5x51 - Revision history

OCA at 07:26, 7 October 2020

OCA — Wed, 07 Oct 2020 07:26:59 GMT

←Older revision		Revision as of 07:26, 7 October 2020
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	==RNA Polymerase II from Komagataella Pastoris (Type-3 crystal)==		==RNA Polymerase II from Komagataella Pastoris (Type-3 crystal)==
-	<StructureSection load='5x51' size='340' side='right' caption='[[5x51]], [[Resolution\|resolution]] 7.00Å' scene=''>	+	<StructureSection load='5x51' size='340' side='right'caption='[[5x51]], [[Resolution\|resolution]] 7.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x51]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Komagataella_phaffii_(strain_atcc_76273_/_cbs_7435_/_cect_11047_/_nrrl_y-11430_/_wegner_21-1) Komagataella phaffii (strain atcc 76273 / cbs 7435 / cect 11047 / nrrl y-11430 / wegner 21-1)~~] and [http://en.wikipedia.org/wiki/~~Komagataella_phaffii_(strain_gs115_/_atcc_20864) Komagataella phaffii (strain gs115 / atcc 20864)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X51 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X51 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x51]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Kompc Kompc] and [http://en.wikipedia.org/wiki/Kompg Kompg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X51 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X51 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4z\|5x4z]], [[5x50\|5x50]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4z\|5x4z]], [[5x50\|5x50]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x51 OCA], [http://pdbe.org/5x51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x51 RCSB], [http://www.ebi.ac.uk/pdbsum/5x51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x51 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x51 OCA], [http://pdbe.org/5x51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x51 RCSB], [http://www.ebi.ac.uk/pdbsum/5x51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x51 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5x51" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5x51" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
		+	[[Category: Kompc]]
		+	[[Category: Kompg]]
		+	[[Category: Large Structures]]
	[[Category: Ehara, H]]		[[Category: Ehara, H]]
	[[Category: Sekine, S]]		[[Category: Sekine, S]]

OCA at 15:45, 17 May 2017

OCA — Wed, 17 May 2017 15:45:39 GMT

←Older revision		Revision as of 15:45, 17 May 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5x51 is ~~ON HOLD until Paper~~ Publication	+	==RNA Polymerase II from Komagataella Pastoris (Type-3 crystal)==
		+	<StructureSection load='5x51' size='340' side='right' caption='[[5x51]], [[Resolution\|resolution]] 7.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x51]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_phaffii_(strain_atcc_76273_/_cbs_7435_/_cect_11047_/_nrrl_y-11430_/_wegner_21-1) Komagataella phaffii (strain atcc 76273 / cbs 7435 / cect 11047 / nrrl y-11430 / wegner 21-1)] and [http://en.wikipedia.org/wiki/Komagataella_phaffii_(strain_gs115_/_atcc_20864) Komagataella phaffii (strain gs115 / atcc 20864)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X51 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x4z\|5x4z]], [[5x50\|5x50]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x51 OCA], [http://pdbe.org/5x51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x51 RCSB], [http://www.ebi.ac.uk/pdbsum/5x51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x51 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/F2QPE6_KOMPC F2QPE6_KOMPC]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[PIRNR:PIRNR005586] [[http://www.uniprot.org/uniprot/C4QZQ7_KOMPG C4QZQ7_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU363031] [[http://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNA polymerase II (Pol II) is a 12-subunit protein complex that conducts the transcription of mRNA and some small RNAs. In this work, the crystal structure of Pol II from the methylotropic yeast Komagataella pastoris (Pichia pastoris) was determined. While the structure is highly homologous to that of Pol II from the budding yeast Saccharomyces cerevisiae, the stalk and clamp modules of the K. pastoris Pol II displayed large inward rotations, closing the central cleft to a greater extent than in the known S. cerevisiae Pol II structures. The conformational differences reflect the inherent flexibilities of the stalk and the clamp, as additional low-resolution structures of K. pastoris Pol II in different crystal forms revealed diverse stalk and clamp orientations. Comparisons with other eukaryotic/archaeal RNA polymerase structures in the Protein Data Bank revealed the distributions of the stalk and clamp orientations. The conformational plasticity should be essential for transcriptional functions and binding various regulatory factors.

-	~~Authors~~:	+	Crystal structure of RNA polymerase II from Komagataella pastoris.,Ehara H, Umehara T, Sekine SI, Yokoyama S Biochem Biophys Res Commun. 2017 May 27;487(2):230-235. doi:, 10.1016/j.bbrc.2017.04.039. Epub 2017 Apr 12. PMID:28412353<ref>PMID:28412353</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5x51" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA-directed RNA polymerase]]
		+	[[Category: Ehara, H]]
		+	[[Category: Sekine, S]]
		+	[[Category: Umehara, T]]
		+	[[Category: Yokoyama, S]]
		+	[[Category: Rna polymerase]]
		+	[[Category: Transcription]]
		+	[[Category: Transferase]]

OCA at 11:38, 10 May 2017

OCA — Wed, 10 May 2017 11:38:08 GMT

←Older revision		Revision as of 11:38, 10 May 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5x51 is ON HOLD	+	The entry 5x51 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5x51" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 15:45:36 GMT

Protected "5x51" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:45, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x51 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 15:45:35 GMT

New page: '''Unreleased structure''' The entry 5x51 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5x51 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]