5xyv - Revision history

OCA at 08:17, 22 November 2023

OCA — Wed, 22 Nov 2023 08:17:41 GMT

←Older revision		Revision as of 08:17, 22 November 2023
Line 1:		Line 1:

	==Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain==		==Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain==
-	<StructureSection load='5xyv' size='340' side='right' caption='[[5xyv]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5xyv' size='340' side='right'caption='[[5xyv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYV FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>rhi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~7227 DROME])~~, ~~del, CG9252 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=7227 DROME])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [~~http~~://pdbe.org/5xyv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [https://pdbe.org/5xyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [https://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~DEL_DROME DEL_DROME~~]] Developmental protein involved in oogenesis (PubMed:16616913). Required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females (PubMed:16616913). Also required for dorso-ventral and antero-posterior patterning of oocyte and eggshell (PubMed:16616913). May be involved in microtubule function during oogenesis (PubMed:16616913). Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers (PubMed:28847004). Component of the RDC complex (rhi, del and cuff) which binds to repressive H3K9me3 marks in the piRNA clusters (PubMed:28847004). RDC promotes the bidirectional transcription of piRNA clusters at these sites by interacting with Moonshiner which forms a complex with the transcription initiation factors TfIIA-S and Trf2 (PubMed:28847004). This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark (PubMed:28847004).<ref>PMID:16616913</ref> <ref>PMID:28847004</ref>	+	[https://www.uniprot.org/uniprot/Q7JXA8_DROME Q7JXA8_DROME]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
-	[[Category: ~~Huang, Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Yu, B W~~]]	+	[[Category: Huang Y]]
-	[[Category: ~~Chromoshadow]]~~	+	[[Category: Yu BW]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Pirna pathway]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 09:35, 18 July 2018

OCA — Wed, 18 Jul 2018 09:35:36 GMT

←Older revision		Revision as of 09:35, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='5xyv' size='340' side='right' caption='[[5xyv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5xyv' size='340' side='right' caption='[[5xyv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [http://pdbe.org/5xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [http://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rhi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), del, CG9252 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [http://pdbe.org/5xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [http://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
	[[Category: Huang, Y]]		[[Category: Huang, Y]]
	[[Category: Yu, B W]]		[[Category: Yu, B W]]

OCA at 05:43, 20 June 2018

OCA — Wed, 20 Jun 2018 05:43:07 GMT

←Older revision		Revision as of 05:43, 20 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xyv is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain==
		+	<StructureSection load='5xyv' size='340' side='right' caption='[[5xyv]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYV FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [http://pdbe.org/5xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [http://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DEL_DROME DEL_DROME]] Developmental protein involved in oogenesis (PubMed:16616913). Required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females (PubMed:16616913). Also required for dorso-ventral and antero-posterior patterning of oocyte and eggshell (PubMed:16616913). May be involved in microtubule function during oogenesis (PubMed:16616913). Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers (PubMed:28847004). Component of the RDC complex (rhi, del and cuff) which binds to repressive H3K9me3 marks in the piRNA clusters (PubMed:28847004). RDC promotes the bidirectional transcription of piRNA clusters at these sites by interacting with Moonshiner which forms a complex with the transcription initiation factors TfIIA-S and Trf2 (PubMed:28847004). This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark (PubMed:28847004).<ref>PMID:16616913</ref> <ref>PMID:28847004</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.

-	~~Authors: Yu~~, B~~.W.~~, ~~Huang~~, Y.	+	Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487<ref>PMID:29858487</ref>

-	~~Description: Crystal structure~~ of ~~drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Yu, B.W]]~~	+	<div class="pdbe-citations 5xyv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Huang, Y]]		[[Category: Huang, Y]]
		+	[[Category: Yu, B W]]
		+	[[Category: Chromoshadow]]
		+	[[Category: Complex]]
		+	[[Category: Pirna pathway]]
		+	[[Category: Protein binding]]

OCA: Protected "5xyv" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:55:44 GMT

Protected "5xyv" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xyv is ON HOLD until Paper Publication Authors: Yu, B.W., Huang, Y. Description: Crystal structure of drosophila melanogaster Rhino chromoshadow d...

OCA — Wed, 27 Sep 2017 15:55:43 GMT

New page: '''Unreleased structure''' The entry 5xyv is ON HOLD until Paper Publication Authors: Yu, B.W., Huang, Y. Description: Crystal structure of drosophila melanogaster Rhino chromoshadow d...

New page

'''Unreleased structure'''

The entry 5xyv is ON HOLD until Paper Publication

Authors: Yu, B.W., Huang, Y.

Description: Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain
[[Category: Unreleased Structures]]
[[Category: Yu, B.W]]
[[Category: Huang, Y]]