5wsu - Revision history

OCA at 16:53, 8 November 2023

OCA — Wed, 08 Nov 2023 16:53:10 GMT

←Older revision		Revision as of 16:53, 8 November 2023
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	==Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM==		==Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM==
-	<StructureSection load='5wsu' size='340' side='right' caption='[[5wsu]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5wsu' size='340' side='right'caption='[[5wsu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wsu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WSU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WSU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wsu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WSU FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5wst\|5wst]], [[5wsv\|5wsv]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN]), Myo7a, Myo7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wsu OCA], [https://pdbe.org/5wsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wsu RCSB], [https://www.ebi.ac.uk/pdbsum/5wsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wsu ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wsu OCA], [~~http~~://pdbe.org/5wsu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wsu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wsu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wsu ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/~~MYO7A_MOUSE MYO7A_MOUSE~~]~~] Defects~~ in ~~Myo7a~~ are the cause of the ~~shaker-1 (sh-1) phenotype which affects only the inner ear~~. Sh-1 homozygote mutants show hyperactivity, head tossing and circling due to vestibular dysfunction, together with typical neuroepithelial-type cochlear defects involving dysfunction and progressive degeneration of the ~~organ~~ of ~~Corti~~.~~<ref>PMID:7870172</ref>~~	+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~MYO7A_MOUSE MYO7A_MOUSE~~]] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the ~~retina, plays an important role in the renewal~~ of ~~the outer photoreceptor disks. Plays an important role in the distribution and migration~~ of ~~retinal pigment epithelial (RPE) melanosomes and phagosomes~~, and ~~in the regulation of opsin transport in retinal photoreceptors~~. ~~Mediates intracellular transport of RPE65 in~~ the ~~retina pigment epithelium. In~~ the ~~inner ear, plays an important role in differentiation, morphogenesis~~ and ~~organization of cochlear hair cell bundles~~. ~~Motor protein that~~ is a ~~part of~~ the ~~functional network formed by USH1C, USH1G, CDH23~~ and ~~MYO7A that mediates mechanotransduction in cochlear hair cells~~. ~~Required for normal hearing~~. ~~Involved in hair~~-~~cell vesicle trafficking~~ of ~~aminoglycosides, which are known to induce ototoxicity~~.<ref>PMID:~~21493626~~</ref> <ref>PMID:~~21709241~~</ref>	+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5wsu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5wsu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
		+	*[[Myosin 3D Structures\|Myosin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: Chen, Y]]	+	[[Category: Mus musculus]]
-	[[Category: Deng, Y]]	+	[[Category: Chen Y]]
-	[[Category: Li, J]]	+	[[Category: Deng Y]]
-	[[Category: Lu, Q]]	+	[[Category: Li J]]
-	[[Category: Zhang, M]]	+	[[Category: Lu Q]]
-	~~[[Category: Calcium signaling]]~~	+	[[Category: Zhang M]]
-	~~[[Category: Calmodulin]]~~	+
-	~~[[Category: Molecular motor]]~~	+
-	~~[[Category: Motor protein-calcium binding protein complex]]~~	+
-	~~[[Category: Protein complex~~]]	+

OCA at 11:02, 16 November 2017

OCA — Thu, 16 Nov 2017 11:02:25 GMT

←Older revision		Revision as of 11:02, 16 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wsu is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM==
		+	<StructureSection load='5wsu' size='340' side='right' caption='[[5wsu]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wsu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WSU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WSU FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wst\|5wst]], [[5wsv\|5wsv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Myo7a, Myo7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wsu OCA], [http://pdbe.org/5wsu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wsu RCSB], [http://www.ebi.ac.uk/pdbsum/5wsu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wsu ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/MYO7A_MOUSE MYO7A_MOUSE]] Defects in Myo7a are the cause of the shaker-1 (sh-1) phenotype which affects only the inner ear. Sh-1 homozygote mutants show hyperactivity, head tossing and circling due to vestibular dysfunction, together with typical neuroepithelial-type cochlear defects involving dysfunction and progressive degeneration of the organ of Corti.<ref>PMID:7870172</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYO7A_MOUSE MYO7A_MOUSE]] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.<ref>PMID:21493626</ref> <ref>PMID:21709241</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Several unconventional myosins contain a highly charged single alpha helix (SAH) immediately following the calmodulin (CaM) binding IQ motifs, functioning to extend lever arms of these myosins. How such SAH is connected to the IQ motifs and whether the conformation of the IQ motifs-SAH segments are regulated by Ca2+ fluctuations are not known. Here, we demonstrate by solving its crystal structure that the predicted SAH of myosin VIIa (Myo7a) forms a stable SAH. The structure of Myo7a IQ5-SAH segment in complex with apo-CaM reveals that the SAH sequence can extend the length of the Myo7a lever arm. Although Ca2+-CaM remains bound to IQ5-SAH, the Ca2+-induced CaM binding mode change softens the conformation of the IQ5-SAH junction, revealing a Ca2+-induced lever arm flexibility change for Myo7a. We further demonstrate that the last IQ motif of several other myosins also binds to both apo- and Ca2+-CaM, suggesting a common Ca2+-induced conformational regulation mechanism.

-	~~Authors~~:	+	Ca2+-Induced Rigidity Change of the Myosin VIIa IQ Motif-Single alpha Helix Lever Arm Extension.,Li J, Chen Y, Deng Y, Unarta IC, Lu Q, Huang X, Zhang M Structure. 2017 Apr 4;25(4):579-591.e4. doi: 10.1016/j.str.2017.02.002. Epub 2017, Mar 2. PMID:28262393<ref>PMID:28262393</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5wsu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Lk3 transgenic mice]]
		+	[[Category: Chen, Y]]
		+	[[Category: Deng, Y]]
		+	[[Category: Li, J]]
		+	[[Category: Lu, Q]]
		+	[[Category: Zhang, M]]
		+	[[Category: Calcium signaling]]
		+	[[Category: Calmodulin]]
		+	[[Category: Molecular motor]]
		+	[[Category: Motor protein-calcium binding protein complex]]
		+	[[Category: Protein complex]]

OCA: Protected "5wsu" [edit=sysop:move=sysop]

OCA — Thu, 22 Dec 2016 20:40:15 GMT

Protected "5wsu" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:40, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wsu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 22 Dec 2016 20:40:13 GMT

New page: '''Unreleased structure''' The entry 5wsu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wsu is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]