5xwv - Revision history

OCA at 08:16, 22 November 2023

OCA — Wed, 22 Nov 2023 08:16:48 GMT

←Older revision		Revision as of 08:16, 22 November 2023
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	==Substrate-bound Structure of a Ketoreductase from the Second Module of the amphotericin Polyketide Synthases==		==Substrate-bound Structure of a Ketoreductase from the Second Module of the amphotericin Polyketide Synthases==
-	<StructureSection load='5xwv' size='340' side='right' caption='[[5xwv]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5xwv' size='340' side='right'caption='[[5xwv]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xwv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1459 As 4.1459~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XWV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xwv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_nodosus Streptomyces nodosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XWV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8H6:S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl]+(2R)-2-methyl-3-oxidanylidene-pentanethioate'>8H6</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amphB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40318 AS 4.1459])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8H6:S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl]+(2R)-2-methyl-3-oxidanylidene-pentanethioate'>8H6</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwv OCA], [~~http~~://pdbe.org/5xwv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xwv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xwv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwv OCA], [https://pdbe.org/5xwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xwv RCSB], [https://www.ebi.ac.uk/pdbsum/5xwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93NW7_9ACTN Q93NW7_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~As 4 1459~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Liu, C~~]]	+	[[Category: Streptomyces nodosus]]
-	[[Category: ~~Zheng, J~~]]	+	[[Category: Liu C]]
-	[[Category: ~~Ketoreductase]]~~	+	[[Category: Zheng J]]
-	~~[[Category: Modular polyketide synthease]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:35, 4 July 2018

OCA — Wed, 04 Jul 2018 07:35:10 GMT

←Older revision		Revision as of 07:35, 4 July 2018
Line 3:		Line 3:
	<StructureSection load='5xwv' size='340' side='right' caption='[[5xwv]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5xwv' size='340' side='right' caption='[[5xwv]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xwv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XWV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xwv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1459 As 4.1459]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XWV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8H6:S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl]+(2R)-2-methyl-3-oxidanylidene-pentanethioate'>8H6</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8H6:S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl]+(2R)-2-methyl-3-oxidanylidene-pentanethioate'>8H6</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amphB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40318 AS 4.1459])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwv OCA], [http://pdbe.org/5xwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwv RCSB], [http://www.ebi.ac.uk/pdbsum/5xwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwv OCA], [http://pdbe.org/5xwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwv RCSB], [http://www.ebi.ac.uk/pdbsum/5xwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwv ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1459]]
	[[Category: Liu, C]]		[[Category: Liu, C]]
	[[Category: Zheng, J]]		[[Category: Zheng, J]]

OCA at 05:52, 6 June 2018

OCA — Wed, 06 Jun 2018 05:52:13 GMT

←Older revision		Revision as of 05:52, 6 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xwv is ~~ON HOLD until Paper~~ Publication	+	==Substrate-bound Structure of a Ketoreductase from the Second Module of the amphotericin Polyketide Synthases==
		+	<StructureSection load='5xwv' size='340' side='right' caption='[[5xwv]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xwv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XWV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8H6:S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl]+(2R)-2-methyl-3-oxidanylidene-pentanethioate'>8H6</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xwv OCA], [http://pdbe.org/5xwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xwv RCSB], [http://www.ebi.ac.uk/pdbsum/5xwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xwv ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ketoreductase (KR) domains of modular polyketide synthases (PKSs) control the stereochemistry of C2 methyl and C3 hydroxyl substituents of polyketide intermediates. To understand the molecular basis of stereocontrol exerted by KRs, the crystal structure of a KR from the second module of the amphotericin PKS (AmpKR2) complexed with NADP(+) and 2-methyl-3-oxopentanoyl-pantetheine was solved. This first ternary structure provides direct evidence to the hypothesis that a substrate enters into the active site of an A-type KR from the side opposite the coenzyme to generate an L-hydroxyl substituent. A comparison with the ternary complex of a G355T/Q364H mutant sheds light on the structural basis for stereospecificity toward the substrate C2 methyl substituent. Functional assays suggest the pantetheine handle shows obvious influence on the catalytic efficiency and the stereochemical outcome. Together, these findings extend our current understanding of the stereochemical control of PKS KR domains.

-	~~Authors: Liu~~, C., ~~Zheng~~, J.	+	Substrate-bound structures of a ketoreductase from amphotericin modular polyketide synthase.,Liu C, Yuan M, Xu X, Wang L, Keatinge-Clay AT, Deng Z, Lin S, Zheng J J Struct Biol. 2018 Apr 4. pii: S1047-8477(18)30091-1. doi:, 10.1016/j.jsb.2018.04.001. PMID:29626512<ref>PMID:29626512</ref>

-	~~Description: Substrate-bound Structure of~~ a ~~Ketoreductase from the Second Module~~ of the ~~amphotericin Polyketide Synthases~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5xwv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Liu, C]]		[[Category: Liu, C]]
	[[Category: Zheng, J]]		[[Category: Zheng, J]]
		+	[[Category: Ketoreductase]]
		+	[[Category: Modular polyketide synthease]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5xwv" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:52:14 GMT

Protected "5xwv" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:52, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xwv is ON HOLD until Paper Publication Authors: Liu, C., Zheng, J. Description: Substrate-bound Structure of a Ketoreductase from the Second Modul...

OCA — Wed, 27 Sep 2017 15:52:13 GMT

New page: '''Unreleased structure''' The entry 5xwv is ON HOLD until Paper Publication Authors: Liu, C., Zheng, J. Description: Substrate-bound Structure of a Ketoreductase from the Second Modul...

New page

'''Unreleased structure'''

The entry 5xwv is ON HOLD until Paper Publication

Authors: Liu, C., Zheng, J.

Description: Substrate-bound Structure of a Ketoreductase from the Second Module of the amphotericin Polyketide Synthases
[[Category: Unreleased Structures]]
[[Category: Liu, C]]
[[Category: Zheng, J]]