5x1y - Revision history

OCA at 07:53, 22 November 2023

OCA — Wed, 22 Nov 2023 07:53:06 GMT

←Older revision		Revision as of 07:53, 22 November 2023
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	==Structure of mercuric reductase from Lysinibacillus sphaericus==		==Structure of mercuric reductase from Lysinibacillus sphaericus==
-	<StructureSection load='5x1y' size='340' side='right' caption='[[5x1y]], [[Resolution\|resolution]] 3.48Å' scene=''>	+	<StructureSection load='5x1y' size='340' side='right'caption='[[5x1y]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x1y]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_14577 Atcc 14577~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x1y]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X1Y FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.48Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">merA ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1421 ATCC 14577])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Mercury(II)_reductase Mercury(II) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.1.1 1.16.1.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1y OCA], [https://pdbe.org/5x1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x1y RCSB], [https://www.ebi.ac.uk/pdbsum/5x1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1y ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1y OCA], [~~http~~://pdbe.org/5x1y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x1y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x1y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1y ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D9J041_LYSSH D9J041_LYSSH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 14577~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Khan, F~~]]	+	[[Category: Lysinibacillus sphaericus]]
-	[[Category: ~~Suguna, K~~]]	+	[[Category: Khan F]]
-	[[Category: ~~Fad]]~~	+	[[Category: Suguna K]]
-	~~[[Category: Mera]]~~	+
-	~~[[Category: Mercuric reductase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:52, 6 December 2017

OCA — Wed, 06 Dec 2017 07:52:18 GMT

←Older revision		Revision as of 07:52, 6 December 2017
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	<StructureSection load='5x1y' size='340' side='right' caption='[[5x1y]], [[Resolution\|resolution]] 3.48Å' scene=''>		<StructureSection load='5x1y' size='340' side='right' caption='[[5x1y]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x1y]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x1y]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X1Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">merA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1421 ATCC 14577])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mercury(II)_reductase Mercury(II) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.1.1 1.16.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mercury(II)_reductase Mercury(II) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.1.1 1.16.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1y OCA], [http://pdbe.org/5x1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1y RCSB], [http://www.ebi.ac.uk/pdbsum/5x1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1y OCA], [http://pdbe.org/5x1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1y RCSB], [http://www.ebi.ac.uk/pdbsum/5x1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1y ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 14577]]
	[[Category: Khan, F]]		[[Category: Khan, F]]
	[[Category: Suguna, K]]		[[Category: Suguna, K]]

OCA at 09:12, 4 October 2017

OCA — Wed, 04 Oct 2017 09:12:42 GMT

←Older revision		Revision as of 09:12, 4 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x1y is ~~ON HOLD until Paper~~ Publication	+	==Structure of mercuric reductase from Lysinibacillus sphaericus==
		+	<StructureSection load='5x1y' size='340' side='right' caption='[[5x1y]], [[Resolution\|resolution]] 3.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x1y]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X1Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mercury(II)_reductase Mercury(II) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.1.1 1.16.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x1y OCA], [http://pdbe.org/5x1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x1y RCSB], [http://www.ebi.ac.uk/pdbsum/5x1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x1y ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In response to the widespread presence of inorganic Hg in the environment, bacteria have evolved resistance systems with mercuric reductase (MerA) as the key enzyme. MerA enzymes have still not been well characterized from gram positive bacteria. Current study reports physico-chemical, kinetic and structural characterization of MerA from a multiple heavy metal resistant strain of Lysinibacillus sphaericus, and discusses its implications in bioremediation application. The enzyme was homodimeric with subunit molecular weight of about 60 kDa. The Km and Vmax were found to be 32 microM of HgCl2 and 18 units/mg respectively. The enzyme activity was enhanced by beta-mercaptoethanol and NaCl up to concentrations of 500 microM and 100 mM respectively, followed by inhibition at higher concentrations. The enzyme showed maximum activity in the pH range of 7-7.5 and temperature range of 25-50 degrees C, with melting temperature of 67 degrees C. Cu2+ exhibited pronounced inhibition of the enzyme with mixed inhibition pattern. The enzyme contained FAD as the prosthetic group and used NADPH as the preferred electron donor, but it showed slight activity with NADH as well. Structural characterization was carried out by circular dichroism spectrophotometry and X-ray crystallography. X-ray confirmed the homodimeric structure of enzyme and gave an insight on the residues involved in catalytic binding. In conclusion, the investigated enzyme showed higher catalytic efficiency, temperature stability and salt tolerance as compared to MerA enzymes from other mesophiles. Therefore, it is proposed to be a promising candidate for Hg2+ bioremediation.

-	~~Authors~~:	+	Structural and functional characterization of mercuric reductase from Lysinibacillus sphaericus strain G1.,Bafana A, Khan F, Suguna K Biometals. 2017 Sep 11. doi: 10.1007/s10534-017-0050-x. PMID:28894951<ref>PMID:28894951</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x1y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Khan, F]]
		+	[[Category: Suguna, K]]
		+	[[Category: Fad]]
		+	[[Category: Mera]]
		+	[[Category: Mercuric reductase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5x1y" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:35:44 GMT

Protected "5x1y" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:35, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x1y is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 15:35:42 GMT

New page: '''Unreleased structure''' The entry 5x1y is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5x1y is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]