5wym - Revision history

OCA at 07:51, 22 November 2023

OCA — Wed, 22 Nov 2023 07:51:41 GMT

←Older revision		Revision as of 07:51, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5wym' size='340' side='right'caption='[[5wym]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5wym' size='340' side='right'caption='[[5wym]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wym]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WYM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wym]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WYM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wym OCA], [~~http~~://pdbe.org/5wym PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wym RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wym PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wym ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wym OCA], [https://pdbe.org/5wym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wym RCSB], [https://www.ebi.ac.uk/pdbsum/5wym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wym ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	*[[3D structures of human antibody\|3D structures of human antibody]]		*[[3D structures of human antibody\|3D structures of human antibody]]
	== References ==		== References ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Li, S~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Xu, L~~]]	+	[[Category: Li S]]
-	[[Category: ~~Antibody]]~~	+	[[Category: Xu L]]
-	~~[[Category: Connexin26]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Scfv~~]]	+

OCA at 07:20, 7 October 2020

OCA — Wed, 07 Oct 2020 07:20:13 GMT

←Older revision		Revision as of 07:20, 7 October 2020
Line 1:		Line 1:

	==Crystal structure of an anti-connexin26 scFv==		==Crystal structure of an anti-connexin26 scFv==
-	<StructureSection load='5wym' size='340' side='right' caption='[[5wym]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5wym' size='340' side='right'caption='[[5wym]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wym]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYM OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WYM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wym]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WYM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wym OCA], [http://pdbe.org/5wym PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wym RCSB], [http://www.ebi.ac.uk/pdbsum/5wym PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wym ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wym OCA], [http://pdbe.org/5wym PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wym RCSB], [http://www.ebi.ac.uk/pdbsum/5wym PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wym ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 5wym" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5wym" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of human antibody\|3D structures of human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Li, S]]		[[Category: Li, S]]
	[[Category: Xu, L]]		[[Category: Xu, L]]

OCA at 18:24, 24 January 2018

OCA — Wed, 24 Jan 2018 18:24:55 GMT

←Older revision		Revision as of 18:24, 24 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wym is ~~ON HOLD~~	+	==Crystal structure of an anti-connexin26 scFv==
		+	<StructureSection load='5wym' size='340' side='right' caption='[[5wym]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wym]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WYM FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wym OCA], [http://pdbe.org/5wym PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wym RCSB], [http://www.ebi.ac.uk/pdbsum/5wym PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wym ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Background: Mutations leading to changes in properties, regulation, or expression of connexin-made channels have been implicated in 28 distinct human hereditary diseases. Eight of these result from variants of connexin 26 (Cx26), a protein critically involved in cell-cell signaling in the inner ear and skin. Lack of non-toxic drugs with defined mechanisms of action poses a serious obstacle to therapeutic interventions for diseases caused by mutant connexins. In particular, molecules that specifically modulate connexin hemichannel function without affecting gap junction channels are considered of primary importance for the study of connexin hemichannel role in physiological as well as pathological conditions. Monoclonal antibodies developed in the last three decades have become the most important class of therapeutic biologicals. Recombinant methods permit rapid selection and improvement of monoclonal antibodies from libraries with large diversity. Methods: By screening a combinatorial library of human single-chain fragment variable (scFv) antibodies expressed in phage, we identified a candidate that binds an extracellular epitope of Cx26. We characterized antibody action using a variety of biochemical and biophysical assays in HeLa cells, organotypic cultures of mouse cochlea and human keratinocyte-derived cells. Results: We determined that the antibody is a remarkably efficient, non-toxic, and completely reversible inhibitor of hemichannels formed by connexin 26 and does not affect direct cell-cell communication via gap junction channels. Importantly, we also demonstrate that the antibody efficiently inhibits hyperative mutant Cx26 hemichannels implicated in autosomal dominant non-syndromic hearing impairment accompanied by keratitis and hystrix-like ichthyosis-deafness (KID/HID) syndrome. We solved the crystal structure of the antibody, identified residues that are critical for binding and used molecular dynamics to uncover its mechanism of action. Conclusions: Although further studies will be necessary to validate the effect of the antibody in vivo, the methodology described here can be extended to select antibodies against hemichannels composed by other connexin isoforms and, consequently, to target other pathologies associated with hyperactive hemichannels. Our study highlights the potential of this approach and identifies connexins as therapeutic targets addressable by screening phage display libraries expressing human randomized antibodies.

-	~~Authors:~~ Li, S., Xu, L.	+	Design and Characterization of a Human Monoclonal Antibody that Modulates Mutant Connexin 26 Hemichannels Implicated in Deafness and Skin Disorders.,Xu L, Carrer A, Zonta F, Qu Z, Ma P, Li S, Ceriani F, Buratto D, Crispino G, Zorzi V, Ziraldo G, Bruno F, Nardin C, Peres C, Mazzarda F, Salvatore AM, Raspa M, Scavizzi F, Chu Y, Xie S, Yang X, Liao J, Liu X, Wang W, Wang S, Yang G, Lerner RA, Mammano F Front Mol Neurosci. 2017 Sep 22;10:298. doi: 10.3389/fnmol.2017.00298., eCollection 2017. PMID:29018324<ref>PMID:29018324</ref>

-	~~Description: Crystal structure~~ of ~~an anti-connexin26 scFv~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5wym" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Li, S]]		[[Category: Li, S]]
	[[Category: Xu, L]]		[[Category: Xu, L]]
		+	[[Category: Antibody]]
		+	[[Category: Connexin26]]
		+	[[Category: Immune system]]
		+	[[Category: Scfv]]

OCA at 12:06, 5 April 2017

OCA — Wed, 05 Apr 2017 12:06:40 GMT

←Older revision		Revision as of 12:06, 5 April 2017
Line 3:		Line 3:
	The entry 5wym is ON HOLD		The entry 5wym is ON HOLD

-	Authors: ~~Sheng~~ Li, ~~Liang~~ Xu	+	Authors: Li, S., Xu, L.

-	Description: ~~crystal~~ structure of an anti-connexin26 scFv	+	Description: Crystal structure of an anti-connexin26 scFv
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Sheng~~ Li, ~~Liang~~ Xu]]	+	[[Category: Li, S]]
		+	[[Category: Xu, L]]

OCA: Protected "5wym" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 15:21:56 GMT

Protected "5wym" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:21, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5wym is ON HOLD Authors: Sheng Li, Liang Xu Description: crystal structure of an anti-connexin26 scFv Category: Unreleased Structures [[Categor...

OCA — Wed, 18 Jan 2017 15:21:54 GMT

New page: '''Unreleased structure''' The entry 5wym is ON HOLD Authors: Sheng Li, Liang Xu Description: crystal structure of an anti-connexin26 scFv Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 5wym is ON HOLD

Authors: Sheng Li, Liang Xu

Description: crystal structure of an anti-connexin26 scFv
[[Category: Unreleased Structures]]
[[Category: Sheng Li, Liang Xu]]