5xza - Revision history

OCA at 08:18, 22 November 2023

OCA — Wed, 22 Nov 2023 08:18:03 GMT

←Older revision		Revision as of 08:18, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5xza' size='340' side='right'caption='[[5xza]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5xza' size='340' side='right'caption='[[5xza]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xza]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp.~~_aureus_st228~~ Staphylococcus aureus subsp. aureus ~~st228~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XZA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~pfkA, SAOUHSC_01807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=1074919 Staphylococcus aureus subsp. aureus ST228])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xza OCA], [https://pdbe.org/5xza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xza RCSB], [https://www.ebi.ac.uk/pdbsum/5xza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xza ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xza OCA], [~~http~~://pdbe.org/5xza PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xza RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xza PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xza ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PFKA_STAA8 PFKA_STAA8]~~] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339]	+	[https://www.uniprot.org/uniprot/PFKA_STAAN PFKA_STAAN] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5xza" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xza" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphofructokinase 3D structures\|Phosphofructokinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 6-phosphofructokinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Staphylococcus aureus subsp. aureus ~~st228~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-	[[Category: Tian, T]]	+	[[Category: Tian T]]
-	[[Category: Wang~~, C L~~]]	+	[[Category: Wang CL]]
-	[[Category: Zang~~, J Y]]~~	+	[[Category: Zang JY]]
-	~~[[Category: Phosphofructokinase]]~~	+
-	~~[[Category: Staphylococcus aureus]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:23, 23 May 2019

OCA — Thu, 23 May 2019 07:23:56 GMT

←Older revision		Revision as of 07:23, 23 May 2019
Line 3:		Line 3:
	<StructureSection load='5xza' size='340' side='right'caption='[[5xza]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5xza' size='340' side='right'caption='[[5xza]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xza]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xza]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_st228 Staphylococcus aureus subsp. aureus st228]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XZA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pfkA, SAOUHSC_01807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1074919 Staphylococcus aureus subsp. aureus ST228])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xza OCA], [http://pdbe.org/5xza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xza RCSB], [http://www.ebi.ac.uk/pdbsum/5xza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xza ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xza OCA], [http://pdbe.org/5xza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xza RCSB], [http://www.ebi.ac.uk/pdbsum/5xza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xza ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~A0A0D1I869_STAAU A0A0D1I869_STAAU~~]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339~~][SAAS:SAAS01078966~~]	+	[[http://www.uniprot.org/uniprot/PFKA_STAA8 PFKA_STAA8]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus subsp. aureus st228]]
	[[Category: Tian, T]]		[[Category: Tian, T]]
	[[Category: Wang, C L]]		[[Category: Wang, C L]]

OCA at 11:51, 13 March 2019

OCA — Wed, 13 Mar 2019 11:51:18 GMT

←Older revision		Revision as of 11:51, 13 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xza is ~~ON HOLD~~	+	==Crystal Structure of Phosphofructokinase from Staphylococcus aureus in complex with ADP==
		+	<StructureSection load='5xza' size='340' side='right'caption='[[5xza]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xza]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XZA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xza OCA], [http://pdbe.org/5xza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xza RCSB], [http://www.ebi.ac.uk/pdbsum/5xza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xza ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A0D1I869_STAAU A0A0D1I869_STAAU]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339][SAAS:SAAS01078966]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most reported bacterial phosphofructokinases (Pfks) are tetramers that exhibit activity allosterically regulated via conformational changes between the R and T states. We report that the Pfk from Staphylococcus aureus NCTC 8325 ( SaPfk) exists as both an active tetramer and an inactive dimer in solution. Multiple effectors, including pH, ADP, ATP, and adenylyl-imidodiphosphate (AMP-PNP), cause equilibrium shifts from the tetramer to dimer, whereas the substrate F6P stabilizes SaPfk tetrameric assembly. Crystal structures of SaPfk in complex with different ligands and biochemical analysis reveal that the flexibility of the Gly150-Leu151 motif in helix alpha7 plays a role in tetramer-dimer conversion. Thus, we propose a molecular mechanism for allosteric regulation of bacterial Pfk via conversion between the tetramer and dimer in addition to the well-characterized R-state/T-state mechanism.

-	~~Authors: Wang, C.L~~., Tian, T., ~~Zang~~, J.Y.	+	Structural Insights into the Regulation of Staphylococcus aureus Phosphofructokinase by Tetramer-Dimer Conversion.,Tian T, Wang C, Wu M, Zhang X, Zang J Biochemistry. 2018 Jul 24;57(29):4252-4262. doi: 10.1021/acs.biochem.8b00028., Epub 2018 Jul 9. PMID:29940104<ref>PMID:29940104</ref>

-	~~Description: Crystal Structure~~ of ~~Phosphofructokinase from Staphylococcus aureus in complex with ADP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Wang, C.L~~]]	+	<div class="pdbe-citations 5xza" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zang, J.Y~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 6-phosphofructokinase]]
		+	[[Category: Large Structures]]
	[[Category: Tian, T]]		[[Category: Tian, T]]
		+	[[Category: Wang, C L]]
		+	[[Category: Zang, J Y]]
		+	[[Category: Phosphofructokinase]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Transferase]]

OCA: Protected "5xza" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:56:55 GMT

Protected "5xza" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:56, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xza is ON HOLD Authors: Wang, C.L., Tian, T., Zang, J.Y. Description: Crystal Structure of Phosphofructokinase from Staphylococcus aureus in compl...

OCA — Wed, 27 Sep 2017 15:56:54 GMT

New page: '''Unreleased structure''' The entry 5xza is ON HOLD Authors: Wang, C.L., Tian, T., Zang, J.Y. Description: Crystal Structure of Phosphofructokinase from Staphylococcus aureus in compl...

New page

'''Unreleased structure'''

The entry 5xza is ON HOLD

Authors: Wang, C.L., Tian, T., Zang, J.Y.

Description: Crystal Structure of Phosphofructokinase from Staphylococcus aureus in complex with ADP
[[Category: Unreleased Structures]]
[[Category: Wang, C.L]]
[[Category: Zang, J.Y]]
[[Category: Tian, T]]