5x0z - Revision history

OCA at 07:52, 22 November 2023

OCA — Wed, 22 Nov 2023 07:52:42 GMT

←Older revision		Revision as of 07:52, 22 November 2023
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	==Crystal structure of FliM-SpeE complex from H. pylori==		==Crystal structure of FliM-SpeE complex from H. pylori==
-	<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5x0z' size='340' side='right'caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x0z]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Campylobacter_pylori Campylobacter~~ pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x0z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X0Z FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FLC:CITRATE+ANION'>FLC</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~speE, HP_0832 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=85962 Campylobacter pylori]), HP_1031 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0z OCA], [~~http~~://pdbe.org/5x0z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x0z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x0z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0z OCA], [https://pdbe.org/5x0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x0z RCSB], [https://www.ebi.ac.uk/pdbsum/5x0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SPEE_HELPY SPEE_HELPY]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor.<ref>PMID:16009566</ref>	+	[https://www.uniprot.org/uniprot/SPEE_HELPY SPEE_HELPY] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor.<ref>PMID:16009566</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5x0z" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5x0z" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Campylobacter~~ pylori]]	+	[[Category: Helicobacter pylori 26695]]
-	[[Category: ~~Au, S W.N~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Zhang, H]]~~	+	[[Category: Au SWN]]
-	~~[[Category: Flagellar motor~~]]	+	[[Category: Zhang H]]
-	[[Category: H~~. pylori]]~~	+
-	~~[[Category: Motility]]~~	+
-	~~[[Category: Transferase-motor protein complex~~]]	+

OCA at 07:41, 29 November 2017

OCA — Wed, 29 Nov 2017 07:41:22 GMT

←Older revision		Revision as of 07:41, 29 November 2017
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	<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x0z]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x0z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X0Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">speE, HP_0832 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori]), HP_1031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0z OCA], [http://pdbe.org/5x0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x0z RCSB], [http://www.ebi.ac.uk/pdbsum/5x0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0z OCA], [http://pdbe.org/5x0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x0z RCSB], [http://www.ebi.ac.uk/pdbsum/5x0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0z ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter pylori]]
	[[Category: Au, S W.N]]		[[Category: Au, S W.N]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]

OCA at 05:33, 6 October 2017

OCA — Fri, 06 Oct 2017 05:33:49 GMT

←Older revision		Revision as of 05:33, 6 October 2017
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-	==~~motor regulator~~ complex==	+	==Crystal structure of FliM-SpeE complex from H. pylori==
	<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 10:42, 13 September 2017

OCA — Wed, 13 Sep 2017 10:42:45 GMT

←Older revision		Revision as of 10:42, 13 September 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5x0z is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==motor regulator complex==
		+	<StructureSection load='5x0z' size='340' side='right' caption='[[5x0z]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x0z]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X0Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0z OCA], [http://pdbe.org/5x0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x0z RCSB], [http://www.ebi.ac.uk/pdbsum/5x0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPEE_HELPY SPEE_HELPY]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor.<ref>PMID:16009566</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The flagellar motor is an important virulence factor in infection by many bacterial pathogens. Motor function can be modulated by chemotactic proteins and recently-appreciated proteins that are not part of the flagellar or chemotaxis systems. How these latter proteins affect flagellar activity is not fully understood. Here, we identified spermidine synthase SpeE as an interacting partner of switch protein FliM in Helicobacter pylori using pull-down assay and mass spectrometry. To understand how SpeE contributes to flagellar motility, a speE-null mutant was generated and its motility behavior was evaluated. We found that deletion of SpeE did not affect flagellar formation, but induced clockwise rotation bias. We further determined the crystal structure of the FliM-SpeE complex at 2.7 A resolution. SpeE dimer binds to FliM with micromolar binding affinity, and their interaction is mediated through the beta1' and beta2' region of FliM middle domain. The FliM-SpeE binding interface partially overlaps with the FliM surface that interacts with FliG and is essential for proper flagellar rotational switching. By a combination of protein sequence conservation analysis and pull-down assays using FliM and SpeE orthologues in E. coli, our data suggest that FliM-SpeE association is unique to Helicobacter species. This article is protected by copyright. All rights reserved.

-	~~Authors: Zhang~~, H., Au, W.N.S.	+	A putative spermidine synthase interacts with flagellar switch protein FliM and regulates motility in Helicobacter pylori.,Zhang H, Lam KH, Lam WWL, Wong SYY, Chan VSF, Au SWN Mol Microbiol. 2017 Sep 4. doi: 10.1111/mmi.13829. PMID:28868744<ref>PMID:28868744</ref>

-	~~Description~~: ~~motor regulator complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x0z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Au, S W.N]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]
-	[[Category: ~~Au, W~~.~~N.S~~]]	+	[[Category: Flagellar motor]]
		+	[[Category: H. pylori]]
		+	[[Category: Motility]]
		+	[[Category: Transferase-motor protein complex]]

OCA at 15:33, 9 March 2017

OCA — Thu, 09 Mar 2017 15:33:31 GMT

←Older revision		Revision as of 15:33, 9 March 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x0z is ON HOLD	+	The entry 5x0z is ON HOLD until Paper Publication

-	Authors: ~~ZHANG Huawei~~ , Au ~~Wing Ngor Shannon~~	+	Authors: Zhang, H., Au, W.N.S.

	Description: motor regulator complex		Description: motor regulator complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zhang ~~Huawei~~ , Au ~~Wing Ngor Shannon~~]]	+	[[Category: Zhang, H]]
		+	[[Category: Au, W.N.S]]

OCA: Protected "5x0z" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2017 17:09:47 GMT

Protected "5x0z" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:09, 1 February 2017

OCA: New page: '''Unreleased structure''' The entry 5x0z is ON HOLD Authors: ZHANG Huawei , Au Wing Ngor Shannon Description: motor regulator complex Category: Unreleased Structures [[Category: Z...

OCA — Wed, 01 Feb 2017 17:09:45 GMT

New page: '''Unreleased structure''' The entry 5x0z is ON HOLD Authors: ZHANG Huawei , Au Wing Ngor Shannon Description: motor regulator complex Category: Unreleased Structures [[Category: Z...

New page

'''Unreleased structure'''

The entry 5x0z is ON HOLD

Authors: ZHANG Huawei , Au Wing Ngor Shannon

Description: motor regulator complex
[[Category: Unreleased Structures]]
[[Category: Zhang Huawei , Au Wing Ngor Shannon]]