5wta - Revision history

OCA at 16:53, 8 November 2023

OCA — Wed, 08 Nov 2023 16:53:42 GMT

←Older revision		Revision as of 16:53, 8 November 2023
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	<StructureSection load='5wta' size='340' side='right'caption='[[5wta]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5wta' size='340' side='right'caption='[[5wta]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wta]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WTA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wta]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTA FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~5wtb~~\|~~5wtb~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wta OCA], [~~http~~://pdbe.org/5wta PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wta RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wta PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wta ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wta OCA], [https://pdbe.org/5wta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wta RCSB], [https://www.ebi.ac.uk/pdbsum/5wta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wta ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SDRE_STAAM SDRE_STAAM]] Cell surface-associated protein causing human platelet aggregation. Appears to be involved in the aggregation of host platelets thus playing a role in infective endocarditis. May bind calcium (By similarity).	+	[https://www.uniprot.org/uniprot/SDRE_STAAM SDRE_STAAM] Cell surface-associated protein causing human platelet aggregation. Appears to be involved in the aggregation of host platelets thus playing a role in infective endocarditis. May bind calcium (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hang, T]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: Wang, C]]	+	[[Category: Hang T]]
-	[[Category: Wu, M]]	+	[[Category: Wang C]]
-	[[Category: Yang, Y]]	+	[[Category: Wu M]]
-	[[Category: Zang, J]]	+	[[Category: Yang Y]]
-	[[Category: Zhang, M]]	+	[[Category: Zang J]]
-	[[Category: Zhang, X]]	+	[[Category: Zhang M]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang X]]
-	~~[[Category: Cell adhesion]]~~	+	[[Category: Zhang Y]]
-	~~[[Category: Complement evasion]]~~	+
-	~~[[Category: Sdr family]]~~	+
-	~~[[Category: Staphylococcus aureus]]~~	+
-	~~[[Category: Surface protein~~]]	+

OCA at 07:16, 7 October 2020

OCA — Wed, 07 Oct 2020 07:16:23 GMT

←Older revision		Revision as of 07:16, 7 October 2020
Line 1:		Line 1:

	==Crystal Structure of Staphylococcus aureus SdrE apo form==		==Crystal Structure of Staphylococcus aureus SdrE apo form==
-	<StructureSection load='5wta' size='340' side='right' caption='[[5wta]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5wta' size='340' side='right'caption='[[5wta]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wta]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WTA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wta]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WTA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wtb\|5wtb]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wtb\|5wtb]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wta OCA], [http://pdbe.org/5wta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wta RCSB], [http://www.ebi.ac.uk/pdbsum/5wta PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wta ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wta OCA], [http://pdbe.org/5wta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wta RCSB], [http://www.ebi.ac.uk/pdbsum/5wta PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wta ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hang, T]]		[[Category: Hang, T]]
	[[Category: Wang, C]]		[[Category: Wang, C]]

OCA at 16:23, 20 October 2017

OCA — Fri, 20 Oct 2017 16:23:05 GMT

←Older revision		Revision as of 16:23, 20 October 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5wta is ~~ON HOLD~~	+	==Crystal Structure of Staphylococcus aureus SdrE apo form==
		+	<StructureSection load='5wta' size='340' side='right' caption='[[5wta]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wta]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTA FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wtb\|5wtb]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wta OCA], [http://pdbe.org/5wta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wta RCSB], [http://www.ebi.ac.uk/pdbsum/5wta PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wta ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SDRE_STAAM SDRE_STAAM]] Cell surface-associated protein causing human platelet aggregation. Appears to be involved in the aggregation of host platelets thus playing a role in infective endocarditis. May bind calcium (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Complement factor H (CFH) is a soluble complement regulatory protein essential for the down-regulation of the alternative pathway on interaction with specific markers on the host cell surface. It recognizes the complement component 3b (C3b) and 3d (C3d) fragments in addition to self cell markers (i.e. glycosaminoglycans, sialic acid) to distinguish host cells that deserve protection from pathogens that should be eliminated. The Staphylococcus aureus surface protein serine-aspartate repeat protein E (SdrE) was previously reported to bind human CFH as an immune-evasion tactic. However, the molecular mechanism underlying SdrE-CFH-mediated immune evasion remains unknown. In the present study, we identified a novel region at CFH's C-terminus (CFH1206-1226), which binds SdrE N2 and N3 domains (SdrEN2N3) with high affinity, and determined the crystal structures of apo-SdrEN2N3 and the SdrEN2N3-CFH1206-1226 complex. Comparison of the structure of the CFH-SdrE complex with other CFH structures reveals that CFH's C-terminal tail flips from the main body to insert into the ligand-binding groove of SdrE. In addition, SdrEN2N3 adopts a 'close' state in the absence of CFH, which undergoes a large conformational change on CFH binding, suggesting a novel 'close, dock, lock and latch' (CDLL) mechanism for SdrE to recognize its ligand. Our findings imply that SdrE functions as a 'clamp' to capture CFH's C-terminal tail via a unique CDLL mechanism and sequesters CFH on the surface of S. aureus for complement evasion.

-	~~Authors: Wu~~, M., Zhang, Y., Hang, T., Wang, C., Yang, Y., Zang, J., Zhang, M., Zhang, X.	+	Staphylococcus aureus SdrE captures complement factor H's C-terminus via a novel 'close, dock, lock and latch' mechanism for complement evasion.,Zhang Y, Wu M, Hang T, Wang C, Yang Y, Pan W, Zang J, Zhang M, Zhang X Biochem J. 2017 May 4;474(10):1619-1631. doi: 10.1042/BCJ20170085. PMID:28258151<ref>PMID:28258151</ref>

-	~~Description: Crystal Structure~~ of ~~Staphylococcus aureus SdrE apo form~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5wta" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Hang, T]]		[[Category: Hang, T]]
-	[[Category: ~~Zang~~, J]]	+	[[Category: Wang, C]]
		+	[[Category: Wu, M]]
	[[Category: Yang, Y]]		[[Category: Yang, Y]]
		+	[[Category: Zang, J]]
		+	[[Category: Zhang, M]]
	[[Category: Zhang, X]]		[[Category: Zhang, X]]
	[[Category: Zhang, Y]]		[[Category: Zhang, Y]]
-	[[Category: ~~Zhang, M~~]]	+	[[Category: Cell adhesion]]
-	[[Category: ~~Wu, M~~]]	+	[[Category: Complement evasion]]
-	[[Category: ~~Wang, C~~]]	+	[[Category: Sdr family]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Surface protein]]

OCA at 19:50, 15 March 2017

OCA — Wed, 15 Mar 2017 19:50:51 GMT

←Older revision		Revision as of 19:50, 15 March 2017
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hang, T]]		[[Category: Hang, T]]
		+	[[Category: Zang, J]]
	[[Category: Yang, Y]]		[[Category: Yang, Y]]
	[[Category: Zhang, X]]		[[Category: Zhang, X]]
	[[Category: Zhang, Y]]		[[Category: Zhang, Y]]
-	[[Category: Wu, M]]
-	[[Category: Zang, J]]
	[[Category: Zhang, M]]		[[Category: Zhang, M]]
		+	[[Category: Wu, M]]
	[[Category: Wang, C]]		[[Category: Wang, C]]

OCA: Protected "5wta" [edit=sysop:move=sysop]

OCA — Thu, 22 Dec 2016 20:41:08 GMT

Protected "5wta" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:41, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wta is ON HOLD Authors: Wu, M., Zhang, Y., Hang, T., Wang, C., Yang, Y., Zang, J., Zhang, M., Zhang, X. Description: Crystal Structure of Staphylo...

OCA — Thu, 22 Dec 2016 20:41:07 GMT

New page: '''Unreleased structure''' The entry 5wta is ON HOLD Authors: Wu, M., Zhang, Y., Hang, T., Wang, C., Yang, Y., Zang, J., Zhang, M., Zhang, X. Description: Crystal Structure of Staphylo...

New page

'''Unreleased structure'''

The entry 5wta is ON HOLD

Authors: Wu, M., Zhang, Y., Hang, T., Wang, C., Yang, Y., Zang, J., Zhang, M., Zhang, X.

Description: Crystal Structure of Staphylococcus aureus SdrE apo form
[[Category: Unreleased Structures]]
[[Category: Hang, T]]
[[Category: Yang, Y]]
[[Category: Zhang, X]]
[[Category: Zhang, Y]]
[[Category: Wu, M]]
[[Category: Zang, J]]
[[Category: Zhang, M]]
[[Category: Wang, C]]