5www - Revision history

OCA at 07:50, 22 November 2023

OCA — Wed, 22 Nov 2023 07:50:27 GMT

←Older revision		Revision as of 07:50, 22 November 2023
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	<StructureSection load='5www' size='340' side='right'caption='[[5www]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5www' size='340' side='right'caption='[[5www]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5www]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5www]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WWW FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5wwx\|5wwx]], [[5wwz\|5wwz]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.798Å</td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RING~~-~~type_E3_ubiquitin_transferase RING-type E3 ubiquitin transferase]~~, ~~with EC number~~ [~~http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.27 2.3.2.27] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5www FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5www OCA], [https://pdbe.org/5www PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5www RCSB], [https://www.ebi.ac.uk/pdbsum/5www PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5www ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5www FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5www OCA], [~~http~~://pdbe.org/5www PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5www RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5www PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5www ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN]] Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.<ref>PMID:17015768</ref>	+	[https://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN] Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.<ref>PMID:17015768</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN]] E3 ubiquitin ligase responsible for the post-transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation.<ref>PMID:22863774</ref> <ref>PMID:23446422</ref>	+	[https://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN] E3 ubiquitin ligase responsible for the post-transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation.<ref>PMID:22863774</ref> <ref>PMID:23446422</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RING-type E3 ubiquitin transferase~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Gong, Q]]	+	[[Category: Gong Q]]
-	[[Category: Li, F]]	+	[[Category: Li F]]
-	[[Category: Wang, C]]	+	[[Category: Wang C]]
-	[[Category: Yang, L]]	+	[[Category: Yang L]]
-	~~[[Category: Kh1]]~~	+
-	~~[[Category: Mex-3c]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rna binding protein-rna complex~~]]	+

OCA at 07:18, 7 October 2020

OCA — Wed, 07 Oct 2020 07:18:51 GMT

←Older revision		Revision as of 07:18, 7 October 2020
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	==Crystal structure of the KH1 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA==		==Crystal structure of the KH1 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA==
-	<StructureSection load='5www' size='340' side='right' caption='[[5www]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5www' size='340' side='right'caption='[[5www]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5www]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5www]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WWW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wwx\|5wwx]], [[5wwz\|5wwz]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wwx\|5wwx]], [[5wwz\|5wwz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RING-type_E3_ubiquitin_transferase RING-type E3 ubiquitin transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.27 2.3.2.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RING-type_E3_ubiquitin_transferase RING-type E3 ubiquitin transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.27 2.3.2.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5www FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5www OCA], [http://pdbe.org/5www PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5www RCSB], [http://www.ebi.ac.uk/pdbsum/5www PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5www ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5www FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5www OCA], [http://pdbe.org/5www PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5www RCSB], [http://www.ebi.ac.uk/pdbsum/5www PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5www ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: RING-type E3 ubiquitin transferase]]		[[Category: RING-type E3 ubiquitin transferase]]
	[[Category: Gong, Q]]		[[Category: Gong, Q]]

OCA at 10:59, 24 August 2017

OCA — Thu, 24 Aug 2017 10:59:38 GMT

←Older revision		Revision as of 10:59, 24 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5www is ~~ON HOLD~~ ~~until Jan 05 2019~~	+	==Crystal structure of the KH1 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA==
		+	<StructureSection load='5www' size='340' side='right' caption='[[5www]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5www]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWW FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wwx\|5wwx]], [[5wwz\|5wwz]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RING-type_E3_ubiquitin_transferase RING-type E3 ubiquitin transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.27 2.3.2.27] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5www FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5www OCA], [http://pdbe.org/5www PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5www RCSB], [http://www.ebi.ac.uk/pdbsum/5www PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5www ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN]] Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.<ref>PMID:17015768</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MEX3C_HUMAN MEX3C_HUMAN]] E3 ubiquitin ligase responsible for the post-transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation.<ref>PMID:22863774</ref> <ref>PMID:23446422</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MEX-3 is a KH domain-containing RNA-binding protein, first identified as a translational repressor in Caenorhabditis elegans, while its four orthologs (MEX-3A-D) in human and mouse were subsequently found to have E3 ubiquitin ligase activity mediated by a RING domain and critical for RNA degradation. Current evidence implicates human MEX-3C in many essential biological processes and suggests a strong connection with immune diseases and carcinogenesis. The highly conserved dual KH domains in MEX-3 proteins enable RNA binding and are essential for the recognition of the 3' UTR and posttranscriptional regulation of MEX-3 target transcripts. However, the molecular mechanisms of translational repression and the consensus RNA sequence recognized by the MEX-3C KH domain are unknown. Here, using X-ray crystallography and isothermal titration calorimetry, we investigated the RNA-binding activity and selectivity of human MEX-3C dual KH domains. Our high-resolution crystal structures of individual KH domains complexed with a noncanonical U-rich and a GA-rich RNA sequences revealed that the KH1/2 domains of hMEX-3C bound MRE10, a 10-mer RNA (5'-CAGAGUUUAG-3') consisting of an eight-nucleotide MEX-3-recognition element (MRE) motif, with high affinity. Of note, we also identified a consensus RNA motif recognized by human MEX-3C. The potential RNA-binding sites in the 3' UTR of the human leukocyte antigen serotype (HLA-A2) mRNA were mapped with this RNA-binding motif and were further confirmed by fluorescence polarization. The binding motif identified here will provide valuable information for future investigations of the functional pathways controlled by human MEX-3C and for predicting potential mRNAs regulated by this enzyme.

-	~~Authors~~:	+	The human RNA-binding protein and E3 ligase MEX-3C binds the MEX-3-recognition element (MRE) motif with high affinity.,Yang L, Wang C, Li F, Zhang J, Nayab A, Wu J, Shi Y, Gong Q J Biol Chem. 2017 Aug 14. pii: jbc.M117.797746. doi: 10.1074/jbc.M117.797746. PMID:28808060<ref>PMID:28808060</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5www" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RING-type E3 ubiquitin transferase]]
		+	[[Category: Gong, Q]]
		+	[[Category: Li, F]]
		+	[[Category: Wang, C]]
		+	[[Category: Yang, L]]
		+	[[Category: Kh1]]
		+	[[Category: Mex-3c]]
		+	[[Category: Rna]]
		+	[[Category: Rna binding protein-rna complex]]

OCA at 22:32, 25 January 2017

OCA — Wed, 25 Jan 2017 22:32:11 GMT

←Older revision		Revision as of 22:32, 25 January 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5www is ON HOLD	+	The entry 5www is ON HOLD until Jan 05 2019

	Authors:		Authors:

OCA: Protected "5www" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 15:17:56 GMT

Protected "5www" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:17, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5www is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Jan 2017 15:17:54 GMT

New page: '''Unreleased structure''' The entry 5www is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5www is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]