5x3y - Revision history

OCA at 07:28, 1 May 2024

OCA — Wed, 01 May 2024 07:28:46 GMT

←Older revision		Revision as of 07:28, 1 May 2024
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	==Refined solution structure of musashi1 RBD2==		==Refined solution structure of musashi1 RBD2==
-	<StructureSection load='5x3y' size='340' side='right'caption='[[5x3y~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5x3y' size='340' side='right'caption='[[5x3y]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x3y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X3Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x3y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X3Y FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5x3z\|5x3z]]~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3y OCA], [~~http~~://pdbe.org/5x3y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x3y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x3y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3y OCA], [https://pdbe.org/5x3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x3y RCSB], [https://www.ebi.ac.uk/pdbsum/5x3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MSI1H_MOUSE MSI1H_MOUSE]] RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.<ref>PMID:11359897</ref> <ref>PMID:12407178</ref>	+	[https://www.uniprot.org/uniprot/MSI1H_MOUSE MSI1H_MOUSE] RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.<ref>PMID:11359897</ref> <ref>PMID:12407178</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Musashi-1 (Msi1) controls the maintenance of stem cells and tumorigenesis through binding to its target mRNAs and subsequent translational regulation. Msi1 has two RNA-binding domains (RBDs), RBD1 and RBD2, which recognize r(GUAG) and r(UAG), respectively. These minimal recognition sequences are connected by variable linkers in the Msi1 target mRNAs, however, the molecular mechanism by which Msi1 recognizes its targets is not yet understood. We previously determined the solution structure of the Msi1 RBD1:r(GUAGU) complex. Here, we determined the first structure of the RBD2:r(GUAGU) complex. The structure revealed that the central trinucleotide, r(UAG), is specifically recognized by the intermolecular hydrogen-bonding and aromatic stacking interactions. Importantly, the C-terminal region, which is disordered in the free form, took a certain conformation, resembling a helix. The observation of chemical shift perturbation and intermolecular NOEs, together with increases in the heteronuclear steady-state {(1)H}-(15)N NOE values on complex formation, indicated the involvement of the C-terminal region in RNA binding. On the basis of the two complex structures, we built a structural model of consecutive RBDs with r(UAGGUAG) containing both minimal recognition sequences, which resulted in no steric hindrance. The model suggests recognition of variable lengths (n) of the linker up to n = 50 may be possible.	+
-		+
-	Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA.,Iwaoka R, Nagata T, Tsuda K, Imai T, Okano H, Kobayashi N, Katahira M Molecules. 2017 Jul 19;22(7). pii: molecules22071207. doi:, 10.3390/molecules22071207. PMID:28753936<ref>PMID:28753936</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5x3y" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 14:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Imai, T]]	+	[[Category: Mus musculus]]
-	[[Category: Iwaoka, R]]	+	[[Category: Imai T]]
-	[[Category: Katahira, M]]	+	[[Category: Iwaoka R]]
-	[[Category: Kobayashi, N]]	+	[[Category: Katahira M]]
-	[[Category: Nagata, T]]	+	[[Category: Kobayashi N]]
-	[[Category: Okano, H]]	+	[[Category: Nagata T]]
-	[[Category: Tsuda, K]]	+	[[Category: Okano H]]
-	~~[[Category: Rbd]]~~	+	[[Category: Tsuda K]]
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna-binding protein]]~~	+
-	~~[[Category: Rrm~~]]	+

OCA at 07:24, 7 October 2020

OCA — Wed, 07 Oct 2020 07:24:26 GMT

←Older revision		Revision as of 07:24, 7 October 2020
Line 1:		Line 1:

	==Refined solution structure of musashi1 RBD2==		==Refined solution structure of musashi1 RBD2==
-	<StructureSection load='5x3y' size='340' side='right' caption='[[5x3y]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5x3y' size='340' side='right'caption='[[5x3y]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x3y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3Y OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X3Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x3y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X3Y FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x3z\|5x3z]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x3z\|5x3z]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3y OCA], [http://pdbe.org/5x3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x3y RCSB], [http://www.ebi.ac.uk/pdbsum/5x3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3y OCA], [http://pdbe.org/5x3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x3y RCSB], [http://www.ebi.ac.uk/pdbsum/5x3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Imai, T]]		[[Category: Imai, T]]
	[[Category: Iwaoka, R]]		[[Category: Iwaoka, R]]

OCA at 07:00, 13 December 2017

OCA — Wed, 13 Dec 2017 07:00:35 GMT

←Older revision		Revision as of 07:00, 13 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x3y is ~~ON HOLD~~ ~~until Feb 09 2019~~	+	==Refined solution structure of musashi1 RBD2==
		+	<StructureSection load='5x3y' size='340' side='right' caption='[[5x3y]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x3y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X3Y FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x3z\|5x3z]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3y OCA], [http://pdbe.org/5x3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x3y RCSB], [http://www.ebi.ac.uk/pdbsum/5x3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3y ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MSI1H_MOUSE MSI1H_MOUSE]] RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.<ref>PMID:11359897</ref> <ref>PMID:12407178</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Musashi-1 (Msi1) controls the maintenance of stem cells and tumorigenesis through binding to its target mRNAs and subsequent translational regulation. Msi1 has two RNA-binding domains (RBDs), RBD1 and RBD2, which recognize r(GUAG) and r(UAG), respectively. These minimal recognition sequences are connected by variable linkers in the Msi1 target mRNAs, however, the molecular mechanism by which Msi1 recognizes its targets is not yet understood. We previously determined the solution structure of the Msi1 RBD1:r(GUAGU) complex. Here, we determined the first structure of the RBD2:r(GUAGU) complex. The structure revealed that the central trinucleotide, r(UAG), is specifically recognized by the intermolecular hydrogen-bonding and aromatic stacking interactions. Importantly, the C-terminal region, which is disordered in the free form, took a certain conformation, resembling a helix. The observation of chemical shift perturbation and intermolecular NOEs, together with increases in the heteronuclear steady-state {(1)H}-(15)N NOE values on complex formation, indicated the involvement of the C-terminal region in RNA binding. On the basis of the two complex structures, we built a structural model of consecutive RBDs with r(UAGGUAG) containing both minimal recognition sequences, which resulted in no steric hindrance. The model suggests recognition of variable lengths (n) of the linker up to n = 50 may be possible.

-	~~Authors~~:	+	Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA.,Iwaoka R, Nagata T, Tsuda K, Imai T, Okano H, Kobayashi N, Katahira M Molecules. 2017 Jul 19;22(7). pii: molecules22071207. doi:, 10.3390/molecules22071207. PMID:28753936<ref>PMID:28753936</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x3y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Imai, T]]
		+	[[Category: Iwaoka, R]]
		+	[[Category: Katahira, M]]
		+	[[Category: Kobayashi, N]]
		+	[[Category: Nagata, T]]
		+	[[Category: Okano, H]]
		+	[[Category: Tsuda, K]]
		+	[[Category: Rbd]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna-binding protein]]
		+	[[Category: Rrm]]

OCA at 12:14, 5 April 2017

OCA — Wed, 05 Apr 2017 12:14:35 GMT

←Older revision		Revision as of 12:14, 5 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x3y is ON HOLD	+	The entry 5x3y is ON HOLD until Feb 09 2019

	Authors:		Authors:

OCA: Protected "5x3y" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:43:02 GMT

Protected "5x3y" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:43, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x3y is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 15:43:01 GMT

New page: '''Unreleased structure''' The entry 5x3y is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5x3y is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]