5x15 - Revision history

OCA at 09:19, 20 March 2024

OCA — Wed, 20 Mar 2024 09:19:36 GMT

←Older revision		Revision as of 09:19, 20 March 2024
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	<StructureSection load='5x15' size='340' side='right'caption='[[5x15]], [[Resolution\|resolution]] 3.09Å' scene=''>		<StructureSection load='5x15' size='340' side='right'caption='[[5x15]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x15]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strco Strco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x15]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X15 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SCO7163 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=100226 STRCO])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.094Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x15 OCA], [~~http~~://pdbe.org/5x15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x15 OCA], [https://pdbe.org/5x15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x15 RCSB], [https://www.ebi.ac.uk/pdbsum/5x15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x15 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9FBS9_STRCO Q9FBS9_STRCO]
-	Bacterial ribonuclease E (RNase E) plays a crucial role in the processing and decay of RNAs. A small protein named RraA negatively regulates the activity of RNase E via protein-protein interaction in various bacteria. Recently, RraAS1 and RraAS2, which are functional homologs of RraA from Escherichia coli, were identified in the Gram-positive species Streptomyces coelicolor. RraAS1 and RraAS2 inhibit RNase ES ribonuclease activity in S. coelicolor. RraAS1 and RraAS2 have a C-terminal extension region unlike typical bacterial RraA proteins. In this study, we present the crystal structure of RraAS2, exhibiting a hexamer arranged in a dimer of trimers, consistent with size exclusion chromatographic results. Importantly, the C-terminal extension region formed a long alpha-helix at the junction of the neighboring subunit, which is similar to the trimeric RraA orthologs from Saccharomyces cerevisiae. Truncation of the C-terminal extension region resulted in loss of RNase ES inhibition, demonstrating its crucial role. Our findings present the first bacterial RraA that has a hexameric assembly with a C-terminal extension alpha-helical region, which plays an essential role in the regulation of RNase ES activity in S. coelicolor.	+
-		+
-	~~Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase E inhibitor RraA protein family.,Park N, Heo J, Song S, Jo I, Lee K, Ha NC J Microbiol. 2017 May;55(5)~~:~~388-395. doi: 10.1007~~/~~s12275-017-7053-8. Epub 2017, Apr 29. PMID:28455590<ref>PMID:28455590<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5x15" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Ha N-C]]
-	[[Category: Ha, N C]]	+	[[Category: Jo I]]
-	[[Category: Jo, I]]	+	[[Category: Park N]]
-	[[Category: Park, N]]	+
-	~~[[Category: Rnase es inhibitor]]~~	+
-	~~[[Category: Rraa protein family]]~~	+
-	~~[[Category: Rraas2]]~~	+
-	~~[[Category: Streptomyces coelicolor]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transferase inhibitor~~]]	+

OCA at 07:21, 7 October 2020

OCA — Wed, 07 Oct 2020 07:21:37 GMT

←Older revision		Revision as of 07:21, 7 October 2020
Line 1:		Line 1:

	==Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase ES inhibitor RraA protein family==		==Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase ES inhibitor RraA protein family==
-	<StructureSection load='5x15' size='340' side='right' caption='[[5x15]], [[Resolution\|resolution]] 3.09Å' scene=''>	+	<StructureSection load='5x15' size='340' side='right'caption='[[5x15]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x15]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X15 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x15]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X15 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X15 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x15 OCA], [http://pdbe.org/5x15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x15 RCSB], [http://www.ebi.ac.uk/pdbsum/5x15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x15 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO7163 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x15 OCA], [http://pdbe.org/5x15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x15 RCSB], [http://www.ebi.ac.uk/pdbsum/5x15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x15 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Strco]]
	[[Category: Ha, N C]]		[[Category: Ha, N C]]
	[[Category: Jo, I]]		[[Category: Jo, I]]

OCA at 15:42, 17 May 2017

OCA — Wed, 17 May 2017 15:42:49 GMT

←Older revision		Revision as of 15:42, 17 May 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x15 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase ES inhibitor RraA protein family==
		+	<StructureSection load='5x15' size='340' side='right' caption='[[5x15]], [[Resolution\|resolution]] 3.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x15]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X15 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x15 OCA], [http://pdbe.org/5x15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x15 RCSB], [http://www.ebi.ac.uk/pdbsum/5x15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x15 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial ribonuclease E (RNase E) plays a crucial role in the processing and decay of RNAs. A small protein named RraA negatively regulates the activity of RNase E via protein-protein interaction in various bacteria. Recently, RraAS1 and RraAS2, which are functional homologs of RraA from Escherichia coli, were identified in the Gram-positive species Streptomyces coelicolor. RraAS1 and RraAS2 inhibit RNase ES ribonuclease activity in S. coelicolor. RraAS1 and RraAS2 have a C-terminal extension region unlike typical bacterial RraA proteins. In this study, we present the crystal structure of RraAS2, exhibiting a hexamer arranged in a dimer of trimers, consistent with size exclusion chromatographic results. Importantly, the C-terminal extension region formed a long alpha-helix at the junction of the neighboring subunit, which is similar to the trimeric RraA orthologs from Saccharomyces cerevisiae. Truncation of the C-terminal extension region resulted in loss of RNase ES inhibition, demonstrating its crucial role. Our findings present the first bacterial RraA that has a hexameric assembly with a C-terminal extension alpha-helical region, which plays an essential role in the regulation of RNase ES activity in S. coelicolor.

-	~~Authors: Park~~, N., Jo, I., Ha, N.-C.	+	Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase E inhibitor RraA protein family.,Park N, Heo J, Song S, Jo I, Lee K, Ha NC J Microbiol. 2017 May;55(5):388-395. doi: 10.1007/s12275-017-7053-8. Epub 2017, Apr 29. PMID:28455590<ref>PMID:28455590</ref>

-	~~Description: Crystal structure of Streptomyces coelicolor RraAS2~~, ~~an unusual member~~ of the ~~RNase ES inhibitor RraA protein family~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Ha, N.-C]]	+	<div class="pdbe-citations 5x15" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ha, N C]]
	[[Category: Jo, I]]		[[Category: Jo, I]]
	[[Category: Park, N]]		[[Category: Park, N]]
		+	[[Category: Rnase es inhibitor]]
		+	[[Category: Rraa protein family]]
		+	[[Category: Rraas2]]
		+	[[Category: Streptomyces coelicolor]]
		+	[[Category: Transferase]]
		+	[[Category: Transferase inhibitor]]

OCA at 20:04, 15 March 2017

OCA — Wed, 15 Mar 2017 20:04:14 GMT

←Older revision		Revision as of 20:04, 15 March 2017
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ha, N.-C]]		[[Category: Ha, N.-C]]
-	[[Category: Park, N]]
	[[Category: Jo, I]]		[[Category: Jo, I]]
		+	[[Category: Park, N]]

OCA at 15:33, 9 March 2017

OCA — Thu, 09 Mar 2017 15:33:52 GMT

←Older revision		Revision as of 15:33, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x15 is ON HOLD	+	The entry 5x15 is ON HOLD until Paper Publication

	Authors: Park, N., Jo, I., Ha, N.-C.		Authors: Park, N., Jo, I., Ha, N.-C.

OCA: Protected "5x15" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2017 17:10:09 GMT

Protected "5x15" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:10, 1 February 2017

OCA: New page: '''Unreleased structure''' The entry 5x15 is ON HOLD Authors: Park, N., Jo, I., Ha, N.-C. Description: Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNa...

OCA — Wed, 01 Feb 2017 17:10:07 GMT

New page: '''Unreleased structure''' The entry 5x15 is ON HOLD Authors: Park, N., Jo, I., Ha, N.-C. Description: Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNa...

New page

'''Unreleased structure'''

The entry 5x15 is ON HOLD

Authors: Park, N., Jo, I., Ha, N.-C.

Description: Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase ES inhibitor RraA protein family
[[Category: Unreleased Structures]]
[[Category: Ha, N.-C]]
[[Category: Park, N]]
[[Category: Jo, I]]