5xwa - Revision history

OCA at 08:16, 22 November 2023

OCA — Wed, 22 Nov 2023 08:16:35 GMT

←Older revision		Revision as of 08:16, 22 November 2023
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	<StructureSection load='5xwa' size='340' side='right'caption='[[5xwa]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5xwa' size='340' side='right'caption='[[5xwa]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xwa]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xwa]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Capsicum_annuum Capsicum annuum] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XWA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwa OCA], [https://pdbe.org/5xwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xwa RCSB], [https://www.ebi.ac.uk/pdbsum/5xwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwa ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwa OCA], [~~http~~://pdbe.org/5xwa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xwa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xwa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwa ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Capsicum annuum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	~~[[Category: Trypsin]]~~	+	[[Category: Bhoite AS]]
-	[[Category: Bhoite~~, A S~~]]	+	[[Category: Giri AP]]
-	[[Category: Giri~~, A P~~]]	+	[[Category: Kulkarni KA]]
-	[[Category: Kulkarni~~, K A~~]]	+	[[Category: Saikhedkar NS]]
-	[[Category: Saikhedkar~~, N S]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Protease~~]]	+

OCA at 07:00, 14 October 2020

OCA — Wed, 14 Oct 2020 07:00:06 GMT

←Older revision		Revision as of 07:00, 14 October 2020
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	==Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10==		==Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10==
-	<StructureSection load='5xwa' size='340' side='right' caption='[[5xwa]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5xwa' size='340' side='right'caption='[[5xwa]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xwa]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xwa]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XWA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwa OCA], [http://pdbe.org/5xwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwa RCSB], [http://www.ebi.ac.uk/pdbsum/5xwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwa OCA], [http://pdbe.org/5xwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwa RCSB], [http://www.ebi.ac.uk/pdbsum/5xwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5xwa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xwa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 06:34, 28 March 2018

OCA — Wed, 28 Mar 2018 06:34:23 GMT

←Older revision		Revision as of 06:34, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xwa is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10==
		+	<StructureSection load='5xwa' size='340' side='right' caption='[[5xwa]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xwa]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XWA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwa OCA], [http://pdbe.org/5xwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwa RCSB], [http://www.ebi.ac.uk/pdbsum/5xwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwa ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Potato type II protease inhibitors (Pin-II PIs) impede the growth of lepidopteran insects by inhibiting serine protease-like enzymes in the larval gut. The three amino acid reactive centre loop (RCL) of these proteinaceous inhibitors is crucial for protease binding and is conserved across the Pin-II family. However, the molecular mechanism and inhibitory potential of the RCL tripeptides in isolation of the native protein has remained elusive. In this study, six peptides corresponding to the RCLs of the predominant Pin-II PIs were identified, synthesized and evaluated for in vitro and in vivo inhibitory activity against serine proteases of the polyphagous insect, Helicoverpa armigera. RCL peptides with sequences PRN, PRY and TRE were found to be potent inhibitors that adversely affected the growth and development of H. armigera. The binding mechanism and differential affinity of the RCL peptides with serine proteases was delineated by crystal structures of complexes of the RCL peptides with trypsin. Residues P1 and P2 of the inhibitors play a crucial role in the interaction and specificity of these inhibitors. Important features of RCL peptides like higher inhibition of insect proteases, enhanced efficacy at alkaline gut pH, longer retention and high stability in insect gut make them suitable molecules for the development of sustainable pest management strategies for crop protection.

-	~~Authors:~~ Saikhedkar, ~~N.S.~~, Bhoite, ~~A.S.~~, ~~Giri~~, ~~A.P.~~, Kulkarni, K.A.	+	Tripeptides derived from reactive centre loop of potato type II protease inhibitors preferentially inhibit midgut proteases of Helicoverpa armigera.,Saikhedkar NS, Joshi RS, Bhoite AS, Mohandasan R, Yadav AK, Fernandes M, Kulkarni KA, Giri AP Insect Biochem Mol Biol. 2018 Feb 25;95:17-25. doi: 10.1016/j.ibmb.2018.02.001. PMID:29486250<ref>PMID:29486250</ref>

-	~~Description: Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor~~, ~~PRY, at pH 10~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Bhoite, A.S]]	+	<div class="pdbe-citations 5xwa" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Saikhedkar~~, ~~N.S~~]]	+	== References ==
-	[[Category: Kulkarni, K.A]]	+	<references/>
-	[[Category: ~~Giri~~, ~~A.P~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Sus scrofa]]
		+	[[Category: Trypsin]]
		+	[[Category: Bhoite, A S]]
		+	[[Category: Giri, A P]]
		+	[[Category: Kulkarni, K A]]
		+	[[Category: Saikhedkar, N S]]
		+	[[Category: Complex]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Inhibitor]]
		+	[[Category: Protease]]

OCA at 05:06, 15 February 2018

OCA — Thu, 15 Feb 2018 05:06:27 GMT

←Older revision		Revision as of 05:06, 15 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xwa is ON HOLD	+	The entry 5xwa is ON HOLD until Paper Publication

	Authors: Saikhedkar, N.S., Bhoite, A.S., Giri, A.P., Kulkarni, K.A.		Authors: Saikhedkar, N.S., Bhoite, A.S., Giri, A.P., Kulkarni, K.A.
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	Description: Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10		Description: Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Bhoite, A.S]]
	[[Category: Saikhedkar, N.S]]		[[Category: Saikhedkar, N.S]]
	[[Category: Kulkarni, K.A]]		[[Category: Kulkarni, K.A]]
	[[Category: Giri, A.P]]		[[Category: Giri, A.P]]
-	[[Category: Bhoite, A.S]]

OCA: Protected "5xwa" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:51:12 GMT

Protected "5xwa" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:51, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xwa is ON HOLD Authors: Saikhedkar, N.S., Bhoite, A.S., Giri, A.P., Kulkarni, K.A. Description: Crystal Structure of Porcine pancreatic trypsin wi...

OCA — Wed, 27 Sep 2017 15:51:11 GMT

New page: '''Unreleased structure''' The entry 5xwa is ON HOLD Authors: Saikhedkar, N.S., Bhoite, A.S., Giri, A.P., Kulkarni, K.A. Description: Crystal Structure of Porcine pancreatic trypsin wi...

New page

'''Unreleased structure'''

The entry 5xwa is ON HOLD

Authors: Saikhedkar, N.S., Bhoite, A.S., Giri, A.P., Kulkarni, K.A.

Description: Crystal Structure of Porcine pancreatic trypsin with tripeptide inhibitor, PRY, at pH 10
[[Category: Unreleased Structures]]
[[Category: Saikhedkar, N.S]]
[[Category: Kulkarni, K.A]]
[[Category: Giri, A.P]]
[[Category: Bhoite, A.S]]