5x4u - Revision history

OCA at 10:17, 27 March 2024

OCA — Wed, 27 Mar 2024 10:17:17 GMT

←Older revision		Revision as of 10:17, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='5x4u' size='340' side='right'caption='[[5x4u]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5x4u' size='340' side='right'caption='[[5x4u]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x4u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~9cyan 9cyan~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x4u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanobacteria Cyanobacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X4U FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5x4t\|5x4t]], [[5x4v\|5x4v]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [~~http~~://pdbe.org/5x4u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [https://pdbe.org/5x4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [https://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/K9TLZ5_9CYAN K9TLZ5_9CYAN]
-	The photoactivated adenylate cyclase (PAC) from the photosynthetic cyanobacterium Oscillatoria acuminata (OaPAC) detects light through a flavin chromophore within the N-terminal BLUF domain. BLUF domains have been found in a number of different light-activated proteins, but with different relative orientations. The two BLUF domains of OaPAC are found in close contact with each other, forming a coiled coil at their interface. Crystallization does not impede the activity switching of the enzyme, but flash cooling the crystals to cryogenic temperatures prevents the signature spectral changes that occur on photoactivation/deactivation. High-resolution crystallographic analysis of OaPAC in the fully activated state has been achieved by cryocooling the crystals immediately after light exposure. Comparison of the isomorphous light- and dark-state structures shows that the active site undergoes minimal changes, yet enzyme activity may increase up to 50-fold, depending on conditions. The OaPAC models will assist the development of simple, direct means to raise the cyclic AMP levels of living cells by light, and other tools for optogenetics.	+
-		+
-	~~Molecular mechanism of photoactivation of a light-regulated adenylate cyclase.,Ohki M, Sato-Tomita A, Matsunaga S, Iseki M, Tame JRH, Shibayama N, Park SY Proc Natl Acad Sci U S A. 2017 Aug 8;114(32)~~:~~8562-8567. doi:, 10.1073~~/~~pnas.1704391114. Epub 2017 Jul 24. PMID:28739908<ref>PMID:28739908<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5x4u" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]		*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cyanobacteria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ohki, M]]	+	[[Category: Ohki M]]
-	[[Category: Park, S Y]]	+	[[Category: Park S-Y]]
-	~~[[Category: Allostery]]~~	+
-	~~[[Category: Bluf domain]]~~	+
-	~~[[Category: Camp]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Optogenetic]]~~	+
-	~~[[Category: Photoactivation~~]]	+

OCA at 07:25, 7 October 2020

OCA — Wed, 07 Oct 2020 07:25:22 GMT

←Older revision		Revision as of 07:25, 7 October 2020
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	==PAC from Oscillatoriaacuminata after 60 seconds photoactivation==		==PAC from Oscillatoriaacuminata after 60 seconds photoactivation==
-	<StructureSection load='5x4u' size='340' side='right' caption='[[5x4u]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5x4u' size='340' side='right'caption='[[5x4u]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/9cyan 9cyan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4U OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/9cyan 9cyan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X4U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4t\|5x4t]], [[5x4v\|5x4v]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4t\|5x4t]], [[5x4v\|5x4v]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [http://pdbe.org/5x4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [http://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [http://pdbe.org/5x4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [http://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5x4u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5x4u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ohki, M]]		[[Category: Ohki, M]]
	[[Category: Park, S Y]]		[[Category: Park, S Y]]

OCA at 07:52, 6 December 2017

OCA — Wed, 06 Dec 2017 07:52:45 GMT

←Older revision		Revision as of 07:52, 6 December 2017
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [http://pdbe.org/5x4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [http://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [http://pdbe.org/5x4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [http://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The photoactivated adenylate cyclase (PAC) from the photosynthetic cyanobacterium Oscillatoria acuminata (OaPAC) detects light through a flavin chromophore within the N-terminal BLUF domain. BLUF domains have been found in a number of different light-activated proteins, but with different relative orientations. The two BLUF domains of OaPAC are found in close contact with each other, forming a coiled coil at their interface. Crystallization does not impede the activity switching of the enzyme, but flash cooling the crystals to cryogenic temperatures prevents the signature spectral changes that occur on photoactivation/deactivation. High-resolution crystallographic analysis of OaPAC in the fully activated state has been achieved by cryocooling the crystals immediately after light exposure. Comparison of the isomorphous light- and dark-state structures shows that the active site undergoes minimal changes, yet enzyme activity may increase up to 50-fold, depending on conditions. The OaPAC models will assist the development of simple, direct means to raise the cyclic AMP levels of living cells by light, and other tools for optogenetics.
		+
		+	Molecular mechanism of photoactivation of a light-regulated adenylate cyclase.,Ohki M, Sato-Tomita A, Matsunaga S, Iseki M, Tame JRH, Shibayama N, Park SY Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8562-8567. doi:, 10.1073/pnas.1704391114. Epub 2017 Jul 24. PMID:28739908<ref>PMID:28739908</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5x4u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:58, 16 November 2017

OCA — Thu, 16 Nov 2017 04:58:15 GMT

←Older revision		Revision as of 04:58, 16 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x4u ~~is ON HOLD until Paper Publication~~	+	==PAC from Oscillatoriaacuminata after 60 seconds photoactivation==
-		+	<StructureSection load='5x4u' size='340' side='right' caption='[[5x4u]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~Authors~~: ~~Ohki, M~~.~~, Park, S~~.-Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5x4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/9cyan 9cyan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X4U FirstGlance]. <br>
-	~~Description~~: ~~PAC from Oscillatoriaacuminata after 60 seconds photoactivation~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4t\|5x4t]], [[5x4v\|5x4v]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x4u OCA], [http://pdbe.org/5x4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x4u RCSB], [http://www.ebi.ac.uk/pdbsum/5x4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x4u ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ohki, M]]		[[Category: Ohki, M]]
-	[[Category: Park, S.-Y]]	+	[[Category: Park, S Y]]
		+	[[Category: Allostery]]
		+	[[Category: Bluf domain]]
		+	[[Category: Camp]]
		+	[[Category: Lyase]]
		+	[[Category: Optogenetic]]
		+	[[Category: Photoactivation]]

OCA at 20:10, 15 March 2017

OCA — Wed, 15 Mar 2017 20:10:59 GMT

←Older revision		Revision as of 20:10, 15 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x4u is ON HOLD	+	The entry 5x4u is ON HOLD until Paper Publication

	Authors: Ohki, M., Park, S.-Y.		Authors: Ohki, M., Park, S.-Y.

OCA: Protected "5x4u" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:45:15 GMT

Protected "5x4u" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:45, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x4u is ON HOLD Authors: Ohki, M., Park, S.-Y. Description: PAC from Oscillatoriaacuminata after 60 seconds photoactivation [[Category: Unreleased ...

OCA — Thu, 09 Mar 2017 15:45:13 GMT

New page: '''Unreleased structure''' The entry 5x4u is ON HOLD Authors: Ohki, M., Park, S.-Y. Description: PAC from Oscillatoriaacuminata after 60 seconds photoactivation [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 5x4u is ON HOLD

Authors: Ohki, M., Park, S.-Y.

Description: PAC from Oscillatoriaacuminata after 60 seconds photoactivation
[[Category: Unreleased Structures]]
[[Category: Ohki, M]]
[[Category: Park, S.-Y]]