5xox - Revision history

OCA at 08:08, 22 November 2023

OCA — Wed, 22 Nov 2023 08:08:36 GMT

←Older revision		Revision as of 08:08, 22 November 2023
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	<StructureSection load='5xox' size='340' side='right'caption='[[5xox]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5xox' size='340' side='right'caption='[[5xox]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xox]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XOX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xox]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XOX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA(His)_guanylyltransferase tRNA(His) guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.79 2.7.7.79] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xox OCA], [~~http~~://pdbe.org/5xox PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xox RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xox PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xox ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xox OCA], [https://pdbe.org/5xox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xox RCSB], [https://www.ebi.ac.uk/pdbsum/5xox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xox ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/THG1_YEAST THG1_YEAST]] Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.<ref>PMID:14633974</ref>	+	[https://www.uniprot.org/uniprot/THG1_YEAST THG1_YEAST] Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.<ref>PMID:14633974</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hwang, K Y~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Lee, E H~~]]	+	[[Category: Hwang KY]]
-	[[Category: Lee~~, K~~]]	+	[[Category: Lee EH]]
-	[[Category: ~~Son, J~~]]	+	[[Category: Lee K]]
-	[[Category: ~~Gtp]]~~	+	[[Category: Son J]]
-	~~[[Category: Post-transcriptional modification]]~~	+
-	~~[[Category: Reverse polymerization]]~~	+
-	~~[[Category: Thg1]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:56, 14 October 2020

OCA — Wed, 14 Oct 2020 06:56:15 GMT

←Older revision		Revision as of 06:56, 14 October 2020
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	==Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae==		==Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae==
-	<StructureSection load='5xox' size='340' side='right' caption='[[5xox]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5xox' size='340' side='right'caption='[[5xox]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xox]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XOX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xox]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XOX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA(His)_guanylyltransferase tRNA(His) guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.79 2.7.7.79] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA(His)_guanylyltransferase tRNA(His) guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.79 2.7.7.79] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xox OCA], [http://pdbe.org/5xox PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xox RCSB], [http://www.ebi.ac.uk/pdbsum/5xox PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xox ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xox OCA], [http://pdbe.org/5xox PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xox RCSB], [http://www.ebi.ac.uk/pdbsum/5xox PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xox ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hwang, K Y]]		[[Category: Hwang, K Y]]
	[[Category: Lee, E H]]		[[Category: Lee, E H]]

OCA: Protected "5xox" [edit=sysop:move=sysop]

OCA — Thu, 17 Aug 2017 06:04:23 GMT

Protected "5xox" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:04, 17 August 2017

OCA: New page: ==Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae== == Structural highlights ==
<table><tr><td colspan='2'>[[5xox]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA(His)_guanylyltransferase tRNA(His) guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.79 2.7.7.79] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xox OCA], [http://pdbe.org/5xox PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xox RCSB], [http://www.ebi.ac.uk/pdbsum/5xox PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xox ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/THG1_YEAST THG1_YEAST]] Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.<ref>PMID:14633974</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
tRNA maturation involves several steps, including processing, splicing, CCA addition, and posttranscriptional modifications. tRNAHis guanylyltransferase (Thg1) is the only enzyme known to catalyze templated nucleotide addition in the 3'-5' direction, unlike other DNA and RNA polymerases. For a better understanding of its unique catalytic mechanism at the molecular level, we determined the crystal structure of GTP-bound Thg1 from Saccharomyces cerevisiae at the maximum resolution of 3.0 A. The structure revealed the enzyme to have a tetrameric conformation that is well conserved among different species, and the GTP molecule was clearly bound at the active site, coordinating with two magnesium ions. In addition, two flexible protomers at the potential binding site (PBS) for tRNAHis were observed. We suggest that the PBS of the tetramer could also be one of the sites for interaction with partner proteins.

Crystal structure of tRNAHis guanylyltransferase from Saccharomyces cerevisiae.,Lee K, Lee EH, Son J, Hwang KY Biochem Biophys Res Commun. 2017 Aug 19;490(2):400-405. doi:, 10.1016/j.bbrc.2017.06.054. Epub 2017 Jun 13. PMID:28623126<ref>PMID:28623126</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xox" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Hwang, K Y]]
[[Category: Lee, E H]]
[[Category: Lee, K]]
[[Category: Son, J]]
[[Category: Gtp]]
[[Category: Post-transcriptional modification]]
[[Category: Reverse polymerization]]
[[Category: Thg1]]
[[Category: Transferase]]