structure of hMNDA-PYD with MBP tag==		==Crystal Structure of hMNDA-PYD with MBP tag==
-	<StructureSection load='5wq6' size='340' side='right' caption='[[5wq6]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='5wq6' size='340' side='right'caption='[[5wq6]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wq6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wq6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WQ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=~~MAL~~:~~MALTOSE~~'>~~MAL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wpz\|5wpz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wpz\|5wpz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq6 OCA], [http://pdbe.org/5wq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq6 OCA], [http://pdbe.org/5wq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Jin, T C]]		[[Category: Jin, T C]]
	[[Category: Xiao, T S]]		[[Category: Xiao, T S]]

OCA at 17:47, 10 March 2017

OCA — Fri, 10 Mar 2017 17:47:59 GMT

←Older revision		Revision as of 17:47, 10 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5wq6 is ~~ON HOLD~~	+	==Crystal Structure of hMNDA-PYD with MBP tag==
		+	<StructureSection load='5wq6' size='340' side='right' caption='[[5wq6]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wq6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WQ6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wpz\|5wpz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq6 OCA], [http://pdbe.org/5wq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallization chaperones have been used to facilitate the crystallization of challenging proteins. Even though the maltose-binding protein (MBP) is one of the most commonly used crystallization chaperones, the design of optimal expression constructs for crystallization of MBP fusion proteins remains a challenge. To increase the success rate of MBP-facilitated crystallization, a series of expression vectors have been designed with either a short flexible linker or a set of rigid helical linkers. Seven death domain superfamily members were tested for crystallization with this set of vectors, six of which had never been crystallized before. All of the seven targets were crystallized, and their structures were determined using at least one of the vectors. Our successful crystallization of all of the targets demonstrates the validity of our approach and expands the arsenal of the crystallization chaperone toolkit, which may be applicable to crystallization of other difficult protein targets, as well as to other crystallization chaperones.

-	~~Authors: Jin~~, T~~.C.~~, ~~Xiao~~, T.S.	+	Design of an expression system to enhance MBP-mediated crystallization.,Jin T, Chuenchor W, Jiang J, Cheng J, Li Y, Fang K, Huang M, Smith P, Xiao TS Sci Rep. 2017 Jan 23;7:40991. doi: 10.1038/srep40991. PMID:28112203<ref>PMID:28112203</ref>

-	~~Description: Crystal Structure~~ of ~~hMNDA-PYD with MBP tag~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Jin, T.C]]	+	<div class="pdbe-citations 5wq6" style="background-color:#fffaf0;"></div>
-	[[Category: Xiao, T.S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jin, T C]]
		+	[[Category: Xiao, T S]]
		+	[[Category: Crystallization tag]]
		+	[[Category: Death fold]]
		+	[[Category: Mbp]]
		+	[[Category: Pyd]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "5wq6" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 18:16:47 GMT

Protected "5wq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:16, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wq6 is ON HOLD Authors: Jin, T.C., Xiao, T.S. Description: Crystal Structure of hMNDA-PYD with MBP tag Category: Unreleased Structures [[Categ...

OCA — Fri, 09 Dec 2016 18:16:46 GMT

New page: '''Unreleased structure''' The entry 5wq6 is ON HOLD Authors: Jin, T.C., Xiao, T.S. Description: Crystal Structure of hMNDA-PYD with MBP tag Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 5wq6 is ON HOLD

Authors: Jin, T.C., Xiao, T.S.

Description: Crystal Structure of hMNDA-PYD with MBP tag
[[Category: Unreleased Structures]]
[[Category: Jin, T.C]]
[[Category: Xiao, T.S]]