5xop - Revision history

OCA at 08:08, 22 November 2023

OCA — Wed, 22 Nov 2023 08:08:19 GMT

←Older revision		Revision as of 08:08, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5xop' size='340' side='right'caption='[[5xop]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5xop' size='340' side='right'caption='[[5xop]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xop]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XOP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xop]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica_HM-1:IMSS-B Entamoeba histolytica HM-1:IMSS-B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XOP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xop OCA], [~~http~~://pdbe.org/5xop PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xop RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xop PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xop ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xop OCA], [https://pdbe.org/5xop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xop RCSB], [https://www.ebi.ac.uk/pdbsum/5xop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xop ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M3TKH6_ENTH1 M3TKH6_ENTH1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Entamoeba histolytica HM-1:IMSS-B]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gourinath, S]]	+	[[Category: Gourinath S]]
-	[[Category: Kumar, S]]	+	[[Category: Kumar S]]
-	~~[[Category: Cabp1]]~~	+
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Ef-hand]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 06:56, 14 October 2020

OCA — Wed, 14 Oct 2020 06:56:03 GMT

←Older revision		Revision as of 06:56, 14 October 2020
Line 1:		Line 1:

	==Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant==		==Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant==
-	<StructureSection load='5xop' size='340' side='right' caption='[[5xop]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5xop' size='340' side='right'caption='[[5xop]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xop]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOP OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XOP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xop]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XOP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xop OCA], [http://pdbe.org/5xop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xop RCSB], [http://www.ebi.ac.uk/pdbsum/5xop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xop ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xop OCA], [http://pdbe.org/5xop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xop RCSB], [http://www.ebi.ac.uk/pdbsum/5xop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xop ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 5xop" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xop" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tailspike protein\|Tailspike protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gourinath, S]]		[[Category: Gourinath, S]]
	[[Category: Kumar, S]]		[[Category: Kumar, S]]

OCA at 07:21, 6 December 2017

OCA — Wed, 06 Dec 2017 07:21:01 GMT

←Older revision		Revision as of 07:21, 6 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xop is ~~ON HOLD until Nov 30 2018~~	+	==Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant==
		+	<StructureSection load='5xop' size='340' side='right' caption='[[5xop]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xop]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xop OCA], [http://pdbe.org/5xop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xop RCSB], [http://www.ebi.ac.uk/pdbsum/5xop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xop ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium plays a pivotal role in the pathogenesis of amoebiasis, a major disease caused by Entamoeba histolytica. Several EF-hand containing calcium-binding proteins (CaBPs) have been identified from E. histolytica. Even though these proteins have very high sequence similarity, they bind to different target proteins in a Ca2+ dependent manner, leading to different functional pathways (Yadava et al., Mol Biochem Parasito 1997;84:69-82; Chakrabarty et al., J Biol Chem 2004;279:12898-12908) The crystal structure of the Entamoeba histolytica calcium binding protein-1 (EhCaBP1) has been determined at 2.4 A resolution. The crystals were grown using MPD as precipitant and they belong to P6(3) space group with unit cell parameters of a = 95.25 A, b = 95.25 A, c = 64.99 A. Only two out of the four expected EF hand motifs could be modeled into the electron density map and the final model refined to R factor of 25.6% and Free_R of 28%. Unlike CaM, the first two EF hand motifs in EhCaBP1 are connected by a long helix and form a dumbbell shaped structure. Owing to domain swapping oligomerization three EhCaBP1 molecules interact in a head to tail manner to form a triangular trimer. This arrangement allows the EF-hand motif of one molecule to interact with that of an adjacent molecule to form a two EF-hand domain similar to that seen in the N-terminal domain of the NMR structure of CaBP1, calmodulin and troponin C. The oligomeric state of EhCaBP1 results in reduced flexibility between domains and may be responsible for the more limited set of targets recognized by EhCaBP1.

-	~~Authors~~: ~~Kumar~~, S., ~~Gourinath~~, S.	+	Crystal structure of calcium binding protein-1 from Entamoeba histolytica: a novel arrangement of EF hand motifs.,Kumar S, Padhan N, Alam N, Gourinath S Proteins. 2007 Sep 1;68(4):990-8. PMID:17554780<ref>PMID:17554780</ref>

-	~~Description: Crystal Structure~~ of N-~~terminal domain EhCaBP1 EF~~-~~2 mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Kumar, S]]~~	+	<div class="pdbe-citations 5xop" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gourinath, S]]		[[Category: Gourinath, S]]
		+	[[Category: Kumar, S]]
		+	[[Category: Cabp1]]
		+	[[Category: Calcium binding]]
		+	[[Category: Ef-hand]]
		+	[[Category: Metal binding protein]]

OCA: Protected "5xop" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:38:18 GMT

Protected "5xop" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:38, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xop is ON HOLD until Nov 30 2018 Authors: Kumar, S., Gourinath, S. Description: Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant [[Categ...

OCA — Wed, 27 Sep 2017 15:38:17 GMT

New page: '''Unreleased structure''' The entry 5xop is ON HOLD until Nov 30 2018 Authors: Kumar, S., Gourinath, S. Description: Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant [[Categ...

New page

'''Unreleased structure'''

The entry 5xop is ON HOLD until Nov 30 2018

Authors: Kumar, S., Gourinath, S.

Description: Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant
[[Category: Unreleased Structures]]
[[Category: Kumar, S]]
[[Category: Gourinath, S]]