5xfz - Revision history

OCA at 08:01, 22 November 2023

OCA — Wed, 22 Nov 2023 08:01:35 GMT

←Older revision		Revision as of 08:01, 22 November 2023
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	==Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6==		==Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6==
-	<StructureSection load='5xfz' size='340' side='right' caption='[[5xfz]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='5xfz' size='340' side='right'caption='[[5xfz]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Idesa Idesa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xg0\|5xg0]]~~, [[~~5xfx~~\|~~5xfx]], [[5xfy\|5xfy~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ISF6_4831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1547922 IDESA])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [https://pdbe.org/5xfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [https://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [~~http~~://pdbe.org/5xfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PETH_IDESA PETH_IDESA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Idesa~~]]	+	[[Category: Ideonella sakaiensis]]
-	[[Category: ~~Chen, C C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Guo, R T~~]]	+	[[Category: Chen CC]]
-	[[Category: ~~Han, X~~]]	+	[[Category: Guo RT]]
-	[[Category: ~~Liu, W D~~]]	+	[[Category: Han X]]
-	[[Category: ~~Zheng, Y Y~~]]	+	[[Category: Liu WD]]
-	[[Category: ~~Acidocalcisomal pyrophosphatase]]~~	+	[[Category: Zheng YY]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Substrate binding~~]]	+

OCA at 08:57, 27 December 2017

OCA — Wed, 27 Dec 2017 08:57:28 GMT

←Older revision		Revision as of 08:57, 27 December 2017
Line 3:		Line 3:
	<StructureSection load='5xfz' size='340' side='right' caption='[[5xfz]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='5xfz' size='340' side='right' caption='[[5xfz]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Idesa Idesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xg0\|5xg0]], [[5xfx\|5xfx]], [[5xfy\|5xfy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xg0\|5xg0]], [[5xfx\|5xfx]], [[5xfy\|5xfy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISF6_4831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1547922 IDESA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [http://pdbe.org/5xfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [http://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [http://pdbe.org/5xfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [http://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
		+
		+	Structural insight into catalytic mechanism of PET hydrolase.,Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT Nat Commun. 2017 Dec 13;8(1):2106. doi: 10.1038/s41467-017-02255-z. PMID:29235460<ref>PMID:29235460</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xfz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Idesa]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Guo, R T]]		[[Category: Guo, R T]]

OCA at 06:20, 20 December 2017

OCA — Wed, 20 Dec 2017 06:20:16 GMT

←Older revision		Revision as of 06:20, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xfz is ~~ON HOLD until Apr 11 2019~~	+	==Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6==
-		+	<StructureSection load='5xfz' size='340' side='right' caption='[[5xfz]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~Authors~~: ~~Han~~, X., ~~Liu~~, W.D., ~~Zheng~~, Y.Y., ~~Chen, C~~.C., ~~Guo, R~~.T.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of double mutant of a novel esterase~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xg0\|5xg0]], [[5xfx\|5xfx]], [[5xfy\|5xfy]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [http://pdbe.org/5xfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [http://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, C C]]
		+	[[Category: Guo, R T]]
	[[Category: Han, X]]		[[Category: Han, X]]
-	[[Category: ~~Chen~~, ~~C.C~~]]	+	[[Category: Liu, W D]]
-	[[Category: ~~Liu~~, ~~W.D~~]]	+	[[Category: Zheng, Y Y]]
-	[[Category: ~~Guo, R.T~~]]	+	[[Category: Acidocalcisomal pyrophosphatase]]
-	[[Category: ~~Zheng, Y.Y~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Inhibitor]]
		+	[[Category: Metal-binding]]
		+	[[Category: Substrate binding]]

OCA at 15:26, 27 September 2017

OCA — Wed, 27 Sep 2017 15:26:51 GMT

←Older revision		Revision as of 15:26, 27 September 2017
Line 7:		Line 7:
	Description: Crystal structure of double mutant of a novel esterase		Description: Crystal structure of double mutant of a novel esterase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Han, X]]
	[[Category: Chen, C.C]]		[[Category: Chen, C.C]]
	[[Category: Liu, W.D]]		[[Category: Liu, W.D]]
	[[Category: Guo, R.T]]		[[Category: Guo, R.T]]
-	[[Category: Han, X]]
	[[Category: Zheng, Y.Y]]		[[Category: Zheng, Y.Y]]

OCA at 13:02, 27 April 2017

OCA — Thu, 27 Apr 2017 13:02:02 GMT

←Older revision		Revision as of 13:02, 27 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xfz is ON HOLD	+	The entry 5xfz is ON HOLD until Apr 11 2019

	Authors: Han, X., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.		Authors: Han, X., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

OCA: Protected "5xfz" [edit=sysop:move=sysop]

OCA — Wed, 19 Apr 2017 12:09:18 GMT

Protected "5xfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:09, 19 April 2017

OCA: New page: '''Unreleased structure''' The entry 5xfz is ON HOLD Authors: Han, X., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of double mutant of a novel esterase...

OCA — Wed, 19 Apr 2017 12:09:17 GMT

New page: '''Unreleased structure''' The entry 5xfz is ON HOLD Authors: Han, X., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of double mutant of a novel esterase...

New page

'''Unreleased structure'''

The entry 5xfz is ON HOLD

Authors: Han, X., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

Description: Crystal structure of double mutant of a novel esterase
[[Category: Unreleased Structures]]
[[Category: Chen, C.C]]
[[Category: Liu, W.D]]
[[Category: Guo, R.T]]
[[Category: Han, X]]
[[Category: Zheng, Y.Y]]