5x42 - Revision history

OCA at 10:17, 27 March 2024

OCA — Wed, 27 Mar 2024 10:17:14 GMT

←Older revision		Revision as of 10:17, 27 March 2024
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	<StructureSection load='5x42' size='340' side='right'caption='[[5x42]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5x42' size='340' side='right'caption='[[5x42]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x42]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X42 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X42 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x42]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X42 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5x1u\|5x1u]], [[5x1h\|5x1h]], [[5x1e\|5x1e]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x42 OCA], [~~http~~://pdbe.org/5x42 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x42 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x42 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x42 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x42 OCA], [https://pdbe.org/5x42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x42 RCSB], [https://www.ebi.ac.uk/pdbsum/5x42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x42 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/DOTN_LEGPH DOTN_LEGPH] Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:32513920).<ref>PMID:17040490</ref> <ref>PMID:22694730</ref> <ref>PMID:32513920</ref>
-	~~Many bacteria, including Legionella pneumophila, rely on~~ the type IV secretion system ~~to translocate a repertoire of effector proteins into the hosts for their survival and growth. Type IV coupling protein~~ (~~T4CP~~) is ~~a hexameric ATPase that links translocating substrates~~ to ~~the transenvelope secretion conduit. Yet, how a large number of~~ effector proteins ~~are selectively recruited and processed by T4CPs remains enigmatic. DotL~~, ~~the T4CP of L. pneumophila~~, ~~contains an ATPase domain and a C-terminal extension whose function is unknown~~. ~~Unlike T4CPs involved in plasmid DNA translocation, DotL appeared to function by forming~~ a multiprotein complex with four other proteins. Here, we show that the C-terminal extension of DotL interacts with DotN, IcmS, IcmW and an additionally identified subunit LvgA, and that this pentameric assembly binds Legionella effector proteins~~. We determined the crystal structure of this assembly~~ and ~~built an architecture of~~ the T4CP holocomplex by combining a homology model of the ATPase domain of DotL. The holocomplex is a hexamer of a bipartite structure composed of a membrane-proximal ATPase domain and a membrane-distal substrate-recognition assembly. The presented information demonstrates the architecture and functional dissection of the multiprotein T4CP complexes and provides important insights into their substrate recruitment and processing.	+
-		+
-	Architecture of the type IV coupling protein complex of Legionella pneumophila.,Kwak MJ, Kim JD, Kim H, Kim C, Bowman JW, Kim S, Joo K, Lee J, Jin KS, Kim YG, Lee NK, Jung JU, Oh BH Nat Microbiol. 2017 Jul 17;2:~~17114~~. ~~doi: 10.1038/nmicrobiol.2017.114. PMID:28714967~~<ref>PMID:~~28714967~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 5x42" style="background-color:#fffaf0;"~~></~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kim, J D~~]]	+	[[Category: Legionella pneumophila]]
-	[[Category: ~~Kwak, M J~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-	[[Category: ~~Oh, B H~~]]	+	[[Category: Kim JD]]
-	[[Category: ~~Coupling protein complex~~]]	+	[[Category: Kwak MJ]]
-	[[Category: ~~Effector translocation]]~~	+	[[Category: Oh BH]]
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Type iv secretion system~~]]	+

OCA at 07:24, 7 October 2020

OCA — Wed, 07 Oct 2020 07:24:40 GMT

←Older revision		Revision as of 07:24, 7 October 2020
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	==Structure of DotL(590-659)-DotN derived from Legionella pneumophila==		==Structure of DotL(590-659)-DotN derived from Legionella pneumophila==
-	<StructureSection load='5x42' size='340' side='right' caption='[[5x42]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5x42' size='340' side='right'caption='[[5x42]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x42]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X42 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X42 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x42]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X42 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X42 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1u\|5x1u]], [[5x1h\|5x1h]], [[5x1e\|5x1e]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1u\|5x1u]], [[5x1h\|5x1h]], [[5x1e\|5x1e]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x42 OCA], [http://pdbe.org/5x42 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x42 RCSB], [http://www.ebi.ac.uk/pdbsum/5x42 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x42 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x42 OCA], [http://pdbe.org/5x42 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x42 RCSB], [http://www.ebi.ac.uk/pdbsum/5x42 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x42 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kim, J D]]		[[Category: Kim, J D]]
	[[Category: Kwak, M J]]		[[Category: Kwak, M J]]

OCA at 04:03, 4 August 2017

OCA — Fri, 04 Aug 2017 04:03:20 GMT

←Older revision		Revision as of 04:03, 4 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x42 is ~~ON HOLD until Paper~~ Publication	+	==Structure of DotL(590-659)-DotN derived from Legionella pneumophila==
		+	<StructureSection load='5x42' size='340' side='right' caption='[[5x42]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x42]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X42 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X42 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x1u\|5x1u]], [[5x1h\|5x1h]], [[5x1e\|5x1e]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x42 OCA], [http://pdbe.org/5x42 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x42 RCSB], [http://www.ebi.ac.uk/pdbsum/5x42 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x42 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many bacteria, including Legionella pneumophila, rely on the type IV secretion system to translocate a repertoire of effector proteins into the hosts for their survival and growth. Type IV coupling protein (T4CP) is a hexameric ATPase that links translocating substrates to the transenvelope secretion conduit. Yet, how a large number of effector proteins are selectively recruited and processed by T4CPs remains enigmatic. DotL, the T4CP of L. pneumophila, contains an ATPase domain and a C-terminal extension whose function is unknown. Unlike T4CPs involved in plasmid DNA translocation, DotL appeared to function by forming a multiprotein complex with four other proteins. Here, we show that the C-terminal extension of DotL interacts with DotN, IcmS, IcmW and an additionally identified subunit LvgA, and that this pentameric assembly binds Legionella effector proteins. We determined the crystal structure of this assembly and built an architecture of the T4CP holocomplex by combining a homology model of the ATPase domain of DotL. The holocomplex is a hexamer of a bipartite structure composed of a membrane-proximal ATPase domain and a membrane-distal substrate-recognition assembly. The presented information demonstrates the architecture and functional dissection of the multiprotein T4CP complexes and provides important insights into their substrate recruitment and processing.

-	~~Authors:~~ Kwak, ~~M.J.~~, Kim, J~~.D.~~, Oh, B.H.	+	Architecture of the type IV coupling protein complex of Legionella pneumophila.,Kwak MJ, Kim JD, Kim H, Kim C, Bowman JW, Kim S, Joo K, Lee J, Jin KS, Kim YG, Lee NK, Jung JU, Oh BH Nat Microbiol. 2017 Jul 17;2:17114. doi: 10.1038/nmicrobiol.2017.114. PMID:28714967<ref>PMID:28714967</ref>

-	~~Description: Structure~~ of ~~DotL(590~~-~~659)~~-~~DotN derived from Legionella pneumophila~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Oh, B.H]]	+	<div class="pdbe-citations 5x42" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kwak, M.J~~]]	+	== References ==
-	[[Category: ~~Kim, J.D~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kim, J D]]
		+	[[Category: Kwak, M J]]
		+	[[Category: Oh, B H]]
		+	[[Category: Coupling protein complex]]
		+	[[Category: Effector translocation]]
		+	[[Category: Protein transport]]
		+	[[Category: Type iv secretion system]]

OCA at 20:09, 15 March 2017

OCA — Wed, 15 Mar 2017 20:09:39 GMT

←Older revision		Revision as of 20:09, 15 March 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x42 is ON HOLD	+	The entry 5x42 is ON HOLD until Paper Publication

	Authors: Kwak, M.J., Kim, J.D., Oh, B.H.		Authors: Kwak, M.J., Kim, J.D., Oh, B.H.

OCA: Protected "5x42" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:43:14 GMT

Protected "5x42" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:43, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x42 is ON HOLD Authors: Kwak, M.J., Kim, J.D., Oh, B.H. Description: Structure of DotL(590-659)-DotN derived from Legionella pneumophila [[Categor...

OCA — Thu, 09 Mar 2017 15:43:13 GMT

New page: '''Unreleased structure''' The entry 5x42 is ON HOLD Authors: Kwak, M.J., Kim, J.D., Oh, B.H. Description: Structure of DotL(590-659)-DotN derived from Legionella pneumophila [[Categor...

New page

'''Unreleased structure'''

The entry 5x42 is ON HOLD

Authors: Kwak, M.J., Kim, J.D., Oh, B.H.

Description: Structure of DotL(590-659)-DotN derived from Legionella pneumophila
[[Category: Unreleased Structures]]
[[Category: Oh, B.H]]
[[Category: Kwak, M.J]]
[[Category: Kim, J.D]]