5xby - Revision history

OCA at 07:59, 22 November 2023

OCA — Wed, 22 Nov 2023 07:59:00 GMT

←Older revision		Revision as of 07:59, 22 November 2023
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	==Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)==		==Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)==
-	<StructureSection load='5xby' size='340' side='right' caption='[[5xby]], [[Resolution\|resolution]] 3.25Å' scene=''>	+	<StructureSection load='5xby' size='340' side='right'caption='[[5xby]], [[Resolution\|resolution]] 3.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xby]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XBY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xby]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XBY FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>spa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1280~~ "~~Micrococcus aureus~~" ~~(Rosenbach 1884) Zopf 1885~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.25Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xby OCA], [~~http~~://pdbe.org/5xby PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xby RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xby PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xby ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xby OCA], [https://pdbe.org/5xby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xby RCSB], [https://www.ebi.ac.uk/pdbsum/5xby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xby ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KAP2_HUMAN KAP2_HUMAN]] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.	+	[https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU] [https://www.uniprot.org/uniprot/KAP2_HUMAN KAP2_HUMAN] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Kim, J H~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Kwon, N Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lee, H~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Lee, J H~~]]	+	[[Category: Kim JH]]
-	[[Category: ~~Lee, J O~~]]	+	[[Category: Kwon NY]]
-	[[Category: ~~Youn, S J~~]]	+	[[Category: Lee H]]
-	[[Category: ~~Kinase~~]]	+	[[Category: Lee JH]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Lee JO]]
-	[[Category: ~~Protein binding]]~~	+	[[Category: Youn SJ]]
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Synthetic protein~~]]	+

OCA at 06:51, 16 November 2017

OCA — Thu, 16 Nov 2017 06:51:15 GMT

←Older revision		Revision as of 06:51, 16 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xby is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)==
		+	<StructureSection load='5xby' size='340' side='right' caption='[[5xby]], [[Resolution\|resolution]] 3.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xby]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XBY FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xby OCA], [http://pdbe.org/5xby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xby RCSB], [http://www.ebi.ac.uk/pdbsum/5xby PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xby ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAP2_HUMAN KAP2_HUMAN]] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Generating artificial protein assemblies with complex shapes requires a method for connecting protein components with stable and predictable structures. Currently available methods for creating rigid protein assemblies rely on either complicated calculations or extensive trial and error. We describe a simple and efficient method for connecting two proteins via a fused alpha helix that is formed by joining two preexisting helices into a single extended helix. Because the end-to-end ligation of helices does not guarantee the formation of a continuous helix, we superimposed 1-2 turns of pairs of connecting helices by using a molecular graphics program. Then, we chose amino acids from the two natural sequences that would stabilize the connecting helix. This "shared helix method" is highly efficient. All the designed proteins that could be produced in Escherichia coli were readily crystallized and had the expected fusion structures. To prove the usefulness of this method, we produced two novel repeat proteins by assembling several copies of natural or artificial proteins with alpha helices at both termini. Their crystal structures demonstrated the successful assembly of the repeating units with the intended curved shapes. We propose that this method could dramatically expand the available repertoire of natural repeat proteins.

-	~~Authors:~~ Youn~~, S.J.~~, Kwon~~, N.Y.~~, Lee~~, J.H.~~, Kim, J~~.H.~~, Lee, H., Lee~~, J~~.O.	+	Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.,Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO Sci Rep. 2017 Jun 1;7(1):2595. doi: 10.1038/s41598-017-02803-z. PMID:28572639<ref>PMID:28572639</ref>

-	~~Description: Crystal structure~~ of the ~~PKA~~-~~Protein A fusion protein (end~~-~~to-end fusion)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Youn, S.J]]~~	+	<div class="pdbe-citations 5xby" style="background-color:#fffaf0;"></div>
-	[[Category: Kim, J.H]]	+	== References ==
-	[[Category: ~~Lee~~, ~~J.O~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kim, J H]]
		+	[[Category: Kwon, N Y]]
	[[Category: Lee, H]]		[[Category: Lee, H]]
-	[[Category: Lee, J.H]]	+	[[Category: Lee, J H]]
-	[[Category: ~~Kwon~~, ~~N.Y~~]]	+	[[Category: Lee, J O]]
		+	[[Category: Youn, S J]]
		+	[[Category: Kinase]]
		+	[[Category: Lyase]]
		+	[[Category: Protein binding]]
		+	[[Category: Signaling protein]]
		+	[[Category: Synthetic protein]]

OCA at 12:02, 4 May 2017

OCA — Thu, 04 May 2017 12:02:33 GMT

←Older revision		Revision as of 12:02, 4 May 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xby is ON HOLD	+	The entry 5xby is ON HOLD until Paper Publication

	Authors: Youn, S.J., Kwon, N.Y., Lee, J.H., Kim, J.H., Lee, H., Lee, J.O.		Authors: Youn, S.J., Kwon, N.Y., Lee, J.H., Kim, J.H., Lee, H., Lee, J.O.

OCA: Protected "5xby" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 12:28:15 GMT

Protected "5xby" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:28, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5xby is ON HOLD Authors: Youn, S.J., Kwon, N.Y., Lee, J.H., Kim, J.H., Lee, H., Lee, J.O. Description: Crystal structure of the PKA-Protein A fusio...

OCA — Wed, 05 Apr 2017 12:28:14 GMT

New page: '''Unreleased structure''' The entry 5xby is ON HOLD Authors: Youn, S.J., Kwon, N.Y., Lee, J.H., Kim, J.H., Lee, H., Lee, J.O. Description: Crystal structure of the PKA-Protein A fusio...

New page

'''Unreleased structure'''

The entry 5xby is ON HOLD

Authors: Youn, S.J., Kwon, N.Y., Lee, J.H., Kim, J.H., Lee, H., Lee, J.O.

Description: Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)
[[Category: Unreleased Structures]]
[[Category: Youn, S.J]]
[[Category: Kim, J.H]]
[[Category: Lee, J.O]]
[[Category: Lee, H]]
[[Category: Lee, J.H]]
[[Category: Kwon, N.Y]]