5x1e - Revision history

OCA at 09:19, 20 March 2024

OCA — Wed, 20 Mar 2024 09:19:42 GMT

←Older revision		Revision as of 09:19, 20 March 2024
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	<StructureSection load='5x1e' size='340' side='right'caption='[[5x1e]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5x1e' size='340' side='right'caption='[[5x1e]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x1e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X1E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x1e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X1E FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~5x1h~~\|~~5x1h~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.999Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1e OCA], [~~http~~://pdbe.org/5x1e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x1e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x1e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1e OCA], [https://pdbe.org/5x1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x1e RCSB], [https://www.ebi.ac.uk/pdbsum/5x1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1e ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/ICMS_LEGPH ICMS_LEGPH] Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:15661013, PubMed:18069892, PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). The IcmS/IcmW protein complex plays an important role in protein translocation by interacting with multiple Dot/Icm effector proteins to facilitate their translocation into host cells (PubMed:15661013, PubMed:18069892). Interaction promotes conformational changes in the effector protein, which may facilitate display of a C-terminal translocation signal (PubMed:18069892). May maintain the substrates in a translocation competent form (PubMed:23028312). Required for intracellular growth in host cells, replicative phagosome formation and phagosome trafficking (PubMed:11115108). IcmS is required for IcmW stability (PubMed:15661013, PubMed:29203674).<ref>PMID:11115108</ref> <ref>PMID:15661013</ref> <ref>PMID:17040490</ref> <ref>PMID:18069892</ref> <ref>PMID:22694730</ref> <ref>PMID:23028312</ref> <ref>PMID:29203674</ref> <ref>PMID:32513920</ref>
-	~~Many bacteria, including Legionella pneumophila, rely on~~ the type IV secretion system to ~~translocate a repertoire of~~ effector proteins into ~~the hosts for their survival and growth. Type IV coupling protein~~ (~~T4CP~~) ~~is a hexameric ATPase that links translocating substrates to the transenvelope secretion conduit~~. ~~Yet, how~~ a ~~large number of~~ effector proteins ~~are selectively recruited~~ and ~~processed by T4CPs remains enigmatic. DotL,~~ the ~~T4CP of L. pneumophila~~, ~~contains an ATPase domain and a C-terminal extension whose function is unknown~~. ~~Unlike T4CPs involved~~ in ~~plasmid DNA~~ translocation~~, DotL appeared to function~~ by ~~forming a multiprotein complex~~ with ~~four other~~ proteins~~. Here~~, ~~we show that~~ the C-terminal ~~extension of DotL interacts with DotN, IcmS, IcmW and an additionally identified subunit LvgA, and that this pentameric assembly binds Legionella effector proteins~~. ~~We determined~~ the ~~crystal structure of this assembly and built an architecture of the T4CP holocomplex by combining~~ a ~~homology model of the ATPase domain of DotL~~. ~~The holocomplex is a hexamer of a bipartite structure composed of a membrane-proximal ATPase domain~~ and ~~a membrane-distal substrate-recognition assembly~~. ~~The presented information demonstrates the architecture and functional dissection of the multiprotein T4CP complexes and provides important insights into their substrate recruitment and processing.~~	+
-		+
-	~~Architecture of the type IV coupling protein complex of Legionella pneumophila.,Kwak MJ, Kim JD, Kim H, Kim C, Bowman JW, Kim S, Joo K, Lee J, Jin KS, Kim YG, Lee NK~~, ~~Jung JU, Oh BH Nat Microbiol. 2017 Jul 17;2~~:~~17114~~. ~~doi~~: ~~10.1038~~/~~nmicrobiol.2017.114.~~ PMID:~~28714967~~<ref>PMID:~~28714967~~</ref>	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 5x1e" style="background-color~~:~~#fffaf0;"~~></~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kim, J D~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-	[[Category: ~~Kwak, M J~~]]	+	[[Category: Kim JD]]
-	[[Category: ~~Oh, B H~~]]	+	[[Category: Kwak MJ]]
-	[[Category: ~~Coupling protein complex]]~~	+	[[Category: Oh BH]]
-	~~[[Category: Effector translocation]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Type iv secretion system~~]]	+

OCA at 07:21, 7 October 2020

OCA — Wed, 07 Oct 2020 07:21:46 GMT

←Older revision		Revision as of 07:21, 7 October 2020
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	==Structure of DotL(656-783)-IcmS-IcmW derived from Legionella pneumophila==		==Structure of DotL(656-783)-IcmS-IcmW derived from Legionella pneumophila==
-	<StructureSection load='5x1e' size='340' side='right' caption='[[5x1e]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5x1e' size='340' side='right'caption='[[5x1e]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x1e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X1E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x1e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X1E FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1h\|5x1h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1h\|5x1h]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1e OCA], [http://pdbe.org/5x1e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1e RCSB], [http://www.ebi.ac.uk/pdbsum/5x1e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1e OCA], [http://pdbe.org/5x1e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1e RCSB], [http://www.ebi.ac.uk/pdbsum/5x1e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1e ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kim, J D]]		[[Category: Kim, J D]]
	[[Category: Kwak, M J]]		[[Category: Kwak, M J]]

OCA at 04:06, 4 August 2017

OCA — Fri, 04 Aug 2017 04:06:12 GMT

←Older revision		Revision as of 04:06, 4 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5x1e is ~~ON HOLD until Paper~~ Publication	+	==Structure of DotL(656-783)-IcmS-IcmW derived from Legionella pneumophila==
		+	<StructureSection load='5x1e' size='340' side='right' caption='[[5x1e]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x1e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X1E FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1h\|5x1h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1e OCA], [http://pdbe.org/5x1e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1e RCSB], [http://www.ebi.ac.uk/pdbsum/5x1e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1e ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many bacteria, including Legionella pneumophila, rely on the type IV secretion system to translocate a repertoire of effector proteins into the hosts for their survival and growth. Type IV coupling protein (T4CP) is a hexameric ATPase that links translocating substrates to the transenvelope secretion conduit. Yet, how a large number of effector proteins are selectively recruited and processed by T4CPs remains enigmatic. DotL, the T4CP of L. pneumophila, contains an ATPase domain and a C-terminal extension whose function is unknown. Unlike T4CPs involved in plasmid DNA translocation, DotL appeared to function by forming a multiprotein complex with four other proteins. Here, we show that the C-terminal extension of DotL interacts with DotN, IcmS, IcmW and an additionally identified subunit LvgA, and that this pentameric assembly binds Legionella effector proteins. We determined the crystal structure of this assembly and built an architecture of the T4CP holocomplex by combining a homology model of the ATPase domain of DotL. The holocomplex is a hexamer of a bipartite structure composed of a membrane-proximal ATPase domain and a membrane-distal substrate-recognition assembly. The presented information demonstrates the architecture and functional dissection of the multiprotein T4CP complexes and provides important insights into their substrate recruitment and processing.

-	~~Authors~~:	+	Architecture of the type IV coupling protein complex of Legionella pneumophila.,Kwak MJ, Kim JD, Kim H, Kim C, Bowman JW, Kim S, Joo K, Lee J, Jin KS, Kim YG, Lee NK, Jung JU, Oh BH Nat Microbiol. 2017 Jul 17;2:17114. doi: 10.1038/nmicrobiol.2017.114. PMID:28714967<ref>PMID:28714967</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x1e" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kim, J D]]
		+	[[Category: Kwak, M J]]
		+	[[Category: Oh, B H]]
		+	[[Category: Coupling protein complex]]
		+	[[Category: Effector translocation]]
		+	[[Category: Protein transport]]
		+	[[Category: Type iv secretion system]]

OCA at 15:34, 9 March 2017

OCA — Thu, 09 Mar 2017 15:34:40 GMT

←Older revision		Revision as of 15:34, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x1e is ON HOLD	+	The entry 5x1e is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5x1e" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2017 17:10:44 GMT

Protected "5x1e" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:10, 1 February 2017

OCA: New page: '''Unreleased structure''' The entry 5x1e is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Feb 2017 17:10:43 GMT

New page: '''Unreleased structure''' The entry 5x1e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5x1e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]