5xzw - Revision history

OCA at 07:01, 14 October 2020

OCA — Wed, 14 Oct 2020 07:01:58 GMT

←Older revision		Revision as of 07:01, 14 October 2020
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	==Crystal structure of Rad53 1-466==		==Crystal structure of Rad53 1-466==
-	<StructureSection load='5xzw' size='340' side='right' caption='[[5xzw]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5xzw' size='340' side='right'caption='[[5xzw]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xzw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XZW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xzw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XZW FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dual-specificity_kinase Dual-specificity kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.12.1 2.7.12.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dual-specificity_kinase Dual-specificity kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.12.1 2.7.12.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xzw OCA], [http://pdbe.org/5xzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xzw RCSB], [http://www.ebi.ac.uk/pdbsum/5xzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xzw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xzw OCA], [http://pdbe.org/5xzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xzw RCSB], [http://www.ebi.ac.uk/pdbsum/5xzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xzw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5xzw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xzw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Protein kinase Spk1\|Protein kinase Spk1]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Dual-specificity kinase]]		[[Category: Dual-specificity kinase]]
		+	[[Category: Large Structures]]
	[[Category: Tsai, M D]]		[[Category: Tsai, M D]]
	[[Category: Weng, J H]]		[[Category: Weng, J H]]

OCA at 06:54, 11 October 2017

OCA — Wed, 11 Oct 2017 06:54:16 GMT

←Older revision		Revision as of 06:54, 11 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xzw is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Rad53 1-466==
		+	<StructureSection load='5xzw' size='340' side='right' caption='[[5xzw]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xzw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XZW FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dual-specificity_kinase Dual-specificity kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.12.1 2.7.12.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xzw OCA], [http://pdbe.org/5xzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xzw RCSB], [http://www.ebi.ac.uk/pdbsum/5xzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xzw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAD53_YEAST RAD53_YEAST]] Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.<ref>PMID:8355715</ref> <ref>PMID:7958905</ref> <ref>PMID:10550056</ref> <ref>PMID:11809875</ref> <ref>PMID:15024067</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The vast majority of in vitro structural and functional studies of the activation mechanism of protein kinases use the kinase domain alone. Well-demonstrated effects of regulatory domains or allosteric factors are scarce for serine/threonine kinases. Here we use a site-specifically phosphorylated SCD1-FHA1-kinase three-domain construct of the serine/threonine kinase Rad53 to show the effect of phospho-priming, an in vivo regulatory mechanism, on the autophosphorylation intermediate and specificity. Unphosphorylated Rad53 is a flexible monomer in solution but is captured in an asymmetric enzyme:substrate complex in crystal with the two FHA domains separated from each other. Phospho-priming induces formation of a stable dimer via intermolecular pT-FHA binding in solution. Importantly, autophosphorylation of unprimed and phospho-primed Rad53 produced predominantly inactive pS350-Rad53 and active pT354-Rad53, respectively. The latter mechanism was also demonstrated in vivo. Our results show that, while Rad53 can display active conformations under various conditions, simulation of in vivo regulatory conditions confers functionally relevant autophosphorylation.

-	~~Authors~~:	+	Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation.,Chen ES, Weng JH, Chen YH, Wang SC, Liu XX, Huang WC, Matsui T, Kawano Y, Liao JH, Lim LH, Bessho Y, Huang KF, Wu WJ, Tsai MD Biochemistry. 2017 Sep 26;56(38):5112-5124. doi: 10.1021/acs.biochem.7b00689., Epub 2017 Sep 15. PMID:28858528<ref>PMID:28858528</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5xzw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dual-specificity kinase]]
		+	[[Category: Tsai, M D]]
		+	[[Category: Weng, J H]]
		+	[[Category: Checkpoint kinase]]
		+	[[Category: Fha domain]]
		+	[[Category: Serine/threonine-protein kinase]]
		+	[[Category: Transferase]]

OCA: Protected "5xzw" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:58:58 GMT

Protected "5xzw" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:58, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xzw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 15:58:57 GMT

New page: '''Unreleased structure''' The entry 5xzw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5xzw is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]