5x4s - Revision history

OCA at 07:25, 7 October 2020

OCA — Wed, 07 Oct 2020 07:25:22 GMT

←Older revision		Revision as of 07:25, 7 October 2020
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	==Structure of the N-terminal domain (NTD)of SARS-CoV spike protein==		==Structure of the N-terminal domain (NTD)of SARS-CoV spike protein==
-	<StructureSection load='5x4s' size='340' side='right' caption='[[5x4s]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='5x4s' size='340' side='right'caption='[[5x4s]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x4s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4S OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X4S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x4s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X4S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4r\|5x4r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4r\|5x4r]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4s OCA], [http://pdbe.org/5x4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4s RCSB], [http://www.ebi.ac.uk/pdbsum/5x4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4s OCA], [http://pdbe.org/5x4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4s RCSB], [http://www.ebi.ac.uk/pdbsum/5x4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5x4s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5x4s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sandbox 3001\|Sandbox 3001]]
		+	*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]
	[[Category: Qi, J]]		[[Category: Qi, J]]

OCA at 12:59, 4 May 2017

OCA — Thu, 04 May 2017 12:59:08 GMT

←Older revision		Revision as of 12:59, 4 May 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x4s is ~~ON HOLD~~	+	==Structure of the N-terminal domain (NTD)of SARS-CoV spike protein==
		+	<StructureSection load='5x4s' size='340' side='right' caption='[[5x4s]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x4s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X4S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4r\|5x4r]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4s OCA], [http://pdbe.org/5x4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4s RCSB], [http://www.ebi.ac.uk/pdbsum/5x4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4s ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.

-	~~Authors~~:	+	Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains.,Yuan Y, Cao D, Zhang Y, Ma J, Qi J, Wang Q, Lu G, Wu Y, Yan J, Shi Y, Zhang X, Gao GF Nat Commun. 2017 Apr 10;8:15092. doi: 10.1038/ncomms15092. PMID:28393837<ref>PMID:28393837</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x4s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gao, G F]]
		+	[[Category: Qi, J]]
		+	[[Category: Shi, Y]]
		+	[[Category: Yuan, Y]]
		+	[[Category: Zhang, Y]]
		+	[[Category: N-terminal domain]]
		+	[[Category: Sars-cov]]
		+	[[Category: Spike]]
		+	[[Category: Viral protein]]

OCA: Protected "5x4s" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:45:08 GMT

Protected "5x4s" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:45, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x4s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 15:45:07 GMT

New page: '''Unreleased structure''' The entry 5x4s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5x4s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]