5xyg - Revision history

OCA at 08:17, 22 November 2023

OCA — Wed, 22 Nov 2023 08:17:26 GMT

←Older revision		Revision as of 08:17, 22 November 2023
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	==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.==		==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.==
-	<StructureSection load='5xyg' size='340' side='right'caption='[[5xyg]]' scene=''>	+	<StructureSection load='5xyg' size='340' side='right'caption='[[5xyg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_sp. Arthrobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyg OCA], [~~http~~://pdbe.org/5xyg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xyg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xyg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyg OCA], [https://pdbe.org/5xyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyg RCSB], [https://www.ebi.ac.uk/pdbsum/5xyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NYLC_FLASK NYLC_FLASK]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nylon hydrolase (NylC) is initially expressed as an inactive precursor (36 kDa). The precursor is cleaved autocatalytically at Asn266/Thr267 to generate an active enzyme composed of an alpha subunit (27 kDa) and a beta subunit (9 kDa). Four alphabeta heterodimers (molecules A-D) form a doughnut-shaped quaternary structure. In this study, the thermostability of the parental NylC was altered by amino acid substitutions located at the A/D interface (D122G/H130Y/D36A/L137A) or the A/B interface (E263Q) and spanned a range of 47 degrees C. Considering structural, biophysical, and biochemical analyses, we discuss the structural basis of the stability of nylon hydrolase. From the analytical centrifugation data obtained regarding the various mutant enzymes, we conclude that the assembly of the monomeric units is dynamically altered by the mutations. Finally, we propose a model that can predict whether the fate of the nascent polypeptide will be correct subunit assembly, inappropriate protein-protein interactions causing aggregation, or intracellular degradation of the polypeptide.
		+
		+	Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase.,Negoro S, Shibata N, Lee YH, Takehara I, Kinugasa R, Nagai K, Tanaka Y, Kato DI, Takeo M, Goto Y, Higuchi Y Sci Rep. 2018 Jun 27;8(1):9725. doi: 10.1038/s41598-018-27860-w. PMID:29950566<ref>PMID:29950566</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xyg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arthrobacter sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Higuchi Y]]		[[Category: Higuchi Y]]

OCA at 07:01, 14 October 2020

OCA — Wed, 14 Oct 2020 07:01:21 GMT

←Older revision		Revision as of 07:01, 14 October 2020
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-	'''Unreleased structure'''

-	~~The entry 5xyg is ON HOLD until Paper Publication~~	+	==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.==
-		+	<StructureSection load='5xyg' size='340' side='right'caption='[[5xyg]]' scene=''>
-	~~Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XYG FirstGlance]. <br>
-	~~Description:~~ Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyg OCA], [http://pdbe.org/5xyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyg RCSB], [http://www.ebi.ac.uk/pdbsum/5xyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyg ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Shibata~~, N]]	+	__TOC__
-	[[Category: ~~Negoro, S~~]]	+	</StructureSection>
-	[[Category: Higuchi, Y]]	+	[[Category: Large Structures]]
-	[[Category: Nagai, K]]	+	[[Category: Higuchi Y]]
		+	[[Category: Nagai K]]
		+	[[Category: Negoro S]]
		+	[[Category: Shibata N]]

OCA at 05:03, 31 October 2018

OCA — Wed, 31 Oct 2018 05:03:50 GMT

←Older revision		Revision as of 05:03, 31 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xyg is ON HOLD	+	The entry 5xyg is ON HOLD until Paper Publication

	Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.		Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.
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	Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.		Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Shibata, N]]
	[[Category: Negoro, S]]		[[Category: Negoro, S]]
	[[Category: Higuchi, Y]]		[[Category: Higuchi, Y]]
	[[Category: Nagai, K]]		[[Category: Nagai, K]]
-	[[Category: Shibata, N]]

OCA: Protected "5xyg" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:55:03 GMT

Protected "5xyg" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xyg is ON HOLD Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y. Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrob...

OCA — Wed, 27 Sep 2017 15:55:01 GMT

New page: '''Unreleased structure''' The entry 5xyg is ON HOLD Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y. Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrob...

New page

'''Unreleased structure'''

The entry 5xyg is ON HOLD

Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.

Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.
[[Category: Unreleased Structures]]
[[Category: Negoro, S]]
[[Category: Higuchi, Y]]
[[Category: Nagai, K]]
[[Category: Shibata, N]]