5xa5 - Revision history

OCA at 10:17, 27 March 2024

OCA — Wed, 27 Mar 2024 10:17:46 GMT

←Older revision		Revision as of 10:17, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='5xa5' size='340' side='right'caption='[[5xa5]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5xa5' size='340' side='right'caption='[[5xa5]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Caeel Caeel~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XA5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XA5 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~hmp~~-1, ~~R13H4.4 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL~~]~~), hmp-2, K05C4~~.6 ~~([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=6239 CAEEL])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [~~http~~://pdbe.org/5xa5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [https://pdbe.org/5xa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [https://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref~~> [[http://www.uniprot.org/uniprot/HMP2_CAEEL HMP2_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation.<ref>PMID:9531567</ref>~~	+	[https://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Stable tissue integrity during embryonic development relies on the function of the cadherin.catenin complex (CCC). The Caenorhabditis elegans CCC is a useful paradigm for analyzing in vivo requirements for specific interactions among the core components of the CCC, and it provides a unique opportunity to examine evolutionarily conserved mechanisms that govern the interaction between alpha- and beta-catenin. HMP-1, unlike its mammalian homolog alpha-catenin, is constitutively monomeric, and its binding affinity for HMP-2/beta-catenin is higher than that of alpha-catenin for beta-catenin. A crystal structure shows that the HMP-1.HMP-2 complex forms a five-helical bundle structure distinct from the structure of the mammalian alpha-catenin.beta-catenin complex. Deletion analysis based on the crystal structure shows that the first helix of HMP-1 is necessary for binding HMP-2 avidly in vitro and for efficient recruitment of HMP-1 to adherens junctions in embryos. HMP-2 Ser-47 and Tyr-69 flank its binding interface with HMP-1, and we show that phosphomimetic mutations at these two sites decrease binding affinity of HMP-1 to HMP-2 by 40-100-fold in vitro. In vivo experiments using HMP-2 S47E and Y69E mutants showed that they are unable to rescue hmp-2(zu364) mutants, suggesting that phosphorylation of HMP-2 on Ser-47 and Tyr-69 could be important for regulating CCC formation in C. elegans Our data provide novel insights into how cadherin-dependent cell-cell adhesion is modulated in metazoans by conserved elements as well as features unique to specific organisms.	+
-		+
-	Cell-cell adhesion in metazoans relies on evolutionarily conserved features of the alpha-catenin.beta-catenin-binding interface.,Shao X, Kang H, Loveless T, Lee GR, Seok C, Weis WI, Choi HJ, Hardin J J Biol Chem. 2017 Oct 6;292(40):16477-16490. doi: 10.1074/jbc.M117.795567. Epub, 2017 Aug 25. PMID:28842483<ref>PMID:28842483</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5xa5" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caeel~~]]	+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Choi~~, H J~~]]	+	[[Category: Choi HJ]]
-	[[Category: Hardin, J]]	+	[[Category: Hardin J]]
-	[[Category: Kang, H]]	+	[[Category: Kang H]]
-	[[Category: Shao, X]]	+	[[Category: Shao X]]
-	[[Category: Weis~~, W I]]~~	+	[[Category: Weis WI]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Five-helix bundle~~]]	+

OCA at 12:40, 25 December 2019

OCA — Wed, 25 Dec 2019 12:40:54 GMT

←Older revision		Revision as of 12:40, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of HMP-1-HMP-2 complex==		==Crystal structure of HMP-1-HMP-2 complex==
-	<StructureSection load='5xa5' size='340' side='right' caption='[[5xa5]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5xa5' size='340' side='right'caption='[[5xa5]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XA5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XA5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [http://pdbe.org/5xa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [http://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hmp-1, R13H4.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), hmp-2, K05C4.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [http://pdbe.org/5xa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [http://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref> [[http://www.uniprot.org/uniprot/HMP2_CAEEL HMP2_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation.<ref>PMID:9531567</ref>		[[http://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref> [[http://www.uniprot.org/uniprot/HMP2_CAEEL HMP2_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation.<ref>PMID:9531567</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Stable tissue integrity during embryonic development relies on the function of the cadherin.catenin complex (CCC). The Caenorhabditis elegans CCC is a useful paradigm for analyzing in vivo requirements for specific interactions among the core components of the CCC, and it provides a unique opportunity to examine evolutionarily conserved mechanisms that govern the interaction between alpha- and beta-catenin. HMP-1, unlike its mammalian homolog alpha-catenin, is constitutively monomeric, and its binding affinity for HMP-2/beta-catenin is higher than that of alpha-catenin for beta-catenin. A crystal structure shows that the HMP-1.HMP-2 complex forms a five-helical bundle structure distinct from the structure of the mammalian alpha-catenin.beta-catenin complex. Deletion analysis based on the crystal structure shows that the first helix of HMP-1 is necessary for binding HMP-2 avidly in vitro and for efficient recruitment of HMP-1 to adherens junctions in embryos. HMP-2 Ser-47 and Tyr-69 flank its binding interface with HMP-1, and we show that phosphomimetic mutations at these two sites decrease binding affinity of HMP-1 to HMP-2 by 40-100-fold in vitro. In vivo experiments using HMP-2 S47E and Y69E mutants showed that they are unable to rescue hmp-2(zu364) mutants, suggesting that phosphorylation of HMP-2 on Ser-47 and Tyr-69 could be important for regulating CCC formation in C. elegans Our data provide novel insights into how cadherin-dependent cell-cell adhesion is modulated in metazoans by conserved elements as well as features unique to specific organisms.
		+
		+	Cell-cell adhesion in metazoans relies on evolutionarily conserved features of the alpha-catenin.beta-catenin-binding interface.,Shao X, Kang H, Loveless T, Lee GR, Seok C, Weis WI, Choi HJ, Hardin J J Biol Chem. 2017 Oct 6;292(40):16477-16490. doi: 10.1074/jbc.M117.795567. Epub, 2017 Aug 25. PMID:28842483<ref>PMID:28842483</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xa5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caeel]]
		+	[[Category: Large Structures]]
	[[Category: Choi, H J]]		[[Category: Choi, H J]]
	[[Category: Hardin, J]]		[[Category: Hardin, J]]

OCA at 14:59, 5 September 2017

OCA — Tue, 05 Sep 2017 14:59:51 GMT

←Older revision		Revision as of 14:59, 5 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xa5 is ~~ON HOLD~~	+	==Crystal structure of HMP-1-HMP-2 complex==
-		+	<StructureSection load='5xa5' size='340' side='right' caption='[[5xa5]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XA5 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [http://pdbe.org/5xa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [http://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref> [[http://www.uniprot.org/uniprot/HMP2_CAEEL HMP2_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation.<ref>PMID:9531567</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Choi, H J]]
		+	[[Category: Hardin, J]]
		+	[[Category: Kang, H]]
		+	[[Category: Shao, X]]
		+	[[Category: Weis, W I]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Five-helix bundle]]

OCA: Protected "5xa5" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 12:24:31 GMT

Protected "5xa5" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:24, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5xa5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Apr 2017 12:24:30 GMT

New page: '''Unreleased structure''' The entry 5xa5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5xa5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]