5xrn - Revision history

OCA at 10:20, 27 March 2024

OCA — Wed, 27 Mar 2024 10:20:04 GMT

←Older revision		Revision as of 10:20, 27 March 2024
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	==Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure==		==Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure==
-	<StructureSection load='5xrn' size='340' side='right' caption='[[5xrn]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5xrn' size='340' side='right'caption='[[5xrn]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xrn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XRN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xrn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XRN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CLC, LGALS10, LGALS10A ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrn OCA], [~~http~~://pdbe.org/5xrn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xrn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xrn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrn OCA], [https://pdbe.org/5xrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xrn RCSB], [https://www.ebi.ac.uk/pdbsum/5xrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN]] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>	+	[https://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Galectin-10 (Gal-10) which forms Charcot-Leyden crystals in vivo, is crucial to regulating lymph cell function. Here, we solved the crystal structures of Gal-10 and eight variants at resolutions of 1.55-2.00 A. Structural analysis and size exclusion chromatography demonstrated that Gal-10 dimerizes with a novel global shape that is different from that of other prototype galectins (e.g., Gal-1, -2 and -7). In the Gal-10 dimer, Glu33 from one subunit modifies the carbohydrate binding site of another, essentially inhibiting disaccharide binding. Nevertheless, glycerol (and possibly other small hydroxylated molecules) can interact with residues at the ligand binding site, with His53 being the most crucial for binding. Alanine substitution of the conserved Trp residue (Trp72) that is crucial to saccharide binding in other galectins, actually leads to enhanced erythrocyte agglutination, suggesting that Trp72 negatively regulates Gal-10 ligand binding. Overall, our crystallographic and biochemical results provide insight into Gal-10 ligand binding specificity.	+

-	Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding.,Su J, Gao J, Si Y, Cui L, Song C, Wang Y, Wu R, Tai G, Zhou Y Glycobiology. 2017 Dec 23. pii: 4773962. doi: 10.1093/glycob/cwx107. PMID:29293962<ref>PMID:29293962</ref>	+	==See Also==
-		+	*[[Galectin 3D structures\|Galectin 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 5xrn" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Su, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Galectin-10/charcot-leyden crystal protein]]~~	+	[[Category: Su J]]
-	~~[[Category: Protein binding~~]]	+

OCA at 06:18, 8 March 2018

OCA — Thu, 08 Mar 2018 06:18:18 GMT

←Older revision		Revision as of 06:18, 8 March 2018
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	<StructureSection load='5xrn' size='340' side='right' caption='[[5xrn]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5xrn' size='340' side='right' caption='[[5xrn]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xrn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xrn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLC, LGALS10, LGALS10A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrn OCA], [http://pdbe.org/5xrn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrn RCSB], [http://www.ebi.ac.uk/pdbsum/5xrn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrn OCA], [http://pdbe.org/5xrn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrn RCSB], [http://www.ebi.ac.uk/pdbsum/5xrn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrn ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Su, J]]		[[Category: Su, J]]
	[[Category: Galectin-10/charcot-leyden crystal protein]]		[[Category: Galectin-10/charcot-leyden crystal protein]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 18:26, 24 January 2018

OCA — Wed, 24 Jan 2018 18:26:50 GMT

←Older revision		Revision as of 18:26, 24 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xrn is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure==
		+	<StructureSection load='5xrn' size='340' side='right' caption='[[5xrn]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xrn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrn OCA], [http://pdbe.org/5xrn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrn RCSB], [http://www.ebi.ac.uk/pdbsum/5xrn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN]] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Galectin-10 (Gal-10) which forms Charcot-Leyden crystals in vivo, is crucial to regulating lymph cell function. Here, we solved the crystal structures of Gal-10 and eight variants at resolutions of 1.55-2.00 A. Structural analysis and size exclusion chromatography demonstrated that Gal-10 dimerizes with a novel global shape that is different from that of other prototype galectins (e.g., Gal-1, -2 and -7). In the Gal-10 dimer, Glu33 from one subunit modifies the carbohydrate binding site of another, essentially inhibiting disaccharide binding. Nevertheless, glycerol (and possibly other small hydroxylated molecules) can interact with residues at the ligand binding site, with His53 being the most crucial for binding. Alanine substitution of the conserved Trp residue (Trp72) that is crucial to saccharide binding in other galectins, actually leads to enhanced erythrocyte agglutination, suggesting that Trp72 negatively regulates Gal-10 ligand binding. Overall, our crystallographic and biochemical results provide insight into Gal-10 ligand binding specificity.

-	~~Authors~~: Su, J.	+	Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding.,Su J, Gao J, Si Y, Cui L, Song C, Wang Y, Wu R, Tai G, Zhou Y Glycobiology. 2017 Dec 23. pii: 4773962. doi: 10.1093/glycob/cwx107. PMID:29293962<ref>PMID:29293962</ref>

-	~~Description: Galectin-10~~/~~Charcot-Leyden crystal protein variant K73A crystal structure~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5xrn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Su, J]]		[[Category: Su, J]]
		+	[[Category: Galectin-10/charcot-leyden crystal protein]]
		+	[[Category: Protein binding]]

OCA: Protected "5xrn" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:42:58 GMT

Protected "5xrn" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:42, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xrn is ON HOLD until Paper Publication Authors: Su, J. Description: Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure [[Ca...

OCA — Wed, 27 Sep 2017 15:42:57 GMT

New page: '''Unreleased structure''' The entry 5xrn is ON HOLD until Paper Publication Authors: Su, J. Description: Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure [[Ca...

New page

'''Unreleased structure'''

The entry 5xrn is ON HOLD until Paper Publication

Authors: Su, J.

Description: Galectin-10/Charcot-Leyden crystal protein variant K73A crystal structure
[[Category: Unreleased Structures]]
[[Category: Su, J]]